{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T08:04:58Z","timestamp":1769846698864,"version":"3.49.0"},"reference-count":24,"publisher":"FapUNIFESP (SciELO)","issue":"suppl 1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cir. Bras."],"published-print":{"date-parts":[[2011]]},"abstract":"<jats:p>PURPOSE: To investigate the effect of alanyl-glutamine dipeptide (L-Ala-Gln) pre-treatment on ischemia-reperfusion (I\/R) injury after unilateral testicular torsion-detorsion in a comparative controlled experiment. METHODS: Forty-eight rats (150-200 g) randomly distributed into 4 groups (n=12), and distributed in 2 subgroups (n=6) each, were treated with saline 2.0 ml (G-1, G-3) or L-Ala-Gln 20%, 0.75g\/kg dissolved in saline (total volume 2.0 ml) administered in the left saphenous vein 30 minutes before ischemia. Anesthetized rats were subjected to I\/R induced by torsion (720\u00b0) of the right spermatic cord lasting 1h (G-1, G-2) or 3 hours (G-3, G4). Anesthesia was again applied at the end of ischemia time (T-0) for testis detorsion and 6 hours later (T-6) for orchiectomy. All operations were performed on the right testes through transverse scrotal incisions. Right orchiectomy was carried out at the end of ischemia (T-0), and 6 hours later (T-6) to evaluate the concentrations of malondialdehyde (MDA) and reduced glutathione (GSH) in the testis. RESULTS: Pretreatment with L-Ala-Gln reduced MDA contents in rat testis at the end of ischemia lasting 3 hours. There was significant increase of GSH levels in T-6 time-point after 1 hour of ischemia. GSH levels also increased in T-0 and T-6 time-points in rats subjected to ischemia for 3 hours. CONCLUSION: L-Ala-Gln administered before torsion\/detorsion of the spermatic cord decreases lipid peroxidation during ischemia and protects the testis from oxidative stress by upregulating GSH levels during reperfusion.<\/jats:p>","DOI":"10.1590\/s0102-86502011000700005","type":"journal-article","created":{"date-parts":[[2011,9,29]],"date-time":"2011-09-29T19:21:08Z","timestamp":1317324068000},"page":"21-25","source":"Crossref","is-referenced-by-count":8,"title":["L-Alanyl-Glutamine dipeptide pretreatment attenuates ischemia-reperfusion injury in rat testis"],"prefix":"10.1590","volume":"26","author":[{"given":"Jo\u00e3o Paulo de Vasconcelos","family":"Leit\u00e3o","sequence":"first","affiliation":[{"name":"UFC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jefferson Menezes Viana","family":"Santos","sequence":"additional","affiliation":[{"name":"UFC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raquel Cavalcante 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Am."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/BF00300074","article-title":"Reperfusion injury following testicular torsion and detorsion in prepubertal rats","volume":"21(6)","author":"Blank ML","year":"1993","journal-title":"Urol Res."},{"key":"ref3","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.nut.2006.09.005","article-title":"Protective effect of alpha-lipoic acid in experimental spermatic cord torsion","volume":"23(1)","author":"Guimar\u00e3es SB","year":"2007","journal-title":"Nutrition."},{"key":"ref4","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/S0306-3623(96)00474-0","article-title":"The pharmacology of the antioxidant lipoic acid","volume":"29(3)","author":"Biewenga GP","year":"1997","journal-title":"Gen Pharmacol."},{"key":"ref5","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/s00240-004-0436-4","article-title":"Raxofelast, a hydrophilic vitamin E-like antioxidant, reduces testicular ischemia-reperfusion injury","volume":"32(5)","author":"Romeo C","year":"2004","journal-title":"Urol Res."},{"key":"ref6","first-page":"E443","article-title":"Estimation of glucose-alanine-lactate-glutamine cycles in postabsorptive humans: role of skeletal muscle","volume":"269(3 Pt 1)","author":"Perriello G","year":"1995","journal-title":"Am J Physiol."},{"key":"ref7","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0378-4347(93)80048-9","article-title":"Determination of glutamine and alpha-ketoglutarate concentration and specific activity in plasma using high-performance liquid chromatography","volume":"620(1)","author":"Darmaun D","year":"1993","journal-title":"J Chromatogr."