{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T12:38:44Z","timestamp":1762605524467},"reference-count":27,"publisher":"FapUNIFESP (SciELO)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cir. Bras."],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:p>PURPOSE: To develop an experimental model of severe acute pancreatitis in rabbits through a pancreatic ductal injection of sodium taurocholate. METHODS: Twenty-four albino rabbits of the New Zealand lineage were distributed into four groups of six animals (A, B, C and S). The rabbits of three experimental groups (A, B and C) were submitted to a laparatomy and received a pancreatic ductal injection of 1ml\/kg sodium taurocholate 5%. Also, they were submitted to further laparatomies after 4h, 8h and 12h, respectively. The control group (S) was subdivided into two groups of three animals: in subgroup S1 only the pancreatic duct catheterization was performed whereas in subgroup S2 the pancreatic duct catheterization as well as an injection of 1ml\/kg physiologic solution 0.9% were carried out. After 12 hours, the rabbits were evaluated. In the re-intervention, blood was collected to determine the amylasemia and a pancreatectomy was carried out to investigate interstitial infiltration, steatonecrosis and necrosis of the organ, using an optical microscope. RESULTS: There was an elevation of amylase in all groups thus proving the existence of acute pancreatitis. The size of the interlobular septum increased progressively with a greater variation between group S1 (0.13) and group C (0. 53) (p=0.035). While all the animals in group A exhibited focal cellular necrosis, it was more intense in the rabbits of group B and culminated with a high proportion of severe pancreatic necrosis in group C animals. The difference in the intensity of cellular necrosis showed statistic significance (p=0.001). CONCLUSION: The proposed experimental model demonstrated its reproducibility and effectiveness in producing severe acute pancreatitis in rabbits.<\/jats:p>","DOI":"10.1590\/s0102-86502007000500008","type":"journal-article","created":{"date-parts":[[2007,10,4]],"date-time":"2007-10-04T19:47:02Z","timestamp":1191527222000},"page":"366-371","source":"Crossref","is-referenced-by-count":6,"title":["Experimental model of severe acute pancreatitis in rabbits"],"prefix":"10.1590","volume":"22","author":[{"given":"Alberto","family":"Goldenberg","sequence":"first","affiliation":[{"name":"UNIFESP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Celia Diniz Cabral Barbosa","family":"Romeo","sequence":"additional","affiliation":[{"name":"UNIFESP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rcia Bento","family":"Moreira","sequence":"additional","affiliation":[{"name":"UNIBAN; Cruzeiro do Sul University, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franz Robert","family":"Apodaca","sequence":"additional","affiliation":[{"name":"UNIFESP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo Moura","family":"Linhares","sequence":"additional","affiliation":[{"name":"UNIFESP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques","family":"Matone","sequence":"additional","affiliation":[{"name":"UNIFESP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1001\/archsurg.1993.01420170122019","article-title":"A clinically based classification system for acute pancreatitis","volume":"128","author":"Bradley EL III","year":"1993","journal-title":"Arch Surg."},{"issue":"3","key":"ref2","first-page":"377","article-title":"Practice guidelines in acute pancreatitis","volume":"92","author":"Banks PA","year":"1997","journal-title":"Am J Gastroenterol."},{"key":"ref3","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1148\/radiol.2233010680","article-title":"Acute pancreatitis: assessment of severity with clinical and CT evaluation","volume":"223","author":"Balthazar EJ","year":"2002","journal-title":"Radiology","ISSN":"http:\/\/id.crossref.org\/issn\/0033-8419","issn-type":"print"},{"issue":"4","key":"ref4","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1097\/00004836-200006000-00002","article-title":"Etiology and pathogenesis of acute pancreatitis","volume":"30","author":"Sakorafas GH","year":"2000","journal-title":"J Clin Gastroenterol."},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1056\/NEJM199404283301706","article-title":"Acute pancreatitis","volume":"330","author":"Steinberg W","year":"1994","journal-title":"N Engl J Med."},{"key":"ref6","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1016\/j.gtc.2004.07.009","article-title":"Early events in acute pancreatitis","volume":"33","author":"Halangk W","year":"2004","journal-title":"Gastroenterol Clin N Am."},{"key":"ref7","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1097\/00000658-199201000-00007","article-title":"A better model of acute pancreatitis for evaluate therapy","volume":"215","author":"Schmidt J","year":"1992","journal-title":"Ann Surg."},{"key":"ref8","author":"Lerch MM","year":"2004"},{"issue":"3","key":"ref9","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1097\/00006676-199504000-00014","article-title":"Activation of Trypsinogen in experimental models of acute pancreatitis in rats","volume":"10","author":"Nakae Y","year":"1995","journal-title":"Pancreas","ISSN":"http:\/\/id.crossref.org\/issn\/0885-3177","issn-type":"print"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1136\/gut.43.2.232","article-title":"A monoclonal anti-interleukin 8 antibody (WS-4) inhibits cytokine response and acute lung injury in experimental severe acute necrotizing pancreatitis in rabbits","volume":"43","author":"Osman M","year":"1998","journal-title":"Gut","ISSN":"http:\/\/id.crossref.org\/issn\/0017-5749","issn-type":"print"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1006\/cyto.2001.0977","article-title":"Profiles of pro-inflammatory cytokines in the serum of rabbits after experimentally induced acute pancreatitis","volume":"17","author":"Osman MO","year":"2002","journal-title":"Cytokine","ISSN":"http:\/\/id.crossref.org\/issn\/1043-4666","issn-type":"print"},{"issue":"6","key":"ref12","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1159\/000018774","article-title":"Protein C activation during the initial phase of experimental acute pancreatitis in the rabbit","volume":"16","author":"Ottesen LH","year":"1999","journal-title":"Dig Surg."