{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T22:34:22Z","timestamp":1766788462152},"reference-count":15,"publisher":"FapUNIFESP (SciELO)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Soc. Bras. Med. Trop."],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:p>INTRODUCTION: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. The common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. METHODS: In order to increase diagnosis efficacy, the PCR technique was improved. RESULTS AND CONCLUSIONS: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood filtration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears.<\/jats:p>","DOI":"10.1590\/s0037-86822011000300023","type":"journal-article","created":{"date-parts":[[2017,2,4]],"date-time":"2017-02-04T00:50:47Z","timestamp":1486169447000},"page":"380-382","source":"Crossref","is-referenced-by-count":11,"title":["Improvement of a PCR test to diagnose infection by Mansonella ozzardi"],"prefix":"10.1590","volume":"44","author":[{"given":"Luana Jana\u00edna Souza","family":"Vera","sequence":"first","affiliation":[{"name":"Faculdade S\u00e3o Lucas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio de Almeida","family":"Basano","sequence":"additional","affiliation":[{"name":"Faculdade S\u00e3o Lucas; Centro de Medicina Tropical de Rond\u00f4nia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana de Souza Almeida Aranha","family":"Camargo","sequence":"additional","affiliation":[{"name":"Faculdade S\u00e3o Lucas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andonai Krauze de","family":"Fran\u00e7a","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa em Patologias Tropicais"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo de Godoi Mattos","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz Noroeste"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Almeida Andrade","family":"Casseb","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa em Patologias Tropicais"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jansen Fernandes","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Pesquisas da Amaz\u00f4nia; 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