{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T02:56:52Z","timestamp":1717210612155},"reference-count":24,"publisher":"FapUNIFESP (SciELO)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Soc. Bras. Med. Trop."],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:p>INTRODU\u00c7\u00c3O: A candid\u00edase \u00e9 uma das infec\u00e7\u00f5es f\u00fangicas mais frequentes entre os pacientes infectados pelo v\u00edrus da imunodefici\u00eancia humana. O presente estudo objetivou a caracteriza\u00e7\u00e3o das leveduras do g\u00eanero Candida de distintas amostras cl\u00ednicas, provenientes de pacientes HIV - positivos, assim como a determina\u00e7\u00e3o do perfil de suscetibilidade in vitro a cinco drogas antif\u00fangicas. M\u00c9TODOS: A caracteriza\u00e7\u00e3o dos isolados de Candida sp foi realizada atrav\u00e9s da metodologia cl\u00e1ssica, testes bioqu\u00edmicos (zimograma e auxanograma) e morfol\u00f3gicos (prova do tubo germinativo e microcultivo em l\u00e2mina). Tamb\u00e9m, foram realizadas a t\u00e9cnica genot\u00edpica (PCR) e identifica\u00e7\u00e3o pelo m\u00e9todo comercial API 20C AUX (BioMeri\u00e9ux). Para a determina\u00e7\u00e3o do perfil de suscetibilidade in vitro, foram utilizadas cinco drogas antif\u00fangicas (cetoconazol, fluconazol, itraconazol, voriconazol e anfotericina B), atrav\u00e9s do m\u00e9todo comercialmente dispon\u00edvel - Etest. RESULTADOS: Foram identificados 105 isolados de leveduras do g\u00eanero Candida provenientes de 102 pacientes infectados pelo v\u00edrus HIV. Destes, foram caracterizadas 82 (78,1%) Candida albicans, 8 (7,6%) Candida parapsilosis, 8 (7,6%) Candida tropicalis, 4 (3,8%) Candida krusei, 2 (1,9%) Candida glabrata e 1 (1%) Candida guilliermondii. CONCLUS\u00d5ES: Considerando o perfil geral de sensibilidade, 60% dos isolados foram suscet\u00edveis a todos os antif\u00fangicos testados, por\u00e9m as esp\u00e9cies C. tropicalis e C. krusei demonstraram uma tend\u00eancia a valores mais elevados de CIMs para os az\u00f3is do que os encontrados paraC. albicans, sugerindo resist\u00eancia.<\/jats:p>","DOI":"10.1590\/s0037-86822010000600014","type":"journal-article","created":{"date-parts":[[2010,12,20]],"date-time":"2010-12-20T06:28:59Z","timestamp":1292826539000},"page":"673-677","source":"Crossref","is-referenced-by-count":9,"title":["Aspectos micol\u00f3gicos e suscetibilidade in vitro de leveduras do g\u00eanero Candida em pacientes HIV-positivos provenientes do Estado de Mato Grosso"],"prefix":"10.1590","volume":"43","author":[{"given":"Olivia Cometti","family":"Favalessa","sequence":"first","affiliation":[{"name":"Universidade Federal de Mato Grosso"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilena dos Anjos","family":"Martins","sequence":"additional","affiliation":[{"name":"Instituto Adolf Lutz"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosane Christine","family":"Hahn","sequence":"additional","affiliation":[{"name":"Universidade Federal de Mato Grosso; Universidade Federal de Mato Grosso, MT; Hospital Geral Universit\u00e1rio, MT"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","series-title":"Boletim Epidemiol\u00f3gico AIDS-DST","year":"2007"},{"key":"ref2","first-page":"515","article-title":"Susceptibilidad antif\u00fangica de Candida albicans recuperadas de pacientes com SIDA y candidiasis orofar\u00edngea y esof\u00e1gica","volume":"131","author":"Tapia C","year":"2003","journal-title":"Experience com Etest Rev Med Chile"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1034\/j.1600-0714.2002.310102.x","article-title":"Oral candidiasis as a clinical marker related to viral load, CD4 lymphocyte count and CD4 lynphocyte percentage in HIV-infected patients","volume":"31","author":"Campos J","year":"2002","journal-title":"J Oral Pathol Med","ISSN":"http:\/\/id.crossref.org\/issn\/0904-2512","issn-type":"print"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1034\/j.1600-0714.2002.310308.x","article-title":"Oral manifestations of an HIV positive cohort in the era of highly active anti-retroviral therapy (HAART) in South London","volume":"1","author":"Eyeson JD","year":"2002","journal-title":"J Oral Pathol Med","ISSN":"http:\/\/id.crossref.org\/issn\/0904-2512","issn-type":"print"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1590\/S0037-86822007000300004","article-title":"Identifica\u00e7\u00e3o microbiol\u00f3gica e sensibilidade in vitro de Candida isoladas da cavidade oral de indiv\u00edduos HIV positivos","volume":"40","author":"Wingeter MA","year":"2007","journal-title":"Rev Soc Bras Med Trop","ISSN":"http:\/\/id.crossref.org\/issn\/0037-8682","issn-type":"print"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"929","DOI":"10.2174\/138945005774912708","article-title":"Antifungal susceptibilidade testing methods","volume":"6","author":"Pfaller MA","year":"2005","journal-title":"Curr Drugs Targets"},{"key":"ref7","first-page":"128","article-title":"Evaluaci\u00f3n Del desempe\u00f1o diagn\u00f3stico Del m\u00e9dio de difusi\u00f3n em Agar Etest para El est\u00fadio de sensibilidad a los antif\u00fangicos","volume":"22","author":"Ballest\u00e9 R","year":"2006","journal-title":"Rev Med Urug","ISSN":"http:\/\/id.crossref.org\/issn\/0303-3295","issn-type":"print"},{"key":"ref8","series-title":"CLSI.M27-A3. 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