{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T12:46:51Z","timestamp":1648644411664},"reference-count":15,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cir. Bras."],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:p>OBJETIVO: Estudo comparativo do enxerto aut\u00f3logo e heter\u00f3logo de ov\u00e1rio na bolsa omental de ratas, utilizando ciclosporina A. M\u00c9TODOS: No presente estudo, foram utilizadas 20 ratas distribu\u00eddas randomicamente em quatro grupos, denominados grupo controle (animais submetidos \u00e0 ooforectomia); grupo A (enxerto aut\u00f3logo de ov\u00e1rio); grupo H (enxerto heter\u00f3logo de ov\u00e1rio); e grupo C (enxerto heter\u00f3logo de ov\u00e1rio e tratamento com ciclosporina A). Todos os animais foram submetidos \u00e0 ooforectomia bilateral. Um dos ov\u00e1rios removidos foi individualizado e enxertado no omento maior do animal. Os animais do grupo C receberam a dose de 10mg\/kg\/dia de ciclosporina por via subcut\u00e2nea durante todo o per\u00edodo do experimento. Ap\u00f3s um per\u00edodo de 33 dias, todos os animais foram submetidos a uma nova interven\u00e7\u00e3o cir\u00fargica para identifica\u00e7\u00e3o do leito receptor e retirada do ov\u00e1rio transplantado, al\u00e9m do \u00fatero e da vagina, seguido de eutan\u00e1sia. As pe\u00e7as foram submetidas a processamento histol\u00f3gico e coloras pela hematoxilina eosina. RESULTADOS: Todos os grupos apresentaram epit\u00e9lios vaginal e uterino com 1 a 2 fileiras de c\u00e9lulas c\u00fabicas ou cil\u00edndricas simples, n\u00facleo arredondado e central. Houve tamb\u00e9m grande rea\u00e7\u00e3o inflamat\u00f3ria em todo o enxerto, com \u00e1reas de necrose. Ocorreu \u00f3bito de um animal do grupo C no 20\u00ba dia do experimento. CONCLUS\u00c3O: Conclui-se que o enxerto de ov\u00e1rio \u00edntegro realizado em omento maior de ratas nesta amostra, independente do uso da Ciclosporina, n\u00e3o mostrou-se vi\u00e1vel.<\/jats:p>","DOI":"10.1590\/s0102-86502005000200013","type":"journal-article","created":{"date-parts":[[2006,1,10]],"date-time":"2006-01-10T16:12:58Z","timestamp":1136909578000},"page":"174-179","source":"Crossref","is-referenced-by-count":2,"title":["Estudo da viabilidade do enxerto de ov\u00e1rio na bolsa omental de ratos utilizando Ciclosporina A"],"prefix":"10.1590","volume":"20","author":[{"given":"Nara Macedo Botelho","family":"Brito","sequence":"first","affiliation":[{"name":"Universidade do Estado do Par\u00e1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"In\u00eaz Ohashi","family":"Torres","sequence":"additional","affiliation":[{"name":"UEPA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edvaldo Lima","family":"Silveira","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Par\u00e1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorena Lira Tavares dos","family":"Santos","sequence":"additional","affiliation":[{"name":"UEPA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1200\/JCO.1997.15.4.1309","article-title":"High-dose chemotherapy with autologous transplantation for persistent\/relapsed ovarian cancer: a multivariate analysis of survival for 100 consecutively treated patients","volume":"15","author":"Stiff PJ","year":"1997","journal-title":"J Clin Oncol."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1210\/endo.134.3.8119153","article-title":"Immature rat ovaries become revascularized rapidly after autotransplantation and show gonadotropin-dependent increase in angiogenic factor gene expression","volume":"134","author":"Dissen GA","year":"1994","journal-title":"Endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0013-7227","issn-type":"print"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1111\/j.1471-0528.1982.tb04710.x","volume":"89","author":"Wood C","year":"1982","journal-title":"Br J Obstet Gynaecol."},{"key":"ref4","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1590\/S0102-86501998000100007","article-title":"Estudo experimental do enxerto aut\u00f3logo de ov\u00e1rio em cadelas submetidas \u00e0 ovariectomia e ovariosalpingohisterectomia","volume":"13","author":"Matera JM","year":"1998","journal-title":"Acta Cir Bras."},{"key":"ref5","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/S0015-0282(98)00095-8","article-title":"Freezing, thawing, and autograft of ovarian fragments in sheep: preliminary experiments and histologic assessment","volume":"70","author":"Salle B","year":"1998","journal-title":"Fert Ster."},{"key":"ref6","first-page":"28","article-title":"Estudo microsc\u00f3pico de ov\u00e1rio autotransplantado em ratas submetidas \u00e0 ooforectomia","volume":"16","author":"Brito NMB","year":"2002","journal-title":"Rev Paul Med."},{"issue":"2","key":"ref7","doi-asserted-by":"crossref","first-page":"551","DOI":"10.3181\/00379727-123-31540","article-title":"Restablishment of ovarian periodicity after transplantation to the syrian hamster cheek pouch","volume":"123","author":"Caldwell BV","year":"1966","journal-title":"Proc Soc Exp Biol Med."},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1590\/S0100-72032002000300007","article-title":"Implante aut\u00f3logo ovariano no omento maior: Estudo experimental","volume":"24","author":"Alberti LR","year":"2002","journal-title":"Rev Bras Ginecol Obstet."},{"issue":"2","key":"ref9","first-page":"64","article-title":"Estudo comparativo da viabilidade de fragmento de tecido espl\u00eanico aut\u00f3logo implantado no omento maior ou na tela sub-cut\u00e2nea de ratas","volume":"6","author":"Rodrigues AZ","year":"1991","journal-title":"Acta Cir Bras."},{"key":"ref10","first-page":"16","article-title":"Subcutaneous autologous ovarian transplantation in Wistar rat maintains hormone secretion","volume":"70","author":"Corleta HE","year":"1998","journal-title":"Fert. 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