{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T15:16:36Z","timestamp":1773674196038,"version":"3.50.1"},"reference-count":28,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Soc. Bras. Med. Trop."],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:p>INTRODUCTION: The laboratory diagnosis of schistosomiasis is based mainly on the detection of parasite eggs in stool samples through the Kato-Katz (KK) technique, reading one slide by test. However, a widely known limitation of parasitological methods is reduced sensitivity, particularly in low endemic areas. METHODS: To increase sensitivity, we conducted further slide readings from the same stool sample using the parasitological method associated with a serological test. We used the KK method (three slides) and the IgG anti-Schistosoma mansoni-enzyme-linked immunosorbent assay (ELISA) technique to diagnose schistosomiasis in low endemic areas in the Brazilian State of Cear\u00e1. Fecal samples and sera from 250 individuals were analyzed. RESULTS: Sixteen percent and 47.2% of samples were positive in parasitological tests and serological tests, respectively. Parasitological methods showed that 32 (80%) individuals tested positive on the first slide, 6 (15%) on the second slide, and 2 (5%) on the third. The performance of the ELISA test in the diagnosis, using the KK method as diagnostic reference, showed a negative predictive value of 100%, with specificity and positive predictive values of 62.8% and 33.9%, respectively. CONCLUSIONS: In this study, the increase from one to three slides analyzed per sample using the KK technique was shown to be a useful procedure for increasing the diagnostic sensitivity of this technique.<\/jats:p>","DOI":"10.1590\/s0037-86822012000400019","type":"journal-article","created":{"date-parts":[[2012,8,25]],"date-time":"2012-08-25T09:53:39Z","timestamp":1345888419000},"page":"510-513","source":"Crossref","is-referenced-by-count":22,"title":["Increased detection of schistosomiasis with Kato-Katz and SWAP-IgG-ELISA in a Northeastern Brazil low-intensity transmission area"],"prefix":"10.1590","volume":"45","author":[{"given":"Teiliane Rodrigues","family":"Carneiro","sequence":"first","affiliation":[{"name":"Universidade Federal do Cear\u00e1; Universidade Federal do Cear\u00e1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta Cristhiany Cunha","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Universidade Federal do Cear\u00e1; Universidade Federal do Cear\u00e1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara Menezes de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Federal do Cear\u00e1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana L\u00facia de Paula","family":"Hanemann","sequence":"additional","affiliation":[{"name":"Universidade Federal do Cear\u00e1; Universidade Federal do Cear\u00e1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Ajax Nogueira","family":"Queiroz","sequence":"additional","affiliation":[{"name":"Universidade Federal do Cear\u00e1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando Schemelzer Moraes","family":"Bezerra","sequence":"additional","affiliation":[{"name":"Universidade Federal do Cear\u00e1; Universidade Federal do Cear\u00e1; Universidade Federal do 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feces","volume":"4","author":"Gomes LI","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1590\/S0074-02762006000900006","article-title":"Low transmission areas of schistosomiasis in Venezuela: consequences on the diagnosis, treatment, and control","volume":"101","author":"Noya BA","year":"2006","journal-title":"Mem Inst Oswaldo Cruz","ISSN":"https:\/\/id.crossref.org\/issn\/0074-0276","issn-type":"print"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.actatropica.2008.05.012","article-title":"Immuno-epidemiology of Schistosoma mansoni infections in endemic populations co-infected with soil-transmitted helminths: Present knowledge, challenges, and the need for further studies","volume":"108","author":"Geiger SM","year":"2008","journal-title":"Acta 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Hyg","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9637","issn-type":"print"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1590\/S0102-311X2008000200016","article-title":"Utiliza\u00e7\u00e3o de m\u00e9todo sorol\u00f3gico como ferramenta diagn\u00f3stica para implementa\u00e7\u00e3o da vigil\u00e2ncia e controle da esquistossomose no Munic\u00edpio de Holambra, S\u00e3o Paulo, Brasil","volume":"24","author":"Gargioni C","year":"2008","journal-title":"Cad Saude Publica","ISSN":"https:\/\/id.crossref.org\/issn\/0102-311X","issn-type":"print"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1080\/00034983.1998.11813278","article-title":"The distribution of Schistosoma japonicum eggs in faeces and the effect of stirring faecal specimens","volume":"92","author":"Yu XP","year":"1998","journal-title":"Trop Med 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antigens","volume":"38","author":"Corr\u00eaa-Oliveira R","year":"1988","journal-title":"Am J Trop Med Hyg","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9637","issn-type":"print"},{"key":"ref21","first-page":"136","article-title":"The last fifteen years of schistosomiasis in Venezuela: features and evolution","volume":"94","author":"Noya BA","year":"1999","journal-title":"Mem Inst Oswaldo Cruz","ISSN":"https:\/\/id.crossref.org\/issn\/0074-0276","issn-type":"print"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1590\/S0036-46651989000200009","article-title":"Schistosomiasis mansoni in the municipality of Pedro de Toledo (S\u00e3o Paulo, Brazil) where the Biomphalaria tenagophila is the snail host: I. Prevalence in human population","volume":"31","author":"Dias LCS","year":"1989","journal-title":"Rev Inst Med Trop"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/0035-9203(93)90415-M","article-title":"Seroepidemiology and serodiagnosis of schistosomiasis in Kenya using crude and purified egg antigens of Schistosoma mansoni in ELISA","volume":"87","author":"Doenhoff MJ","year":"1993","journal-title":"Trans Roy Soc Trop Med Hyg"},{"key":"ref24","first-page":"52","article-title":"Evaluation of an enzyme linked immunosorbent assay (ELISA) using Schistosoma mansoni soluble egg antigen as a diagnostic tool for Schistosoma mansoni infection in Ethiopian schoolchildren","volume":"95","author":"Eltiro F","year":"1992","journal-title":"Am J Trop Med Hyg","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9637","issn-type":"print"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.3201\/eid1310.061423","article-title":"Epidemiology of schistosomiasis in the People's Republic of China, 2004","volume":"13","author":"Zhou XN","year":"2007","journal-title":"Emerg Infect Dis","ISSN":"https:\/\/id.crossref.org\/issn\/1080-6040","issn-type":"print"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.trstmh.2008.11.013","article-title":"A single FLOTAC is more sensitive than triplicate Kato-Katz for the diagnosis of low intensity soil transmitted helminth infections","volume":"103","author":"Knopp S","year":"2009","journal-title":"Trans R Soc Trop Med Hyg","ISSN":"https:\/\/id.crossref.org\/issn\/0035-9203","issn-type":"print"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"157","DOI":"10.4269\/ajtmh.2002.66.157","article-title":"Detection by polymerase chain reaction of Schistosoma mansoni DNA in human serum and feces","volume":"66","author":"Pontes LA","year":"2002","journal-title":"Am J Trop Med Hygiene"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"652","DOI":"10.4269\/ajtmh.2003.68.652","article-title":"Comparison of a polymerase chain reaction and the Kato-Katz technique for diagnosing infection with Schistosoma mansoni","volume":"68","author":"Pontes LA","year":"2003","journal-title":"Am J Tropic Med Hygiene"}],"container-title":["Revista da Sociedade Brasileira de Medicina Tropical"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rsbmt\/v45n4\/a19v45n4.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,7,3]],"date-time":"2019-07-03T04:12:19Z","timestamp":1562127139000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0037-86822012000400019&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,8]]}},"alternative-id":["S0037-86822012000400019"],"URL":"https:\/\/doi.org\/10.1590\/s0037-86822012000400019","relation":{},"ISSN":["0037-8682"],"issn-type":[{"value":"0037-8682","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8]]}}}