{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T03:15:07Z","timestamp":1777518907413,"version":"3.51.4"},"reference-count":15,"publisher":"FapUNIFESP (SciELO)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Soc. Bras. Med. Trop."],"published-print":{"date-parts":[[2002,6]]},"abstract":"<jats:p>Devido a crescente import\u00e2ncia dos cocc\u00eddios intestinais (Cryptosporidium, Isospora e Cyclospora) como parasitos oportunistas, \u00e9 fundamental para os laborat\u00f3rios diferenciar morfologicamente estes protozo\u00e1rios; a t\u00e9cnica de Ziehl-Neelsen modificada (ZNm) \u00e9 amplamente utilizada para este fim; recentemente, foi proposto um novo procedimento, a colora\u00e7\u00e3o combinada do \u00e1cido tricr\u00f4mico (Acid-Fast-Trichrome - AFT). O objetivo do presente estudo foi comparar os processos AFT e ZNm para a detec\u00e7\u00e3o destes cocc\u00eddios em amostras fecais de pacientes portadores do v\u00edrus VIH. Foram selecionados dois grupos de indiv\u00edduos, para inclus\u00e3o no estudo, segundo a presen\u00e7a (n=60) ou aus\u00eancia de diarr\u00e9ia (n=60). As amostras de fezes foram coletadas em solu\u00e7\u00e3o de formalina 10% e os esfrega\u00e7os fecais preparados i) diretamente das fezes e ii) ap\u00f3s concentra\u00e7\u00e3o pr\u00e9via a 500xg (10 minutos), foram submetidos aos diferentes processos de colora\u00e7\u00e3o. Considerando-se a positividade por t\u00e9cnica (AFT e ZNm), verificou-se a superioridade do procedimento de ZNm (n=19; 100% dos casos positivos) sobre o de AFT (n=8; 42,1%). Ambos possibilitaram a identifica\u00e7\u00e3o dos 101 casos verdadeiramente negativos. Coccidiose intestinal foi mais frequente entre os pacientes que apresentaram diarr\u00e9ia (26,6%) em compara\u00e7\u00e3o \u00e0 positividade observada entre os ind\u00edviduos assintom\u00e1ticos (5%) sendo que C. cayetanensis n\u00e3o foi detectada em ambos os grupos. Foi de nosso interesse avaliar a aplicabilidade da t\u00e9cnica AFT para a colora\u00e7\u00e3o deste protozo\u00e1rio. Devido \u00e0 sensibilidade e especificidade obtida neste estudo (100%), conclui-se que o m\u00e9todo de ZNm continua sendo o mais indicado para o diagn\u00f3stico da criptosporidiose e isosporose, principalmente quando associado ao procedimento de centr\u00edfugo-concentra\u00e7\u00e3o (500xg, 10 minutos). Embora a colora\u00e7\u00e3o AFT tenha baixo custo, faz-se necess\u00e1rio o seu aperfei\u00e7oamento pois este procedimento permite o diagn\u00f3stico simult\u00e2neo dos cocc\u00eddios intestinais (C. parvum, I. belli e C. cayetanensis) e dos microspor\u00eddios.<\/jats:p>","DOI":"10.1590\/s0037-86822002000300002","type":"journal-article","created":{"date-parts":[[2005,4,26]],"date-time":"2005-04-26T16:49:11Z","timestamp":1114534151000},"page":"209-214","source":"Crossref","is-referenced-by-count":14,"title":["Compara\u00e7\u00e3o entre os m\u00e9todos de Ziehl-Neelsen modificado e Acid-Fast-Trichrome para a pesquisa fecal de Cryptosporidium parvum e Isospora belli"],"prefix":"10.1590","volume":"35","author":[{"given":"Carla R.","family":"Rigo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regina Maura B.","family":"Franco","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Campinas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","series-title":"Infections of the Gastrointestinal Tract","first-page":"1107","volume-title":"Cryptosporidium and Related Species","author":"Adal KA","year":"1995"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1016\/0020-7519(96)00057-4","article-title":"Comparison of 2 Centrifugation Procedures in the Formalin - Ethyl Acetate Stool Concentracion Technique for the Detection of Cryptosporidium oocysts","volume":"26","author":"Clavel A","year":"1996","journal-title":"International Journal of Parasitology"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1136\/jcp.44.3.182","article-title":"Opportunistic Protozoan Infections in Human Immunodeficiency Virus Disease: Review Highlighting Diagnostic and Therapeutic Aspects","volume":"44","author":"Curry A","year":"1991","journal-title":"Journal of Clinical Pathology","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9746","issn-type":"print"},{"key":"ref4","series-title":"Parasitologia Cl\u00ednica: Diagn\u00f3stico de Laborat\u00f3rio dos Cocc\u00eddios e Microspor\u00eddios