{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T15:24:25Z","timestamp":1769527465775,"version":"3.49.0"},"reference-count":33,"publisher":"FapUNIFESP (SciELO)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Soc. Bras. Med. Trop."],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:p>INTRODUCTION: Hospitals around the world have presented multiresistant Acinetobacter sp. outbreaks. The spread of these isolates that harbor an increasing variety of resistance genes makes the treatment of these infections and their control within the hospital environment more difficult. This study aimed to evaluate the occurrence and dissemination of Acinetobacter sp. multiresistant isolates and to identify acquired resistance genes. METHODS: We analyzed 274 clinical isolates of Acinetobacter sp. from five hospitals in Porto Alegre, RS, Brazil. We evaluated the susceptibility to antimicrobial, acquired resistance genes from Ambler's classes B and D, and performed molecular typing of the isolates using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) technique. RESULTS: A high (68%) percentage of multiresistant isolates of Acinetobacter sp. was observed, and 69% were resistant to carbapenems. We identified 84% of isolates belonging to species A. baumannii because they presented the gene blaOXA-51. The gene blaOXA-23 was detected in 62% of the isolates, and among these, 98% were resistant to carbapenems. Using the ERIC-PCR technique, we identified clones of Acinetobacter sp. spread among the four hospitals analyzed during the sampling period. CONCLUSIONS: The data indicate the dissemination of Acinetobacter sp. isolates among hospitals and their permanence in the hospital after one year.<\/jats:p>","DOI":"10.1590\/s0037-86822011000600014","type":"journal-article","created":{"date-parts":[[2012,1,7]],"date-time":"2012-01-07T07:04:31Z","timestamp":1325919871000},"page":"725-730","source":"Crossref","is-referenced-by-count":14,"title":["Molecular characterization of clinical multiresistant isolates of Acinetobacter sp. from hospitals in Porto Alegre, State of Rio Grande do Sul, Brazil"],"prefix":"10.1590","volume":"44","author":[{"given":"Alessandra Einsfeld","family":"Ferreira","sequence":"first","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Desir\u00e9e Padilha","family":"Marchetti","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela Rosa da","family":"Cunha","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lyvia Moreira de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daiane Bopp","family":"Fuentefria","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aline Gehlen Dall","family":"Bello","sequence":"additional","affiliation":[{"name":"Pontif\u00edcia Universidade do Rio Grande do Sul"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Afonso Luis","family":"Barth","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gertrudes","family":"Cor\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul; Universidade Federal do Rio Grande do Sul"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1128\/JB.96.1.39-42.1968","article-title":"Isolation of Acinetobacter from soil and water","volume":"96","author":"Baumann P","year":"1968","journal-title":"J Bacteriol","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9193","issn-type":"print"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"2214","DOI":"10.1128\/AEM.55.9.2214-2219.1989","article-title":"Detection of Acinetobacter sp. in rural drinking water supplies","volume":"55","author":"Bifulco J","year":"1989","journal-title":"Appl Environ Microbiol","ISSN":"https:\/\/id.crossref.org\/issn\/0099-2240","issn-type":"print"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1128\/AAC.47.5.1681-1688.2003","article-title":"Surveillance for antimicrobial susceptibility among clinical isolates of Pseudomonas aeruginosa an Acinetobacter baumannii from hospitalized patients in the United States, 1998-2001","volume":"47","author":"Karlowsky J","year":"2003","journal-title":"Antimicrob Agents Chemother","ISSN":"https:\/\/id.crossref.org\/issn\/0066-4804","issn-type":"print"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1938","DOI":"10.1128\/JCM.36.7.1938-1941.1998","article-title":"Survival of Acinetobacter baumannii on Dry Surfaces: Comparison of Outbreak and Sporadic Isolates","volume":"36","author":"Jawad A","year":"1998","journal-title":"J Clin Microbiol","ISSN":"https:\/\/id.crossref.org\/issn\/0095-1137","issn-type":"print"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1111\/j.1472-765X.2005.01791.x","article-title":"The emergence of multidrug resistant Acinetobacter species: a major concern in the hospital setting","volume":"41","author":"Hanlon G","year":"2005","journal-title":"Lett Appl