{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T11:52:17Z","timestamp":1779882737986,"version":"3.53.1"},"reference-count":35,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Soc. Bras. Med. Trop."],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:p>INTRODUCTION: Extended spectrum \u03b2-lactamases (ESBLs) are enzymes that degrade \u03b2-lactam antibiotics and have been reported to be an important cause of nosocomial infection in worldwide. METHODS: During 2009, 659 enterobacteria strains were isolated from different clinical specimens and tested for ESBL production. The disk approximation test, combined disk method and addition of clavulanic acid were used for phenotypic detection of the ESBL-producing strains and PCR for detection of the blaTEM and blaCTX-M genes. RESULTS: Among the isolates, 125 were ESBL producers. The blaCTX-M and blaTEM genes were detected in 90.4% and 75% of the strains, respectively. Most strains were isolated from urine. Klebsiella pneumoniae was the most prevalent organism. Microorganisms presented high resistance to the antibiotics. CONCLUSIONS: These results support the need for extending ESBL detection methods to different pathogens of the Enterobacteriaceae family because these methods are only currently standardized by the CLSI for Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca and Proteus mirabilis. Carbapenems were the antibiotic class of choice for the treatment of infections caused by ESBL-producing Enterobacteriaceae.<\/jats:p>","DOI":"10.1590\/s0037-86822011000400008","type":"journal-article","created":{"date-parts":[[2011,8,18]],"date-time":"2011-08-18T15:59:21Z","timestamp":1313683161000},"page":"441-446","source":"Crossref","is-referenced-by-count":19,"title":["Nosocomial infection and characterization of extended-spectrum \u03b2-lactamases-producing Enterobacteriaceae in Northeast Brazil"],"prefix":"10.1590","volume":"44","author":[{"given":"Afonso Gomes","family":"Abreu","sequence":"first","affiliation":[{"name":"Universidade Federal do Maranh\u00e3o"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirlei Garcia","family":"Marques","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rio","family":"Monteiro-Neto","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio do Maranh\u00e3o; Universidade Federal do Maranh\u00e3o"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto Morais Luz de","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Universidade Federal do Maranh\u00e3o"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azizedite Guedes","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Universidade Federal do Maranh\u00e3o; Universidade Federal do Maranh\u00e3o"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"key":"ref1","first-page":"295","article-title":"Extended-spectrum beta-lactamases (ESBLs) in Escherichia coli and Klebsiella pneumoniae: trends in the hospital and community settings","volume":"3","author":"Khanfar HS","year":"2009","journal-title":"J Infect Dev Ctries"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s10096-004-1264-8","article-title":"Prevalence and risk factors of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in an Israeli long-term care facility","volume":"24","author":"Mendelson G","year":"2005","journal-title":"Eur J Clin Microbiol Infect Dis","ISSN":"https:\/\/id.crossref.org\/issn\/0722-2211","issn-type":"print"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1007\/s00284-006-0307-z","article-title":"Prevalence of Community-Occurring Extended Spectrum \u03b2-Lactamase-Producing Enterobacteriaceae in Brazil","volume":"54","author":"Minarini LAR","year":"2007","journal-title":"Curr Microbiol","ISSN":"https:\/\/id.crossref.org\/issn\/0343-8651","issn-type":"print"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1128\/CMR.14.4.933-951.2001","article-title":"Extended-spectrum-\u03b2-lactamases in the 21st century: caracteriza\u00e7\u00e3o, epidemiology, and detection of this important resistance threat","volume":"14","author":"Bradford PA","year":"2001","journal-title":"Clin Microbiol Rev","ISSN":"https:\/\/id.crossref.org\/issn\/0893-8512","issn-type":"print"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1128\/CMR.18.4.657-686.2005","article-title":"Extended-spectrum \u03b2-lactamases: a clinical update","volume":"18","author":"Paterson DL","year":"2005","journal-title":"Clin Microbiol Rev","ISSN":"https:\/\/id.crossref.org\/issn\/0893-8512","issn-type":"print"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1590\/S1413-86702001000400006","article-title":"Pathogen frequency and resistance patterns in brazilian hospitals: summary of results from three years of the SENTRY Antimicrobial Surveillance Program","volume":"5","author":"Sader HS","year":"2001","journal-title":"Braz J Infect Dis"},{"key":"ref7","first-page":"344","article-title":"Extended-Spectrum \u03b2-lactamases in Klebsiella spp. and Escherichia coli obtained in a Brazilian teaching Hospital: detection, prevalence and molecular typing","volume":"34","author":"Freitas ALP","year":"2003","journal-title":"Braz J Microbiol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1046\/j.1469-0691.2003.00555.x","article-title":"Antimicrobial activity against strains of Escherichia coli and Klebsiella spp. with resistance phenotypes consistent with extended-spectrum \u03b2-lactamases in Europe","volume":"9","author":"Jones RN","year":"2003","journal-title":"Clin Microbiol Inf"},{"key":"ref9","series-title":"Performance standards for antimicrobial susceptibility testing: eighteenth informational supplement. 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