{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T09:21:35Z","timestamp":1770888095568,"version":"3.50.1"},"reference-count":16,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cir. Bras."],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:p>PURPOSE: To evaluate an experimental model for anorectal anomalies and their principal associated malformations induced by ethylene thiourea (ETU). METHODS: Rat fetuses were utilized, divided into two groups: experimental group - fetuses from rats that received ETU on the 11th day of gestation at the dose of 125 mg\/kg, diluted in distilled water to 1% concentration (12.5 ml\/kg); and control group - fetuses from rats that received distilled water alone, at a volume of 12.5 ml\/kg. On the 21st day of gestation, the animals were sacrificed by hypoxia in a carbon dioxide chamber, followed by laparotomy to remove the fetuses. These were initially examined externally to determine the sex and whether anorectal anomalies and malformations of the vertebral column and tail were present. Then, with the aid of microscopy, the fetuses underwent exploratory laparotomy to characterize the type of anorectal anomaly and investigate urological malformations. RESULTS: None of the fetuses in the control group presented anorectal anomaly, vertebral column malformation or urological structural alterations. In the experimental group, 71% presented anorectal anomaly, 80% presented vertebral column alterations and 35% presented urological alterations. CONCLUSION: The model described was shown to be easy to implement and presented results that allow its use in studying anorectal anomalies and associated malformations.<\/jats:p>","DOI":"10.1590\/s0102-86502007000200010","type":"journal-article","created":{"date-parts":[[2007,3,26]],"date-time":"2007-03-26T15:04:55Z","timestamp":1174921495000},"page":"130-136","source":"Crossref","is-referenced-by-count":14,"title":["Evaluation of an experimental model for anorectal anomalies induced by ethylenethiourea"],"prefix":"10.1590","volume":"22","author":[{"given":"Maur\u00edcio","family":"Macedo","sequence":"first","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Luiz","family":"Martins","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karine Furtado","family":"Meyer","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","series-title":"Ano-rectal malformations in children","author":"Stephens FD","year":"1971"},{"key":"ref2","series-title":"Cirurgia pedi\u00e1trica","first-page":"809","volume-title":"Anomalias anorretais","author":"Pe\u00f1a A","year":"1998"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1111\/j.1464-410X.2004.05053.x","article-title":"Sacral Ratio and fecal continence in children with anorectal malformations","volume":"94","author":"Macedo M","year":"2004","journal-title":"BJU Int"},{"issue":"9","key":"ref4","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1053\/jpsu.2002.34996","article-title":"Clarification of the process that lead to anorectal malformations in the ETU-induced rat model of imperforate anus","volume":"37","author":"Qui BQ","year":"2002","journal-title":"J Pediatr Surg","ISSN":"https:\/\/id.crossref.org\/issn\/0022-3468","issn-type":"print"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1002\/aja.1000450204","article-title":"Developmental anomalies in a special strin of mice","volume":"45","author":"Danforth CH","year":"1930","journal-title":"Am J Anat","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9106","issn-type":"print"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/S0002-9440(10)61747-6","article-title":"Anorectal Malformations caused by defects in sonic hedgehog signaling","volume":"159","author":"Mo R","year":"2001","journal-title":"Am J Pathol","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9440","issn-type":"print"},{"issue":"12","key":"ref7","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1016\/S0022-3468(87)80728-5","article-title":"The internal sphincter in anorectal malformation: Morphologic investigations in neonatal pigs","volume":"22","author":"Lambrecht W","year":"1987","journal-title":"J Pediatr Surg","ISSN":"https:\/\/id.crossref.org\/issn\/0022-3468","issn-type":"print"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.1053\/jpsu.2001.26373","article-title":"Mapping loci causing susceptibility to anal atresia in pigs, using a resource pedigree","volume":"36","author":"Hori T","year":"2001","journal-title":"J Pediatr Surg","ISSN":"https:\/\/id.crossref.org\/issn\/0022-3468","issn-type":"print"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1055\/s-2008-1072368","article-title":"Congenital anomalies in mice induced by etretinate","volume":"10","author":"Kubota Y","year":"2000","journal-title":"Eur J Pediatr Surg","ISSN":"https:\/\/id.crossref.org\/issn\/0939-7248","issn-type":"print"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1002\/tera.1420070305","article-title":"Ethylenethiourea: Teratogenicity study in rats and rabbits","volume":"7","author":"Khera KS","year":"1973","journal-title":"Teratology","ISSN":"https:\/\/id.crossref.org\/issn\/0040-3709","issn-type":"print"},{"issue":"12","key":"ref11","first-page":"277","article-title":"Pattern of anomalies following single oral doses of ethylenethiourea to pregnant rats","volume":"1975","author":"Ruddick JA","journal-title":"Teratology","ISSN":"https:\/\/id.crossref.org\/issn\/0040-3709","issn-type":"print"},{"issue":"7","key":"ref12","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1016\/S0022-3468(05)80028-4","article-title":"Transplacentally induced anorectal malformations in rats","volume":"25","author":"Hirai Y","year":"1990","journal-title":"J Pediatr Surg."},{"issue":"9","key":"ref13","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1053\/jpsu.2002.34996","article-title":"Clarification of the process that lead to anorectal malformations in the ETU-induced rat model of imperforate anus","volume":"37","author":"Qui BQ","year":"2002","journal-title":"J Pediatr Surg","ISSN":"https:\/\/id.crossref.org\/issn\/0022-3468","issn-type":"print"},{"issue":"9","key":"ref14","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1016\/S0022-3468(03)00387-7","article-title":"Evidence that the notochord may be pivotal in the development of sacral and anorectal malformations","volume":"38","author":"Qui BQ","year":"2003","journal-title":"J Pediatr Surg","ISSN":"https:\/\/id.crossref.org\/issn\/0022-3468","issn-type":"print"},{"issue":"9","key":"ref15","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1016\/S0022-3468(03)00401-9","article-title":"Deficient motor innervation of the sphincter mechanism in fetal rats with anorectal malformation: a quantitative study by fluorogold retrograde tracing","volume":"38","author":"Yuan ZW","year":"2003","journal-title":"J Pediatr Surg","ISSN":"https:\/\/id.crossref.org\/issn\/0022-3468","issn-type":"print"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1053\/jpsu.2001.20721","article-title":"Impaired distribution of retinoic acid receptors in the hindgut-tailgut region of murine embryos with anorectal malformations","volume":"36","author":"Bitoh Y","year":"2001","journal-title":"J Pediatr Surg","ISSN":"https:\/\/id.crossref.org\/issn\/0022-3468","issn-type":"print"}],"container-title":["Acta Cirurgica Brasileira"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/acb\/v22n2\/10.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,5,25]],"date-time":"2017-05-25T14:02:24Z","timestamp":1495720944000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0102-86502007000200010&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4]]},"references-count":16,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2007,4]]}},"alternative-id":["S0102-86502007000200010"],"URL":"https:\/\/doi.org\/10.1590\/s0102-86502007000200010","relation":{},"ISSN":["0102-8650"],"issn-type":[{"value":"0102-8650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4]]}}}