{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T19:57:05Z","timestamp":1648583825836},"reference-count":13,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Soc. Bras. Med. Trop."],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:p>INTRODUCTION: The study analyzed positivity of polymerase chain reaction (PCR) on detection of DNA from Leishmania in patients' samples. METHODS: Extracted DNA was submitted to L150\/L152, 13Y\/13Z, and seminested PCR (snPCR). RESULTS: Results were evidenced by bands of approximately 120, 720, and 670 bp for L150\/L152, 13Y\/13Z, and snPCR, respectively. L150\/L152, 13Y\/13Z, and snPCR positivity indexes were 76.9, 56.4, and 9.2 (p&gt;0.05), respectively, for suspected and 93.7, 68.7, and 84.4 (p&lt;0.05), respectively, for confirmed. CONCLUSIONS: Preliminary results showed that these assays, mainly L150\/L152 and snPCR, can detect Leishmania DNA and carry potential on laboratory diagnosis of leishmaniasis.<\/jats:p>","DOI":"10.1590\/s0037-86822012000200023","type":"journal-article","created":{"date-parts":[[2012,4,25]],"date-time":"2012-04-25T09:16:09Z","timestamp":1335345369000},"page":"257-259","source":"Crossref","is-referenced-by-count":4,"title":["Comparison among three polymerase chain reaction assays on detection of DNA from Leishmania in biological samples from patients with american cutaneous leishmaniasis"],"prefix":"10.1590","volume":"45","author":[{"given":"Jo\u00e3o Guilherme Lino da","family":"Silva","sequence":"first","affiliation":[{"name":"Universidade Federal de Alfenas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thiago Miranda da","family":"Silva","sequence":"additional","affiliation":[{"name":"Universidade Estadual de 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