{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T13:01:04Z","timestamp":1695906064819},"reference-count":29,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Soc. Bras. Med. Trop."],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:p>INTRODUCTION: During histoplasmosis, Histoplasma capsulatum soluble antigens (CFAg) can be naturally released by yeast cells. Because CFAg can be specifically targeted during infection, in the present study we investigated CFAg release in experimental murine histoplasmosis, and evaluated the host humoral immune response against high-molecular-mass antigens (hMMAg. &gt;150 kDa), the more immunogenic CFAg fraction. METHODS: Mice were infected with 2.2x10(4) H. capsulatum IMT\/HC128 yeast cells. The soluble CFAg, IgG anti-CFAg, IgG anti-hMMAg, and IgG-hMMAg circulating immune complexes (CIC) levels were determined by enzymelinked immunosorbent assay, at days 0, 7, 14, and 28 post-infection. RESULTS: We observed a progressive increase in circulating levels of CFAg, IgG anti-CFAg, IgG anti-hMMAg, and IgG-hMMAg CIC after H. capsulatum infection. The hMMAg showed a high percentage of carbohydrates and at least two main immunogenic components. CONCLUSIONS: We verified for the first time that hMMAg from H. capsulatum IMT\/HC128 strain induce humoral immune response and lead to CIC formation during experimental histoplasmosis.<\/jats:p>","DOI":"10.1590\/s0037-86822012000200018","type":"journal-article","created":{"date-parts":[[2012,4,25]],"date-time":"2012-04-25T09:16:06Z","timestamp":1335345366000},"page":"232-237","source":"Crossref","is-referenced-by-count":7,"title":["Carbohydrate-rich high-molecular-mass antigens are strongly recognized during experimental Histoplasma capsulatum infection"],"prefix":"10.1590","volume":"45","author":[{"given":"Fabrine Sales Massafera","family":"Trist\u00e3o","sequence":"first","affiliation":[{"name":"Universidade Estadual de Londrina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula C\u00e9sar","family":"Leonello","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciene Airy","family":"Nagashima","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayako","family":"Sano","sequence":"additional","affiliation":[{"name":"Chiba University, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rio Augusto","family":"Ono","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiko Nakagawa","family":"Itano","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","series-title":"The organism Histoplasma capsulatum: Histoplasmosis","first-page":"12","author":"Schwarz J","year":"1981"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1128\/MMBR.53.2.186-209.1989","article-title":"Dimorphism in Histoplasma capsulatum: a model for the study of cell differentiation in pathogenic fungi","volume":"53","author":"Maresca B","year":"1989","journal-title":"Microbiol Rev","ISSN":"http:\/\/id.crossref.org\/issn\/0146-0749","issn-type":"print"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1128\/CMR.4.4.411","article-title":"Histoplasma variation and adaptive strategies for parasitism: new perspectives on histoplasmosis","volume":"4","author":"Eissenberg LG","year":"1991","journal-title":"Clin Microbiology Rev"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1034\/j.1600-0560.2002.290404.x","article-title":"Disseminated cutaneous histoplasmosis in patients infected with human immunodeficiency virus","volume":"29","author":"Ramdial PK","year":"2002","journal-title":"J Cutan Pathol","ISSN":"http:\/\/id.crossref.org\/issn\/0303-6987","issn-type":"print"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1378\/chest.93.5.916","article-title":"Histoplasmosis in patients at risk for the acquired immunodeficiency syndrome in a nonendemic setting","volume":"93","author":"Salzman SH","year":"1988","journal-title":"Chest","ISSN":"http:\/\/id.crossref.org\/issn\/0012-3692","issn-type":"print"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/S1369-5274(00)00104-1","article-title":"Immune response to early and late Histoplasma capsulatum infections","volume":"3","author":"Deepe GS","year":"2000","journal-title":"Curr Opin Microbiol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1080\/02681219180000061","article-title":"The use of cell-free antigens of Paracoccidioides brasiliensis in serological tests","volume":"29","author":"Camargo ZP","year":"1991","journal-title":"J Med Vet Mycol","ISSN":"http:\/\/id.crossref.org\/issn\/0268-1218","issn-type":"print"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1016\/j.micinf.2004.12.017","article-title":"Efficacy of cell-free antigens in evaluating cell immunity and inducing protection in a murine model of histoplasmosis","volume":"7","author":"Sa-Nunes A","year":"2005","journal-title":"Microbes and infection\/Institut Pasteur"},{"key":"ref9","first-page":"155","article-title":"Evaluation of enzyme linked immunosorbent-assay and western blot for diagnosis of histoplasmosis","volume":"45","author":"Torres M","year":"1993","journal-title":"Rev Invest Clin","ISSN":"http:\/\/id.crossref.org\/issn\/0034-8376","issn-type":"print"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1080\/02681219880000571","article-title":"Serodiagnosis of histoplasmosis, paracoccidioidomycosis and penicilliosis marneffei; current status and future trends","volume":"36","author":"Hamilton AJ","year":"1998","journal-title":"Med Mycol","ISSN":"http:\/\/id.crossref.org\/issn\/1369-3786","issn-type":"print"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"2252","DOI":"10.1128\/JCM.35.9.2252-2255.1997","article-title":"Comparison of radioimmunoassay and enzyme-linked immunoassay methods for detection of Histoplasma capsulatum var. capsulatum antigen","volume":"35","author":"Durkin MM","year":"1997","journal-title":"J Clin Microbiol","ISSN":"http:\/\/id.crossref.org\/issn\/0095-1137","issn-type":"print"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1099\/jmm.0.05469-0","article-title":"ELISA