{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T21:28:26Z","timestamp":1791494906720,"version":"4.3.4"},"reference-count":30,"publisher":"FapUNIFESP (SciELO)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Soc. Bras. Med. Trop."],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:p>INTRODU\u00c7\u00c3O: Desconhecida a realidade da leishmaniose visceral canina em Garanhuns, objetivou-se investigar a ocorr\u00eancia de anticorpos antileishmania spp em c\u00e3es domiciliados e semidomiciliados e os poss\u00edveis fatores de risco envolvidos. M\u00c9TODOS: Em uma primeira etapa foram coletadas 256 amostras de sangue de c\u00e3es que foram submetidas \u00e0 rea\u00e7\u00e3o de imunofluoresc\u00eancia indireta (RIFI) na dilui\u00e7\u00e3o 1:40. Adicionalmente, 23 amostras positivas na RIFI foram testadas com um teste r\u00e1pido imunocromatogr\u00e1fico. Em uma segunda etapa, novas amostras de sangue de 18 c\u00e3es positivos na RIFI na primeira fase do estudo foram coletadas, retestadas pela RIFI (1:40 e 1:80) e, adicionalmente, pela rea\u00e7\u00e3o em cadeia da polimerase para pesquisa de DNA de Leishmania infantum. Ademais, 16 dessas amostras foram retestadas pelo teste r\u00e1pido imunocromatogr\u00e1fico. RESULTADOS: Na primeira etapa, 16% das amostras foram positivas na RIFI (1:40) e apenas tr\u00eas (13%) foram positivas no teste r\u00e1pido imunocromatogr\u00e1fico. Na segunda etapa, 12 amostras foram positivas na RIFI na dilui\u00e7\u00e3o 1:40 e sete tamb\u00e9m na dilui\u00e7\u00e3o 1:80. Nenhuma amostra foi positiva na rea\u00e7\u00e3o em cadeia da polimerase e no teste r\u00e1pido imunocromatogr\u00e1fico. Sinais cl\u00ednicos de leishmaniose visceral ocorreram em 4,9% dos c\u00e3es positivos. N\u00e3o houve diferen\u00e7a estat\u00edstica entre idade, sexo e status cl\u00ednico dos c\u00e3es, por\u00e9m entre seus locais de origem. CONCLUS\u00d5ES: Os c\u00e3es domiciliados e semidomiciliados de Garanhuns apresentam anticorpos antileishmania spp, sendo, em sua grande maioria, assintom\u00e1ticos.<\/jats:p>","DOI":"10.1590\/s0037-86822010000100010","type":"journal-article","created":{"date-parts":[[2010,3,17]],"date-time":"2010-03-17T12:51:52Z","timestamp":1268830312000},"page":"41-45","source":"Crossref","is-referenced-by-count":24,"title":["Preval\u00eancia de anticorpos antileishmania spp em c\u00e3es de Garanhuns, Agreste de Pernambuco"],"prefix":"10.1590","volume":"43","author":[{"given":"Jessica Maria Leite dos","family":"Santos","sequence":"first","affiliation":[{"name":"Universidade Federal Rural de Pernambuco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filipe","family":"Dantas-Torres","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz; Universit\u00e0 degli Studi di Bari, Italia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Renato Franzosi","family":"Mattos","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural de Pernambuco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe Ragner Lima","family":"Lino","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural de Pernambuco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edlian Sabrina Silvestre","family":"Andrade","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural de Pernambuco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rute Chami\u00e9 Alves de","family":"Souza","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural de Pernambuco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio Luiz da Cunha","family":"Brito","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural de Pernambuco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Edileuza Felinto de","family":"Brito","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sinval Pinto","family":"Brand\u00e3o-Filho","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucilene","family":"Sim\u00f5es-Mattos","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural de Pernambuco"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1590\/S0074-02762005000800001","article-title":"Lutzomyia longipalpis and the eco-epidemiology of American visceral leishmaniasis, with particular reference to Brazil: a review","volume":"100","author":"ainson R","year":"2005","journal-title":"Mem Inst Oswaldo 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