{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T23:20:53Z","timestamp":1696288853562},"reference-count":29,"publisher":"FapUNIFESP (SciELO)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cir. Bras."],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:p>PURPOSE: To investigate the effects of cirrhosis on colonic anastomosis healing in rats. METHODS: Fifty five Wistar male rats were used (23 in the control group and 32 in the cirrhosis group). On the first day of the procedure, the rats in the cirrhosis group underwent double ligation and folding of the common bile duct to induce liver cirrhosis, and the control rats underwent a laparotomy and intestinal manipulation. On the fourteenth and thirty-fifth days, all of the animals were biochemically assessed for serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase, bilirubin, total protein, and albumin and for liver histopathology. On the thirty-fifth day, cirrhosis was confirmed. On the twenty-eighth day, all of the animals were subjected to left colon transection and anastomosis. On the seventh day after the colonic anastomosis, the rats were sacrificed and macroscopically evaluated for dehiscence. The region of the colonic anastomosis was removed and subjected to hydroxyproline content measurement, conventional histology, and the immunohistochemical determination of vascular endothelial growth factor (VEGF) and matrix metalloproteinase type 1 (MMP 1). RESULTS: The biochemical and histopathological examinations confirmed cirrhosis in all of the animals in the cirrhosis group. More deaths occurred after anastomosis in the cirrhosis group (5\/25) than in the control group (0\/21), and anastomotic dehiscence was more frequent in the cirrhosis group (8\/25) than in the control group (0\/21). The average hydroxyproline concentration was lower in the cirrhosis group than in the control group. The immunohistochemical studies showed that the average VEGF expression in the cirrhosis group was lower than in the control group, and the average MMP1 expression was higher in the cirrhosis group. CONCLUSION: Hepatic cirrhosis leads to increased mortality and colonic anastomotic dehiscence, an increased distance between the mucosal edges of the anastomosis area, and a lower hydroxyproline concentration in the colonic anastomosis; therefore, these conditions are deleterious to healing.<\/jats:p>","DOI":"10.1590\/s0102-86502011000600002","type":"journal-article","created":{"date-parts":[[2011,10,29]],"date-time":"2011-10-29T04:01:18Z","timestamp":1319860878000},"page":"415-420","source":"Crossref","is-referenced-by-count":3,"title":["Liver cirrhosis on the colonic anastomotic healing in rats"],"prefix":"10.1590","volume":"26","author":[{"given":"Marcelo","family":"di Bonif\u00e1cio","sequence":"first","affiliation":[{"name":"USP,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rog\u00e9rio Serafim","family":"Parra","sequence":"additional","affiliation":[{"name":"USP,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Luiza Normanha Ribeiro de","family":"Almeida","sequence":"additional","affiliation":[{"name":"USP,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Joaquim Ribeiro da","family":"Rocha","sequence":"additional","affiliation":[{"name":"USP,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omar","family":"Feres","sequence":"additional","affiliation":[{"name":"USP,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"issue":"5","key":"ref1","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1097\/00000658-197305000-00002","article-title":"Factors contributing to leakage of colonic anastomoses","volume":"177","author":"Schrock TR","year":"1973","journal-title":"Ann Surg."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1016\/S0039-6109(16)38296-2","article-title":"Complications of colonic surgery","volume":"47","author":"Bearhs OH.","year":"1967","journal-title":"Surg Clin North Am."},{"key":"ref3","first-page":"747","article-title":"A critical review of colectomy with anastomosis","volume":"135","author":"Debas HT","year":"1972","journal-title":"Surg Ginecol Obstet."},{"key":"ref4","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1002\/bjs.1800570208","article-title":"Anastomostic dehiscence after anterior resection of rectum and sigmoid","volume":"57","author":"Goligher JC","year":"1970","journal-title":"Br J Surg."},{"issue":"5","key":"ref5","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1007\/s10350-004-6627-9","article-title":"Risk factors for anastomotic leakage after left-sided colorectal resection with rectal anastomosis","volume":"46","author":"Makela JT","year":"2003","journal-title":"Dis Colon Rectum."},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1007\/BF02234213","article-title":"Preoperative combined radiotherapy and chemotherapy for rectal cancer does not affect early postoperative morbidity and mortality in low anterior resection","volume":"42","author":"Pucciarelli S","year":"1999","journal-title":"Dis Colon Rectum."},{"issue":"11","key":"ref7","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1007\/BF02554851","article-title":"The importance of intraluminal anastomotic fecal contact and peritonitis in colonic anastomotic leakages: An experimental study","volume":"31","author":"Ravo B","year":"1988","journal-title":"Dis Colon Rectum."},{"key":"ref8","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1590\/S0102-86502008000700005","article-title":"Effects of preoperative pelvic irradiation on colonic anastomosis healing: An experimental study in rats","volume":"23","author":"Franca A","year":"2008","journal-title":"Acta Cir Bras"},{"issue":"4","key":"ref9","first-page":"259","article-title":"A clinic-endoscopic study on healing of lesions and prevention of recurrences in patients with liver cirrhosis and peptic ulcer","volume":"71","author":"Di Mario F","year":"1990","journal-title":"G Clin Med."