for early diagnosis of histoplasmosis","volume":"53","author":"Guimar\u00e3es AJ","year":"2004","journal-title":"J Med Microbiol","ISSN":"http:\/\/id.crossref.org\/issn\/0022-2615","issn-type":"print"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"2909","DOI":"10.1128\/JCM.38.8.2909-2913.2000","article-title":"Comparison of an established antibody sandwich method with an inhibition method of Histoplasma capsulatum antigen detection","volume":"38","author":"Garringer TO","year":"2000","journal-title":"J Clinical Microbiol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"2354","DOI":"10.1128\/AAC.45.8.2354-2357.2001","article-title":"Clearance of fungal burden during treatment of disseminated histoplasmosis with liposomal amphotericin B versus itraconazole","volume":"45","author":"Wheat LJ","year":"2001","journal-title":"Antimicrob Agents Chemother","ISSN":"http:\/\/id.crossref.org\/issn\/0066-4804","issn-type":"print"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0732-8893(02)00367-X","article-title":"Diagnosis of histoplasmosis by antigen detection based upon experience at the histoplasmosis reference laboratory","volume":"43","author":"Wheat LJ","year":"2002","journal-title":"Diagn Microbiol Infect Dis","ISSN":"http:\/\/id.crossref.org\/issn\/0732-8893","issn-type":"print"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1007\/s11046-009-9243-3","article-title":"Soluble components of Histoplasma capsulatum var. capsulatum have hemagglutinin activity and induce syngeneic hemophagocytosis in vitro","volume":"169","author":"Vivan RH","year":"2010","journal-title":"Mycopathol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry OH","year":"1951","journal-title":"J Biol Chem","ISSN":"http:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1080\/02681219680000461","article-title":"Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis","volume":"34","author":"Unterkircher CS","year":"1996","journal-title":"J Med Vet Mycol","ISSN":"http:\/\/id.crossref.org\/issn\/0268-1218","issn-type":"print"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications","volume":"76","author":"Towbin H","year":"1979","journal-title":"Proc Natl Acad Sci USA","ISSN":"http:\/\/id.crossref.org\/issn\/0027-8424","issn-type":"print"},{"key":"ref20","doi-asserted-by":"crossref","DOI":"10.1038\/168167a0","article-title":"A colorimetric method for the determination of sugars","volume":"168","author":"Dubois M","year":"1951","journal-title":"Nature","ISSN":"http:\/\/id.crossref.org\/issn\/0028-0836","issn-type":"print"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1128\/IAI.61.2.714-718.1993","article-title":"Transfer of protective immunity in murine histoplasmosis by a CD4+ T-cell clone","volume":"61","author":"Allendoerfer R","year":"1993","journal-title":"Infection and Immunity","ISSN":"http:\/\/id.crossref.org\/issn\/0019-9567","issn-type":"print"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1128\/IAI.57.3.896-901.1989","article-title":"Production and characterization of murine monoclonal antibodies to Histoplasma capsulatum yeast cell antigens","volume":"57","author":"Kamel SM","year":"1989","journal-title":"Infection Immunity"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1002\/jcla.20078","article-title":"Reactivity of antibodies from patients with acute and chronic paracoccidioidomycosis to a high molecular mass antigen from Paracoccidioides brasiliensis","volume":"19","author":"Marquez AS","year":"2005","journal-title":"J Clin Lab Anal","ISSN":"http:\/\/id.crossref.org\/issn\/0887-8013","issn-type":"print"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/s11046-007-0095-4","article-title":"Protection induced in BALB\/c mice by the high-molecular-mass (hMM) fraction of Paracoccidioides brasiliensis","volume":"163","author":"Pavanelli WR","year":"2007","journal-title":"Mycopathol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1001\/archinte.1979.03630430076025","article-title":"Histoplasmosis","volume":"139","author":"Bullock W","year":"1979","journal-title":"Arch Intern Med","ISSN":"http:\/\/id.crossref.org\/issn\/0003-9926","issn-type":"print"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1128\/CMR.00027-06","article-title":"Histoplasmosis: a clinical and laboratory update","volume":"20","author":"Kauffman CA","year":"2007","journal-title":"Clin Microbiol Rev","ISSN":"http:\/\/id.crossref.org\/issn\/0893-8512","issn-type":"print"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1128\/CMR.6.2.89","article-title":"Paracoccidioidomycosis: an update","volume":"6","author":"Brummer E","year":"1993","journal-title":"Clin Microbiol Rev","ISSN":"http:\/\/id.crossref.org\/issn\/0893-8512","issn-type":"print"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1002\/art.1780260902","article-title":"Rheumatologic manifestations of histoplasmosis in the recent Indianapolis epidemic","volume":"26","author":"Rosenthal J","year":"1983","journal-title":"Arthritis Rheum","ISSN":"http:\/\/id.crossref.org\/issn\/0004-3591","issn-type":"print"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1080\/13693780310001644734","article-title":"Histoplasma capsulatum yeast cells attach and agglutinate human erythrocytes","volume":"42","author":"Taylor ML","year":"2004","journal-title":"Med Mycol","ISSN":"http:\/\/id.crossref.org\/issn\/1369-3786","issn-type":"print"}],"container-title":["Revista da Sociedade Brasileira de Medicina Tropical"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rsbmt\/v45n2\/v45n2a18.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,14]],"date-time":"2022-01-14T03:50:24Z","timestamp":1642132224000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0037-86822012000200018&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["S0037-86822012000200018"],"URL":"https:\/\/doi.org\/10.1590\/s0037-86822012000200018","relation":{},"ISSN":["0037-8682"],"issn-type":[{"value":"0037-8682","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4]]}}}