Intestinais","isbn-type":"print","author":"De Carli GA","year":"2000","ISBN":"https:\/\/id.crossref.org\/isbn\/857430102107"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"429","DOI":"10.7326\/0003-4819-124-4-199602150-00008","article-title":"Understanding Intestinal Spore-Forming Protozoa: Cryptosporidia, Microsporidia, Isospora, and Cyclospora","volume":"124","author":"Goodgame RW","year":"1996","journal-title":"Annals of Internal Medicine","ISSN":"https:\/\/id.crossref.org\/issn\/0003-4819","issn-type":"print"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/S0065-308X(08)60117-7","article-title":"Human Cryptosporidiosis: Epidemiology, Transmission, Clinical Disease, Treatment and Diagnosis","volume":"40","author":"Griffiths JK","year":"1998","journal-title":"Advances in Parasitology","ISSN":"https:\/\/id.crossref.org\/issn\/0065-308X","issn-type":"print"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1186\/BF03548684","article-title":"Staining of Cryptosporidia by a Modified Ziehl-Neelsen Technique","volume":"22","author":"Henriksen SA","year":"1981","journal-title":"Acta Veterinaria Scandinavia"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1007\/BF01709253","article-title":"Efficacy of Different Methods for Detection of Low Cryptosporidium parvum Oocysts Numbers or Antigen Concentrations in Stool Specimens","volume":"16","author":"Ignatius R","year":"1997","journal-title":"European Journal of Microbiology and Infectious Diseases"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1128\/JCM.35.2.446-449.1997","article-title":"A New Acid-Fast-Trichrome Stain for Simultaneous Detection of Cryptosporidium parvum and Microsporidial Species in Stool Specimens","volume":"35","author":"Ignatius R","year":"1997","journal-title":"Journal of Clinical Microbiology","ISSN":"https:\/\/id.crossref.org\/issn\/0095-1137","issn-type":"print"},{"key":"ref10","first-page":"S57","article-title":"Cryptosporidiosis: Source of infection and Guidelines for Preventions","volume":"I)","author":"Juranek DD","year":"1996","journal-title":"Clinical Infectious Diseases 21 (supl"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1128\/JCM.37.3.835-837.1999","article-title":"Justification for Use of a Single Trichrome Stain as the Sole Means for Routine Detection of Intestinal Parasites in Concentrated Stool Specimens","volume":"37","author":"Kellogg JA","year":"1999","journal-title":"Journal of Clinical Microbiology","ISSN":"https:\/\/id.crossref.org\/issn\/0095-1137","issn-type":"print"},{"key":"ref12","series-title":"Infections of the Gastrointestinal Tract.","first-page":"483","author":"Smith PD","year":"1995"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1093\/clinids\/23.3.429","article-title":"Cyclospora: an Overview","volume":"23","author":"Soave R","year":"1996","journal-title":"Clinical Infectious Diseases","ISSN":"https:\/\/id.crossref.org\/issn\/1058-4838","issn-type":"print"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1881","DOI":"10.1016\/S0020-7519(98)00146-5","article-title":"Fuchsin fluorescence and autofluorescence in Cryptosporidium, Isospora and Cyclospora oocysts","volume":"28","author":"Varea M","year":"1998","journal-title":"International Journal of Parasitology"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1128\/JCM.35.3.730-733.1997","article-title":"Uniform Staining of Cyclospora Oocysts in Fecal Smears by a Modified Safranin Technique with Microwave Heating","volume":"35","author":"Visvesvara GS","year":"1997","journal-title":"Journal of Clinical Microbiology","ISSN":"https:\/\/id.crossref.org\/issn\/0095-1137","issn-type":"print"}],"container-title":["Revista da Sociedade Brasileira de Medicina Tropical"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rsbmt\/v35n3\/a02v35n3.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,9]],"date-time":"2021-07-09T21:25:17Z","timestamp":1625865917000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0037-86822002000300002&lng=pt&tlng=pt"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,6]]},"references-count":15,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2002,6]]}},"alternative-id":["S0037-86822002000300002"],"URL":"https:\/\/doi.org\/10.1590\/s0037-86822002000300002","relation":{},"ISSN":["0037-8682"],"issn-type":[{"value":"0037-8682","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,6]]}}}