Microbiol","ISSN":"https:\/\/id.crossref.org\/issn\/0266-8254","issn-type":"print"},{"key":"ref6","first-page":"16","article-title":"Dissemination of IMP-1 and OXA type b-lactamase in carbapenem-resistant Acinetobacter baumannii Korean","volume":"28","author":"Sung JY","year":"2008","journal-title":"J Lab Med"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1093\/jac\/dkn434","article-title":"Emergence and widespread dissemination of OXA-23: 24\/40 and -58 carbapenemases among Acinetobacter sp. in Asia-Pacific nations: report from the SENTRY Surveillance Program","volume":"63","author":"Mendes RE","year":"2009","journal-title":"J Antimicrob Chemoth"},{"key":"ref8","series-title":"M100-S17. Performance standards for antimicrobial susceptibility testing: 16th informational supplement","year":"2007"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1128\/JCM.38.1.40-43.2000","article-title":"Convenient Test for Screening Metallo-b-Lactamase producing Gram-Negative Bacteria by Using Thiol Compounds","volume":"38","author":"Arakawa Y","year":"2000","journal-title":"J Clin Microbiol","ISSN":"https:\/\/id.crossref.org\/issn\/0095-1137","issn-type":"print"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"3798","DOI":"10.1128\/JCM.40.10.3798-3801.2002","article-title":"Imipenem-EDTA Disk Method for Differentiation of Metallo-b-Lactamase-Producing Clinical Isolates of Pseudomonas sp. and Acinetobacter sp","volume":"40","author":"Yong D","year":"2002","journal-title":"J Clin Microbiol","ISSN":"https:\/\/id.crossref.org\/issn\/0095-1137","issn-type":"print"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"4623","DOI":"10.1128\/JCM.41.10.4623-4629.2003","article-title":"Evaluation of the Hodge Test and the Imipenem-EDTA Double-Disk Synergy Test for Differentiating Metallo-b-Lactamase-Producing Isolates of Pseudomonas sp. and Acinetobacter sp","volume":"41","author":"Lee K","year":"2003","journal-title":"J Clin Microbiol","ISSN":"https:\/\/id.crossref.org\/issn\/0095-1137","issn-type":"print"},{"key":"ref12","first-page":"461","article-title":"Genomic species identification of Acinetobacter of clinical isolates by 16S rDNA sequencing","volume":"46","author":"Misbah S","year":"2005","journal-title":"Singapore Med J","ISSN":"https:\/\/id.crossref.org\/issn\/0037-5675","issn-type":"print"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"2974","DOI":"10.1128\/JCM.01021-06","article-title":"Identification of Acinetobacter baumannii by detection of the blaOXA-51-like carbapenemase gene intrinsic to this species","volume":"58","author":"Turton J","year":"2006","journal-title":"J Clin Microbiol","ISSN":"https:\/\/id.crossref.org\/issn\/0095-1137","issn-type":"print"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"6823","DOI":"10.1093\/nar\/19.24.6823","article-title":"Distribution of repetitive DNA sequences in eubacterial and application to fingerprinting of bacterial genomes","volume":"19","author":"Versalovic J","year":"1991","journal-title":"Nucl Acids Res","ISSN":"https:\/\/id.crossref.org\/issn\/0305-1048","issn-type":"print"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.ijantimicag.2006.01.004","article-title":"Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter sp","volume":"27","author":"Woodford N","year":"2006","journal-title":"Int J Antimicr Agents"},{"key":"ref16","first-page":"1","article-title":"Multiplex PCR for rapid detection of genes encoding acquired metallo-\u00e2-lactamases","volume":"21","author":"Ellington MJ","year":"2006","journal-title":"J Antimicr Chemother"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1590\/S1517-83822009000100013","article-title":"Spread of metallo-\u00e2-lactamases: screening reveals the presence of a blaSPM-1 gene in hospital sewage in Southern Brazil","volume":"40","author":"Fuentefria DB","year":"2009","journal-title":"Braz J Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1093\/jac\/dkp256","article-title":"The changing epidemiology of resistance","volume":"64","author":"Hawkey PM","year":"2009","journal-title":"J Antimicr Chemother"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1089\/mdr.2004.10.286","article-title":"Genotypic characterization of carbapenem-nonsusceptible Acinetobacter sp. isolated in Latin America","volume":"10","author":"Gales AC","year":"2004","journal-title":"Microb Drug Resist","ISSN":"https:\/\/id.crossref.org\/issn\/1076-6294","issn-type":"print"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1089\/mdr.2009.0060","article-title":"Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii in a newly established Greek Hospital","volume":"15","author":"Papa A","year":"2009","journal-title":"Microb Drug