},{"issue":"11","key":"ref10","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1002\/bjs.1800661113","article-title":"The effect of uraemia upon wound healing","volume":"66","author":"Colin JF","year":"1979","journal-title":"Br J Surg."},{"key":"ref11","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1002\/bjs.1800670204","article-title":"The effect of hipovolaemia on colonic blood flow in dog","volume":"67","author":"Gilmour DG","year":"1980","journal-title":"Br J Surg."},{"issue":"2","key":"ref12","first-page":"79","article-title":"Importance dehydration in anastomotic and subcutaneous wound healing: an experimental study in rats","volume":"158","author":"Hartmann M","year":"1992","journal-title":"Eur J Surg."},{"issue":"6","key":"ref13","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1002\/bjs.1800600612","article-title":"Aetiology of disruption of intestinal anastomoses","volume":"60","author":"Irvin TT","year":"1973","journal-title":"Br J Surg."},{"key":"ref14","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1007\/BF02555426","article-title":"Tissue oxygen tension as a predictor of colonic anastomotic healing","volume":"30","author":"Sheridan WG","year":"1987","journal-title":"Dis Colon Rectum."},{"key":"ref15","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1002\/bjs.1800630515","article-title":"Jaundice and wound healing: an experimental study","volume":"63","author":"Bayer I","year":"1976","journal-title":"Br J Surg."},{"key":"ref16","series-title":"Diseases of the liver","first-page":"725","volume-title":"Cirrhosis","author":"Conn HO","year":"1987"},{"key":"ref17","first-page":"1706","article-title":"Experimental study of the wound healing in liver cirrhosis","volume":"90","author":"Makishima T.","year":"1989","journal-title":"Nippon Geka Gakkai Zasshi","ISSN":"http:\/\/id.crossref.org\/issn\/0301-4894","issn-type":"print"},{"key":"ref18","first-page":"144","article-title":"The effect of immunological response on wound healing in rat with liver cirrhosis","volume":"92","author":"Makishima T","year":"1991","journal-title":"Nippon Geka Gakkai Zasshi","ISSN":"http:\/\/id.crossref.org\/issn\/0301-4894","issn-type":"print"},{"issue":"1","key":"ref19","article-title":"Collagen-stimulating factors in healing wounds and experimental liver disease","volume":"109","author":"McGee JO","year":"1973","journal-title":"J Pathol."},{"issue":"2","key":"ref20","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1590\/S0102-86502001000200003","article-title":"A new method for the experimental induction of secundary biliary cirrhosis in Wistar rats","volume":"16","author":"Jorge GD","year":"2001","journal-title":"Acta Cir Bras."},{"issue":"2","key":"ref21","first-page":"93","article-title":"Menagement of colorectal cancer in patients with cirrhosis and Le Veen shunt","volume":"72","author":"Le Rolland B","year":"1987","journal-title":"Int Surg."},{"key":"ref22","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1007\/BF02554783","article-title":"The surgical risk of colectomy in patients with cirrhosis","volume":"30","author":"Metcalf AM","year":"1987","journal-title":"Dis Colon Rectum."},{"key":"ref23","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1097\/00129689-200106000-00008","article-title":"A comparison of an open and laparoscopic appendectomy for patients with liver cirrhosis","volume":"11","author":"Tsugawa K","year":"2001","journal-title":"Surg Laparosc Endosc Percutan Tech."},{"key":"ref24","series-title":"Modelos experimentais de pesquisa em cirurgia","first-page":"193","volume-title":"Obstru\u00e7\u00e3o biliar extra hep\u00e1tica","author":"Castro e Silva Jr. O","year":"1998"},{"key":"ref25","first-page":"443","article-title":"Healing of ischemic colonic anastomoses in the rat: role of antibiotic preparation","volume":"97","author":"Cohen SR","year":"1985","journal-title":"Surgery","ISSN":"http:\/\/id.crossref.org\/issn\/0039-6060","issn-type":"print"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/0009-8981(67)90167-2","article-title":"Determination of hydroxyproline","volume":"18","author":"Stegemann H","year":"1967","journal-title":"Clin Chim Acta."},{"key":"ref27","series-title":"Nonparametric statistics for the behavioral sciences","author":"Siegel S.","year":"1956"},{"issue":"4","key":"ref28","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1001\/archsurg.1973.01350160185032","article-title":"Abdominal wound dehiscence","volume":"106","author":"Keill RH","year":"1973","journal-title":"Arch Surg."},{"key":"ref29","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1002\/bjs.1800660708","article-title":"Does obstructive jaundice adversely affect wound healing?","volume":"66","author":"Greaney MG","year":"1979","journal-title":"Br J Surg."}],"container-title":["Acta Cirurgica Brasileira"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/acb\/v26n6\/02.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,5,27]],"date-time":"2017-05-27T01:46:41Z","timestamp":1495849601000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0102-86502011000600002&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12]]},"references-count":29,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2011,12]]}},"alternative-id":["S0102-86502011000600002"],"URL":"https:\/\/doi.org\/10.1590\/s0102-86502011000600002","relation":{},"ISSN":["0102-8650"],"issn-type":[{"value":"0102-8650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12]]}}}