},{"issue":"14","key":"ref13","doi-asserted-by":"crossref","first-page":"2082","DOI":"10.3748\/wjg.v10.i14.2082","article-title":"Effects of octreotide on acute necrotizing pancreatitis in rabbits","volume":"10","author":"Czako L","year":"2004","journal-title":"World J Gastroenterol."},{"key":"ref14","first-page":"477","article-title":"Protective effect of a protein-free diet in acute experimental pancreatitis","volume":"22","author":"Gon\u00e7alez Y","year":"1989","journal-title":"Braz J Med Biol Res."},{"issue":"3","key":"ref15","first-page":"285","article-title":"Mononuclear phagocytic system in acute pancreatitis","volume":"26","author":"Abdo EE","year":"1993","journal-title":"Braz J Med Biol Res."},{"issue":"4","key":"ref16","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/S1091-255X(01)80063-9","article-title":"Effect of platelet-activating factor antagonists (BN-52021, WEB-2170, and BB-882) on bacterial translocation in acute pancreatitis","volume":"5","author":"de Souza LJ","year":"2001","journal-title":"J Gastrointest Surg."},{"issue":"2","key":"ref17","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1159\/000070086","article-title":"Effects of octreotide pretreatment in experimental acute pancreatitis","volume":"3","author":"Salem MZ","year":"2003","journal-title":"Pancreatology"},{"issue":"1","key":"ref18","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1097\/01.mpa.0000191645.01926.8f","article-title":"Local and systemic effects of hypertonic solution (NaCl 7,5%) in experimental acute pancreatitis","volume":"32","author":"Machado MC","year":"2006","journal-title":"Pancreas","ISSN":"http:\/\/id.crossref.org\/issn\/0885-3177","issn-type":"print"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1002\/bjs.1800810805","article-title":"Experimental models of acute pancreatitis","volume":"81","author":"Banerjee A.K.","year":"1994","journal-title":"Br J Surg."},{"key":"ref20","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/BF02888986","article-title":"Pancreatic injury caused by intraductal injection of foreign serum in the rat","volume":"27","author":"Nevalainen T.J.","year":"1978","journal-title":"Virchows Arch B Cell Pathol."},{"key":"ref21","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1007\/BF00224474","article-title":"Studies on intracellular transport of secretory proteins in the rat exocrine pancreas: IV Stimulation by in vivo infusion of caerulein","volume":"165","author":"Bieger W","year":"1976","journal-title":"Cell Tissue Res."},{"issue":"3","key":"ref22","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1097\/00006676-199504000-00014","article-title":"Activation of Trypsinogen in experimental models of acute pancreatitis in rats","volume":"10","author":"Nakae Y","year":"1995","journal-title":"Pancreas","ISSN":"http:\/\/id.crossref.org\/issn\/0885-3177","issn-type":"print"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1016\/0016-5085(93)91022-A","article-title":"Pancreatic duct obstruction triggers acute necrotizing pancreatitis in the opossum","volume":"104","author":"Lerch MM","year":"1993","journal-title":"Gastroenterology","ISSN":"http:\/\/id.crossref.org\/issn\/0016-5085","issn-type":"print"},{"issue":"6","key":"ref24","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1111\/j.1740-8261.1998.tb01651.x","article-title":"Endoscopic ultrasonographic findings of the pancreas after pancreatic duct ligation in the dog","volume":"39","author":"Morita Y","year":"1998","journal-title":"Vet .Radiol Ultrasound."},{"key":"ref25","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1097\/00006676-199003000-00011","article-title":"Injection of microspheres into pancreatic arteries causes acute haemorrhagic pancreatitis in the rat: a new animal model","volume":"5","author":"Redha F","year":"1990","journal-title":"Pancreas","ISSN":"http:\/\/id.crossref.org\/issn\/0885-3177","issn-type":"print"},{"key":"ref26","first-page":"277","article-title":"Acute pancreatitis after microembolism of the rat pancreas progresses into chronic pancreatitis","volume":"54","author":"Freiburghaus AU","year":"1993","journal-title":"Digestion","ISSN":"http:\/\/id.crossref.org\/issn\/0012-2823","issn-type":"print"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/379333a0","article-title":"Phylogenetic position of the order Lagomorpha (rabbits, hares and allies)","volume":"379","author":"Graur D","year":"1996","journal-title":"Nature","ISSN":"http:\/\/id.crossref.org\/issn\/0028-0836","issn-type":"print"}],"container-title":["Acta Cirurgica Brasileira"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/acb\/v22n5\/08.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,14]],"date-time":"2023-05-14T09:53:47Z","timestamp":1684058027000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0102-86502007000500008&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,10]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["S0102-86502007000500008"],"URL":"https:\/\/doi.org\/10.1590\/s0102-86502007000500008","relation":{},"ISSN":["0102-8650"],"issn-type":[{"value":"0102-8650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,10]]}}}