{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:35:44Z","timestamp":1761510944787},"reference-count":24,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cir. Bras."],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:p>PURPOSE: To evaluate the protective role of dimethylsulfoxide (DMSO) in a rat model of testis ischemia\/reperfusion (I\/R). METHODS: Twenty-four male Wistar rats were randomized in two equal groups. Control rats (G-1) received saline 2.0 ml intraperitoneally (ip) 21, 9 and 1 h before torsion. Experimental rats (G-2) received ip injections of 3% aqueous solution of DMSO, 0.1ml\/10g body weight. Saline was added to complete 2.0ml when necessary. I\/R injury was induced in anesthetized rats by torsion of the right testis lasting 3 hours. Testis and blood samples were collected at the end of ischemia (T-0) and 3 hours later (T-3) for assessment of testis malonaldehyde (MDA), reduced glutathione (GSH), and plasma total antioxidant power (TAP). RESULTS: MDA levels decreased significantly in G-2 rats compared with G-1 animals in all time-points. GSH levels increased significantly in T-0 and T-3 time-points in DMSO pretreated rats compared with G-1 rats. GSH levels increased significantly during reperfusion in G-2 rats. TAP was similar in both groups denoting absence of systemic effects in this study. CONCLUSION: Pretreatment with DMSO reduces testis lipid peroxidation and oxidative stress caused by torsion\/detorsion of the testis.<\/jats:p>","DOI":"10.1590\/s0102-86502010000400011","type":"journal-article","created":{"date-parts":[[2010,7,28]],"date-time":"2010-07-28T23:53:50Z","timestamp":1280361230000},"page":"357-361","source":"Crossref","is-referenced-by-count":19,"title":["Dimethylsulfoxide attenuates ischemia-reperfusion injury in rat testis"],"prefix":"10.1590","volume":"25","author":[{"given":"Sergio Botelho","family":"Guimar\u00e3es","sequence":"first","affiliation":[{"name":"UFC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu Sandes","family":"Kimura","sequence":"additional","affiliation":[{"name":"UFC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Roberto Leit\u00e3o de","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"UFC, 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metabolites","volume":"161","author":"Reilly PM","year":"1991","journal-title":"Am J Surg","ISSN":"http:\/\/id.crossref.org\/issn\/0002-9610","issn-type":"print"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1001\/archsurg.136.10.1201","article-title":"Antioxidants in critical illness","volume":"136","author":"Bulger EM","year":"2001","journal-title":"Arch Surg","ISSN":"http:\/\/id.crossref.org\/issn\/0004-0010","issn-type":"print"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/s00240-004-0436-4","article-title":"Raxofelast, a hydrophilic vitamin e-like antioxidant, reduces testicular ischemia-reperfusion injury","volume":"32","author":"Romeo C","year":"2004","journal-title":"Urol Res","ISSN":"http:\/\/id.crossref.org\/issn\/0300-5623","issn-type":"print"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/S0006-2952(03)00002-9","article-title":"Multidisciplinary utilization of dimethyl sulfoxide: 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Ellman's reagent","volume":"24","author":"Sedlak J","year":"1968","journal-title":"Anal Biochem","ISSN":"http:\/\/id.crossref.org\/issn\/0003-2697","issn-type":"print"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1095\/biolreprod49.2.401","article-title":"Spermatic cord torsion: loss of spermatogenesis despite return blood flow","volume":"49","author":"Turner TT","year":"1993","journal-title":"Biol Reprod","ISSN":"http:\/\/id.crossref.org\/issn\/0006-3363","issn-type":"print"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1152\/physrev.1994.74.1.139","article-title":"Cellular defenses against damage from reactive oxygen species","volume":"74","author":"Yu BP","year":"1994","journal-title":"Physiol Rev","ISSN":"http:\/\/id.crossref.org\/issn\/0031-9333","issn-type":"print"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1016\/j.urology.2005.06.101","article-title":"Effect of morphine on 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