{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T15:04:33Z","timestamp":1718723073846},"reference-count":25,"publisher":"FapUNIFESP (SciELO)","issue":"suppl 4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cir. Bras."],"published-print":{"date-parts":[[2006]]},"abstract":"<jats:p>PURPOSE: To evaluate the effects of intraluminal injection of glutamine on the serum trolox equivalent antioxidant capacity in an experimental model of ischemia-reperfusion of the liver observing the applicability of modifications on the original assay method. METHODS: Thirty Wistar rats underwent laparotomy to perform a 20 cm blind sac of small bowel and occlusion of the hepatic hilo for 30 minutes and reperfusion for 5 minutes. Into the gut sac it was injected glutamine (glutamine group, n=10) or distilled water (control group, n=10). Ten other animals (sham group) underwent laparotomy without artery occlusion. Blood samples were collected for trolox equivalent antioxidant capacity assays in different temperature conditions, reagent quantities and time for spectrophotometer readings. RESULTS: Total antioxidant capacity was significantly greater in glutamine group than in both control group (1,60[1,55-1,77] vs 1,44[1,27-1,53]) and sham group (1,60[1,55-1,77] vs 1,48[1,45-1,59]). CONCLUSION: Glutamine enhanced serum antioxidant capacity. The assay technique consistently reflected the changes in the antioxidant defenses in this experimental model.<\/jats:p>","DOI":"10.1590\/s0102-86502006001000015","type":"journal-article","created":{"date-parts":[[2007,3,15]],"date-time":"2007-03-15T12:44:05Z","timestamp":1173962645000},"page":"69-73","source":"Crossref","is-referenced-by-count":4,"title":["Intestinal intraluminal injection of glutamine increases trolox total equivalent antioxidant capacity (TEAC) in hepatic ischemia-reperfusion"],"prefix":"10.1590","volume":"21","author":[{"given":"Alberto Bicudo","family":"Salom\u00e3o","sequence":"first","affiliation":[{"name":"Federal University of Mato Grosso, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Eduardo","family":"Aguilar-Nascimento","sequence":"additional","affiliation":[{"name":"Federal University of Mato Grosso, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandro","family":"Perc\u00e1rio","sequence":"additional","affiliation":[{"name":"Federal University of Para, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor","family":"Sano","sequence":"additional","affiliation":[{"name":"Federal University of Mato Grosso, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole Ribeiro","family":"Marques","sequence":"additional","affiliation":[{"name":"Federal University of Mato Grosso, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia Cristina Gomes de Oliveira","family":"Dias","sequence":"additional","affiliation":[{"name":"Federal University of Mato Grosso, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"issue":"70","key":"ref1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1093\/bmb\/ldh025","article-title":"Vascular ischaemia and reperfusion injury","volume":"19","author":"Eltzschig HK","year":"2004","journal-title":"Br Med Bull."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1097\/00000658-190810000-00005","article-title":"Notes on the arrest of hepatic hemorrhage due to trauma","volume":"48","author":"Pringle JH","year":"1908","journal-title":"Ann Surg."},{"issue":"3","key":"ref3","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1590\/S0102-86502002000900020","article-title":"Aspectos b\u00e1sicos da les\u00e3o de isquemia e reperfus\u00e3o e do pr\u00e9-condicionamento isqu\u00eamico","volume":"17","author":"Silva Jr.","year":"2002","journal-title":"Acta Cir Bras."},{"key":"ref4","first-page":"47","article-title":"Ischemia-reperfusion injury: role of oxygen-derived free radicals","volume":"548","author":"Granger DN","year":"1986","journal-title":"Acta Physol Scand Suppl."},{"issue":"1","key":"ref5","first-page":"18","article-title":"Oxidative stress and total antioxidant status in ischemic skin flaps in rats","volume":"19","author":"Cymrot M","year":"2004","journal-title":"Acta Cir. Bras."},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1001\/archsurg.1991.01410250112019","article-title":"Oxygen-derived free radicals","volume":"126","author":"Hinder RA","year":"1991","journal-title":"Arch Surg."},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1016\/S0002-8223(21)00865-8","article-title":"Intestinal fuels: glutamine, short-chain fatty acids, and dietary fiber","volume":"92","author":"Evans MA","year":"1992","journal-title":"J. Am. Diet. Assoc."},{"key":"ref8","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1177\/0148607185009005608","article-title":"Glutamine metabolism by the intestinal tract","volume":"9","author":"Souba WW","year":"1985","journal-title":"JPEN","ISSN":"http:\/\/id.crossref.org\/issn\/0148-6071","issn-type":"print"},{"issue":"18","key":"ref9","doi-asserted-by":"crossref","first-page":"7185","DOI":"10.1073\/pnas.87.18.7185","article-title":"High-affinity transport of glutathione is part of a multicomponent system essential for mitochondrial function","volume":"87","author":"Martensson J","year":"1990","journal-title":"Proc Natl Acad Sci USA."