{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T16:38:49Z","timestamp":1700239129205},"reference-count":32,"publisher":"FapUNIFESP (SciELO)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cir. Bras."],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:p>PURPOSE: To morphometrically quantify CD1a+ dentritic cells and DC-SIGN+ dendritic cells in HIV-positive patients with anal squamous intraepithelial neoplasia and to evaluate the effects of HIV infection, antiretroviral therapy and HPV infection on epithelial and subepithelial dendritic cells. METHODS: A prospective study was performed to morphometrically analyze the relative volume of the dendritic cells and the relationship between anal intraepithelial neoplasia and cancer in HIV-positive patients from the Tropical Medicine Foundation of Amazonas, Brazil. All patients were submitted to biopsies of anorectal mucosa to perform a classic histopathological and immunohistochemical analysis, employing antibodies against CD1a and DC-SIGN for the morphometric quantification of dendritic cells. RESULTS: HIV-negative patients displayed a CD1a DC density significantly higher than that of HIV-positives patients (3.75 versus 2.54) (p=0.018), and in patients with severe anal intraepithelial neoplasia had correlated between DC CD1a density with levels of CD4 + cells (p: 0.04) as well as the viral load of HIV-1 (p: 0.035). A not significant rise in the median density of CD1a+ DC was observed in the HIV positive\/ HAART positive subgroup compared to the HIV positive\/ HAART negative subgroup. The CD1a+ DC were also significantly increased in HIV-negative patients with anorectal condyloma (2.33 to 3.53; p=0.05), with an opposite effect in HIV-positive patients. CONCLUSIONS: Our data support an enhancement of the synergistic action caused by HIV-HPV co-infection on the anal epithelium, weakening the DC for its major role in immune surveillance. Notoriously in patients with severe anal intraepithelial neoplasia, the density of CD1a+ epithelial dendritic cells was influenced by the viral load of HIV-1. Our study describes for the first time the density of subepithelial DC-SIGN+ dendritic cells in patients with anal severe anal intraepithelial neoplasia and points to the possibility that a specific therapy for HIV induces the recovery of the density of epithelial DC.<\/jats:p>","DOI":"10.1590\/s0102-86502011000600019","type":"journal-article","created":{"date-parts":[[2011,10,29]],"date-time":"2011-10-29T04:01:34Z","timestamp":1319860894000},"page":"521-529","source":"Crossref","is-referenced-by-count":3,"title":["Morphometric analysis of dendritic cells from anal mucosa of HIV-positive patients and the relation to intraepithelial lesions and cancer seen at a tertiary health institution in Brazil"],"prefix":"10.1590","volume":"26","author":[{"given":"Adriana Gon\u00e7alves Daumas Pinheiro","family":"Guimar\u00e3es","sequence":"first","affiliation":[{"name":"UFAM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto Moreira da","family":"Silva Junior","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oscar Tadeu Ferreira da","family":"Costa","sequence":"additional","affiliation":[{"name":"UFAM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan Tramujas da Costa e","family":"Silva","sequence":"additional","affiliation":[{"name":"UFAM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felicidad Santos","family":"Gimenez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Ribamar de","family":"Araujo","sequence":"additional","affiliation":[{"name":"UEA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosilene Viana de","family":"Andrade","sequence":"additional","affiliation":[{"name":"UEA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erico Jander da Silva","family":"Lopes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqueline Pereira","family":"Pinheiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junia Raquel D.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"UFAM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Malheiro","sequence":"additional","affiliation":[{"name":"UFAM"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Carlos de Lima","family":"Ferreira","sequence":"additional","affiliation":[{"name":"UFAM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1097\/QAD.0b013e3283023f78","article-title":"Marked increase in the incidence of invasive anal cancer among HIV: infected patients despite treatment with 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