{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T08:15:30Z","timestamp":1759565730895},"reference-count":18,"publisher":"FapUNIFESP (SciELO)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cir. Bras."],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:p>PURPOSE: To produce a myelomeningocele-like human defect in the ovine fetus and validate this experimental model in our population. METHODS: A prospective study on 12 pregnant sheep of a crossed Hampshire\/Down breed where a spinal defect was surgically created between Day 75 and Day 77 after conception. The technique consisted of a hysterotomy with exposure of fetal hind limbs and tail up to the mid spine. Fetal skin, paravertebral muscles, and 4 posterior spinal arches were excised, exposing the spinal cord. Duramater was opened and the medulla was incised until the medullar canal. Animals were euthanized at 139 days of gestation for fetal evaluation. The central nervous system was submitted to post-mortem magnetic resonance imaging (MRI) and the spine was submitted to pathological examination. RESULTS: The defect was created in 13 fetuses and 5 survived. Mean gestational age at necropsy was 121.6 days (varying from 93 to 145 days). Macroscopically, the defect was present in 4 cases. Microscopy revealed a flattened medulla with disappearance of the medullar canal and disruption of normal medullar architecture with neuronal apoptosis and\/or fusion of the piamater and duramater. The MRI showed herniation of the cerebellum into the cervical canal and syringomyelia. CONCLUSIONS: The surgically produced defect mimics the defect found in the human fetus, including the Arnold-Chiari malformation. Post-mortem MRI was used for the first time in our study and proved an excellent alternative for demonstrating the cerebellar herniation. We standardized the technique for creating the defect in our population.<\/jats:p>","DOI":"10.1590\/s0102-86502007000300003","type":"journal-article","created":{"date-parts":[[2007,9,27]],"date-time":"2007-09-27T22:57:00Z","timestamp":1190933820000},"page":"168-173","source":"Crossref","is-referenced-by-count":20,"title":["Validation of the ovine fetus as an experimental model for the human myelomeningocele defect"],"prefix":"10.1590","volume":"22","author":[{"given":"Denise Ara\u00fajo Lapa","family":"Pedreira","sequence":"first","affiliation":[{"name":"Albert Einstein Hospital,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita de C\u00e1ssia Sanchez e","family":"Oliveira","sequence":"additional","affiliation":[{"name":"USP,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Roberto","family":"Valente","sequence":"additional","affiliation":[{"name":"Dante Pazzanese Institute of Cardiology,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rog\u00e9rio C.","family":"Abou-Jamra","sequence":"additional","affiliation":[{"name":"USP,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrezza","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Dante Pazzanese Institute of Cardiology,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Hil\u00e1rio","family":"Saldiva","sequence":"additional","affiliation":[{"name":"USP,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","article-title":"Fetal surgery for MMC and the incidence of shunt-dependent hydrocephalus","volume":"1819-25","author":"Bruner JP","year":"1999","journal-title":"J Am Med Assoc"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1826","DOI":"10.1001\/jama.282.19.1826","article-title":"Improvement in hindbrain herniation demonstrated by serial fetal MRI following fetal surgery for myelomeningocele","volume":"282","author":"Sutton LN","year":"1999","journal-title":"J Am Med Assoc"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1067\/mob.2000.108867","article-title":"Hindbrain herniation develops in surgically created myelomeningocele but is absent after repair in fetal lambs","volume":"183","author":"Paek BW","year":"2000","journal-title":"Am J Obstet Gynecol","ISSN":"http:\/\/id.crossref.org\/issn\/0002-9378","issn-type":"print"},{"key":"ref4","first-page":"259","article-title":"Intrauterine treatment of spina bifida: primate model","volume":"39","author":"Michejda M","year":"1984","journal-title":"Z Kinderchir","ISSN":"http:\/\/id.crossref.org\/issn\/0174-3082","issn-type":"print"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1227\/00006123-199006000-00011","article-title":"The paralysis associated with myelomeningocele: clinical and experimental data implicating a preventable spinal cord injury","volume":"26","author":"Heffez DS","year":"1990","journal-title":"Neurosurgery","ISSN":"http:\/\/id.crossref.org\/issn\/0148-396X","issn-type":"print"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1016\/S0022-3468(97)90603-5","article-title":"The spinal cord lesion in human fetuses with myelomeningocele: implications for fetal surgery","volume":"32","author":"Meuli M","year":"1997","journal-title":"J Pediatr Surg","ISSN":"http:\/\/id.crossref.org\/issn\/0022-3468","issn-type":"print"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/S0022-3468(96)90746-0","article-title":"In utero repair of experimental myelomeningocele saves neurological function at birth","volume":"31","author":"Meuli M","year":"1996","journal-title":"J Pediatr Surg","ISSN":"http:\/\/id.crossref.org\/issn\/0022-3468","issn-type":"print"},{"issue":"6","key":"ref8","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1159\/000065388","article-title":"A different technique to create a 'myelomeningocele-like' defect in the fetal rabbit","volume":"17","author":"Pedreira DA","year":"2002","journal-title":"Fetal Diagn Ther","ISSN":"http:\/\/id.crossref.org\/issn\/1015-3837","issn-type":"print"},{"issue":"2","key":"ref9","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1590\/S0102-86502003000200009","article-title":"Estudo de uma nova t\u00e9cnica para cria\u00e7\u00e3o cir\u00fargica de um defeito semelhante a meningomielocele em fetos de coelhos","volume":"18","author":"Pedreira DA","year":"2003","journal-title":"Acta Cir. 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