{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T10:03:11Z","timestamp":1791021791302,"version":"4.1.0"},"reference-count":15,"publisher":"FapUNIFESP (SciELO)","issue":"suppl 2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cir. Bras."],"published-print":{"date-parts":[[2011]]},"abstract":"<jats:p>PURPOSE: To study the functional behavior of the allograft with immunosuppression of pancreatic islets in the spleen. METHODS: Five groups of 10 Mongrel dogs were used: Group A (control) underwent biochemical tests; Group B underwent total pancreatectomy; Group C underwent total pancreatectomy and pancreatic islet autotransplant in the spleen; Group D underwent pancreatic islet allograft in the spleen without immunosuppressive therapy; Group E underwent pancreatic islet allograft in the spleen and immunosuppression with cyclosporine. All of the animals with grafts received pancreatic islets prepared by the mechanical-enzymatic method - stationary collagenase digestion and purification with dextran discontinuous density gradient, implanted in the spleen. RESULTS: The animals with autotransplant and those with allografts with immunosuppression that became normoglycemic showed altered results of intravenous tolerance glucose (p &lt; 0.001) and peripheral and splenic vein plasmatic insulin levels were significantly lower (p &lt; 0.001) in animals that had allografts with immunosuppression than in those with just autotransplants. CONCLUSIONS: In the animals with immunosupression with cyclosporine subjected to allograft of pancreatic islets prepared with the mechanical-enzymatic preparation method (stationary collagenase digestion and purification with dextran discontinuous density gradient), the production of insulin is decreased and the response to intravenous glucose is altered.<\/jats:p>","DOI":"10.1590\/s0102-86502011000800011","type":"journal-article","created":{"date-parts":[[2011,10,26]],"date-time":"2011-10-26T01:19:51Z","timestamp":1319591991000},"page":"57-65","source":"Crossref","is-referenced-by-count":4,"title":["Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs"],"prefix":"10.1590","volume":"26","author":[{"given":"Jaques","family":"Waisberg","sequence":"first","affiliation":[{"name":"Faculty of Medicine of ABC,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles Benjamin","family":"Neff","sequence":"additional","affiliation":[{"name":"Faculty of Medicine of ABC,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Reis","family":"Waisberg","sequence":"additional","affiliation":[{"name":"University of Sao Paulo,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demetrius","family":"Germini","sequence":"additional","affiliation":[{"name":"Hospital do Servidor Publico Estadual,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Eduardo","family":"Gon\u00e7alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaldo","family":"Zanotto","sequence":"additional","affiliation":[{"name":"USP,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manlio Basilio","family":"Speranzini","sequence":"additional","affiliation":[{"name":"Faculty of Medicine of ABC,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.transproceed.2006.10.014","article-title":"CITR Investigators. 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Estudo experimental em c\u00e3es","volume":"2","author":"Waisberg J","year":"1987","journal-title":"Acta Cir Bras."},{"key":"ref9","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1590\/S0102-86502002000200002","article-title":"Pancreatic islet isolation by mechanical-enzymatic separation, stationary collagenase digestion and dextran discontinuous density gradient purification: experimental study in dogs","volume":"17","author":"Waisberg J","year":"2002","journal-title":"Acta Cir Bras."},{"key":"ref10","first-page":"51","article-title":"A CIOMS ethical code for animal experimentation","volume":"39","author":"Howard-Jones N","year":"1985","journal-title":"WHO Chron."},{"key":"ref11","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/0022-4804(84)90184-7","article-title":"Total pancreatectomy in dogs","volume":"37","author":"Cobb LF","year":"1984","journal-title":"J Surg Res."},{"key":"ref12","doi-asserted-by":"crossref","first-page":"455","DOI":"10.2337\/diab.30.5.455","article-title":"Preparation of viable islet cells from dogs by a new method","volume":"30","author":"Horaguchi A","year":"1981","journal-title":"Diabetes."},{"key":"ref13","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1097\/TP.0b013e31817ef6c9","article-title":"Improvement of canine islet yield by donor pancreas infusion with a p38MAPK inhibitor","volume":"86","author":"Ito T","year":"2008","journal-title":"Transplantation."},{"key":"ref14","first-page":"14","article-title":"Technical basis of autotransplantation of islets in the spleen","volume":"4","author":"Waisberg J","year":"1989","journal-title":"ABCD Arq Bras Cir Dig."},{"key":"ref15","first-page":"628","article-title":"The pancreas as an islet transplantation site. Confirmation in a syngeneic rodent and canine autotransplant model","volume":"8","author":"Stagner JI","year":"2007","journal-title":"JOP."}],"container-title":["Acta Cirurgica Brasileira"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/acb\/v26s2\/a11v26s2.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,5,26]],"date-time":"2017-05-26T21:09:39Z","timestamp":1495832979000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0102-86502011000800011&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011]]},"references-count":15,"journal-issue":{"issue":"suppl 2","published-print":{"date-parts":[[2011]]}},"alternative-id":["S0102-86502011000800011"],"URL":"https:\/\/doi.org\/10.1590\/s0102-86502011000800011","relation":{},"ISSN":["0102-8650"],"issn-type":[{"value":"0102-8650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011]]}}}