},{"key":"ref10","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1159\/000070606","article-title":"Benefits of intraluminal injection of glutamine for intestinal mucosa during ischemia-reperfusion","volume":"4","author":"Aguilar-Nascimento JE","year":"2003","journal-title":"Eur Surg Res."},{"key":"ref11","series-title":"Free radicals, Oxidative stress and drug action","first-page":"169","volume-title":"Antioxidant status measured by the TRAP assay in rheumathoid arthrits","author":"Thumham DI","year":"1987"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/0003-2670(92)85052-8","article-title":"Enhanced chemiluminescent assay for antioxidant capacity in biologic fluids","volume":"266","author":"Writehead TP","year":"1992","journal-title":"Analytica Chimca Acta."},{"issue":"6","key":"ref13","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1093\/clinchem\/44.6.1309","article-title":"Camparasion of different analytical methods for assessing total antioxidant capacity of humam serum","volume":"44","author":"Cao G","year":"1998","journal-title":"Clinical Chemistry","ISSN":"http:\/\/id.crossref.org\/issn\/0009-9147","issn-type":"print"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0014-5793(85)81208-4","article-title":"Quantitative measurement of the total peroxyl radicaltrapping antioxidant capacity of humam blody plasma by controlled peroxidation","volume":"187","author":"Wayne DDM","year":"1985","journal-title":"FEBS lett."},{"key":"ref15","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/0076-6879(90)86106-6","article-title":"Phycoerythrin fluorescence-based assay for reactive oxygen species","volume":"186","author":"Glazer AN","year":"1990","journal-title":"Methods Enzymol","ISSN":"http:\/\/id.crossref.org\/issn\/0076-6879","issn-type":"print"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/0891-5849(94)00102-P","article-title":"A fluorescence-based method for measuring total plasma antioxidant capacity","volume":"18","author":"Ghiseli A","year":"1994","journal-title":"Free Rad Biol Med."},{"key":"ref17","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1042\/cs0840407","article-title":"A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates","volume":"84","author":"Miller N","year":"1993","journal-title":"Clin Sci."},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1738","DOI":"10.1093\/clinchem\/41.12.1738","article-title":"Automated oxygen radical absorbance capacity assay using COBAS FARA II","volume":"41","author":"Cao G","year":"1995","journal-title":"Clin Chem."},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0891-5849(98)00315-3","article-title":"Antioxidant activity applying an improved ABTS radical cation decolorization assay","volume":"26","author":"Re R","year":"1999","journal-title":"Free Rad Biol Med."},{"key":"ref20","first-page":"175","article-title":"The reaction between ABTS radical c\u00e1tion and antioxidants and its use to evaluet the antioxidant status of serum samples","volume":"29","author":"Romay C","year":"1996","journal-title":"Braz J Med Biol Res","ISSN":"http:\/\/id.crossref.org\/issn\/0100-879X","issn-type":"print"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1080\/13510002.1996.11747044","article-title":"Spectrophtometric determination of antioxidant activity","volume":"2","author":"Miller N","year":"1996","journal-title":"Redox Rpt"},{"issue":"2","key":"ref22","doi-asserted-by":"crossref","first-page":"51","DOI":"10.4067\/S0716-97602001000200009","article-title":"Methodologies for evaluation of total antioxidant activities in complex mixtures: A critical review","volume":"34","author":"Llesuy S","year":"2001","journal-title":"Biol. Res."},{"key":"ref23","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/0076-6879(94)34095-1","article-title":"Total antioxidant status in plasma and body fluids","volume":"234","author":"Rice-Evans C","year":"1994","journal-title":"Methods Enzymol.","ISSN":"http:\/\/id.crossref.org\/issn\/0076-6879","issn-type":"print"},{"key":"ref24","first-page":"59","article-title":"Measurement of total antioxidant capacity as a marker of antioxidant status in vivo: procedures and limitations","volume":"33","author":"Rice-Evans CA","year":"2000","journal-title":"Free Rad Res."},{"key":"ref25","series-title":"Altera\u00e7\u00f5es oxidativas e da defesa antioxidante no broncoespasmo agudo induzido em cobaias","author":"Perc\u00e1rio S.","year":"2000"}],"container-title":["Acta Cirurgica Brasileira"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/acb\/v21s4\/v21s4a15.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T02:38:54Z","timestamp":1707705534000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0102-86502006001000015&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006]]},"references-count":25,"journal-issue":{"issue":"suppl 4","published-print":{"date-parts":[[2006]]}},"alternative-id":["S0102-86502006001000015"],"URL":"https:\/\/doi.org\/10.1590\/s0102-86502006001000015","relation":{},"ISSN":["0102-8650"],"issn-type":[{"value":"0102-8650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006]]}}}