Resist","ISSN":"https:\/\/id.crossref.org\/issn\/1076-6294","issn-type":"print"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"22","DOI":"10.3201\/eid1101.040001","article-title":"Multidrug-resistant Acinetobacter baumannii","volume":"11","author":"Abbo A","year":"2005","journal-title":"Emerg Infect Dis","ISSN":"https:\/\/id.crossref.org\/issn\/1080-6040","issn-type":"print"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.ijantimicag.2004.08.013","article-title":"Dissemination and diversity of metallo-\u00e2-lactamases in Latin America: report from the SENTRY Antimicrobial Surveillance Program","volume":"25","author":"Sader HS","year":"2005","journal-title":"Int J Antimicr Agents"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.diagmicrobio.2004.01.002","article-title":"Comparison of the double-disk, combined disk, and Etest methods for detecting metallo-\u00e2-lactamases in gram-negative bacilli","volume":"49","author":"Yan JJ","year":"2004","journal-title":"Diagn Microbiol Infec Dis"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"4174","DOI":"10.1128\/AAC.49.10.4174-4179.2005","article-title":"Characterization of the naturally occurring oxacillinase of Acinetobacter baumannii","volume":"49","author":"Heritier C","year":"2005","journal-title":"Antimicr Agents Chemother"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1128\/CMR.00001-07","article-title":"Carbapenamases: the versatile \u00e2-lactamases","volume":"20","author":"Queenan AM","year":"2007","journal-title":"Clin Microbiol Rev","ISSN":"https:\/\/id.crossref.org\/issn\/0893-8512","issn-type":"print"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1086\/314040","article-title":"Nosocomial bloodstream infections caused by Acinetobacter species in United States Hospitals: clinical features, molecular epidemiology, and antimicrobial susceptibility","volume":"31","author":"Wisplinghoff H","year":"2000","journal-title":"Clin Infect Dis","ISSN":"https:\/\/id.crossref.org\/issn\/1058-4838","issn-type":"print"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1007\/s15010-009-9003-9","article-title":"Carbapenem-resistant Acinetobacter baumannii producing the OXA-23 enzyme: dissemination in Southern Brazil","volume":"37","author":"Martins AF","year":"2009","journal-title":"Infection","ISSN":"https:\/\/id.crossref.org\/issn\/0173-2129","issn-type":"print"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1007\/s10096-010-0878-2","article-title":"Prevalence of antibiotics resistance and OXA carbapenemases genes in multidrug-resistant Acinetobacter baumannii isolates in central Taiwan","volume":"29","author":"Yang SC","year":"2010","journal-title":"Eur J Microbiol Infect Dis"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1093\/jac\/dkp428","article-title":"Global spread of carbapenem-resistant Acinetobacter baumannii","volume":"65","author":"Higgins PG","year":"2010","journal-title":"J Antimicr Chemoth"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1093\/jac\/dkn537","article-title":"Nosocomial spread of OXA-23 and OXA-58 \u00e2-lactamase-producing Acinetobacter baumannii in a Bulgarian hospital","volume":"63","author":"Stoeva T","year":"2009","journal-title":"J Antimicr Chemoth"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1111\/j.1469-0691.2009.02770.x","article-title":"Clonal dissemination of two clusters of Acinetobacter baumannii producing OXA-23 or OXA-58 in Rome, Italy","volume":"15","author":"Mendes RE","year":"2009","journal-title":"Clin Microbiol Infect"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"3403","DOI":"10.1128\/JCM.41.7.3403-3406.2003","article-title":"Outbreak of carbapenem-resistant Acinetobacter baumannii producingthe OXA-23 enzyme in Curitiba, Brazil","volume":"41","author":"Dalla-Costa LM","year":"2003","journal-title":"J Clin Microbiol","ISSN":"https:\/\/id.crossref.org\/issn\/0095-1137","issn-type":"print"},{"key":"ref33","first-page":"423","article-title":"Outbreaks of imipenem-resistant Acinetobacter baumanni producing carbapenemases in Korea","volume":"44","author":"Jeong SH","year":"2006","journal-title":"J Microbiol"}],"container-title":["Revista da Sociedade Brasileira de Medicina Tropical"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rsbmt\/v44n6\/14.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,25]],"date-time":"2021-12-25T11:31:51Z","timestamp":1640431911000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0037-86822011000600014&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12]]},"references-count":33,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2011,12]]}},"alternative-id":["S0037-86822011000600014"],"URL":"https:\/\/doi.org\/10.1590\/s0037-86822011000600014","relation":{},"ISSN":["0037-8682"],"issn-type":[{"value":"0037-8682","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12]]}}}