},{"key":"ref8","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1042\/cs0760643","article-title":"Availability of amino acid supplied by constant intravenous infusion of synthetic dipeptide in healthy man","volume":"76(6)","author":"Albers S","year":"1989","journal-title":"Clin Sci."},{"key":"ref9","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0003-2697(78)90342-1","article-title":"Determination of malonaldehyde precursor in tissues by thiobarbituric acid test","volume":"86(1)","author":"Uchiyama M","year":"1978","journal-title":"Anal Biochem."},{"key":"ref10","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/0003-2697(68)90092-4","article-title":"Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent","volume":"25(1)","author":"Sedlak J","year":"1968","journal-title":"Anal Biochem."},{"key":"ref11","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1046\/j.1365-2605.2000.00255.x","article-title":"Torsion-induced injury in rat testes does not affect mitochondrial respiration or the accumulation of mitochondrial mutations","volume":"23(6)","author":"Ahmed FA","year":"2000","journal-title":"Int J Androl."},{"key":"ref12","first-page":"663","article-title":"Correlation of angiographic and histologic studies with systemic arteriosclerosis","volume":"109(7)","author":"Regadera J","year":"1985","journal-title":"Arch Pathol Lab Med."},{"key":"ref13","first-page":"159","article-title":"Oxygen-derived free radicals in postischemic tissue injury","volume":"312(3)","author":"McCord JM","year":"1985","journal-title":"N Engl J Med."},{"key":"ref14","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/s00345-008-0323-7","article-title":"Protective effect of trapidil on long-term histologic damage in a rat model of testicular ischemia-reperfusion injury","volume":"27(1)","author":"Bozlu M","year":"2009","journal-title":"World J Urol."},{"key":"ref15","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1007\/s11255-009-9633-y","article-title":"Rosuvastatin protects tissue perfusion in the experimental testicular torsion model","volume":"42(2)","author":"Karakaya E","year":"2010","journal-title":"Int Urol Nephrol."},{"key":"ref16","first-page":"374","article-title":"Vardenafil reduces testicular damage following ischemia\/reperfusion injury in rats","volume":"25(7)","author":"Erol B","year":"2009","journal-title":"Med Sci."},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1016\/S0022-5347(17)35351-X","article-title":"The effect of allopurinol pretreatment before detorting testicular torsion","volume":"151(6)","author":"Akg\u00fcr FM","year":"1994","journal-title":"J Urol."},{"key":"ref18","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/BF00300074","article-title":"Reperfusion injury following testicular torsion and detorsion in prepubertal rats","volume":"21(6)","author":"Blank ML","year":"1993","journal-title":"Urol Res."},{"key":"ref19","first-page":"199","article-title":"Spermatic cord torsion, reactive oxygen and nitrogen species and ischemia-reperfusion injury","volume":"25(1-2)","author":"Wilhelm Filho D","year":"2004","journal-title":"Mol Aspects Med."},{"key":"ref20","first-page":"271","article-title":"Curcumin attenuates ischemia-reperfusion injury in rat testis","volume":"91(1)","author":"Wei SM","year":"2009","journal-title":"Fertil Steril."},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1095\/biolreprod33.5.1197","article-title":"Estimation of glutathione in purified populations of mouse testis germ cells","volume":"33(5)","author":"Grosshans K","year":"1985","journal-title":"Biol Reprod."},{"key":"ref22","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1146\/annurev.bi.52.070183.003431","article-title":"Glutathione","volume":"52","author":"Meister A","year":"1983","journal-title":"Annu Rev Biochem."},{"key":"ref23","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1146\/annurev.pa.30.040190.003131","article-title":"Glutathione: toxicological implications","volume":"30","author":"Reed DJ","year":"1990","journal-title":"Annu Rev Pharmacol 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