{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T04:13:38Z","timestamp":1741925618761,"version":"3.38.0"},"reference-count":14,"publisher":"FapUNIFESP (SciELO)","issue":"suppl 2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cir. Bras."],"published-print":{"date-parts":[[2011]]},"abstract":"<jats:p>PURPOSE: To assess in vitro the correlation between the number of neurons and the sensitivity to cholinergic drugs and acetylcholinesterase activity in chagasic patients. METHODS: A 3x1 cm strip of the muscle layer of the anterior part of the stomach, always close to the angular incisure, was removed from 10 chronic chagasic patients (6 men) submitted to megaesophagus or megacolon surgery and from 10 non-chagasic patients (4 men) submitted to other types of surgery (control group), aged on average 52.3 and 50.1 years, respectively, for histological and pharmacological studies. The action of cholinergic drugs was investigated in isolated preparations according to the superfusion method of Ferreira and Costa, and acetylcholinesterase activity was determined by the method of Ellman. For neuron count, the strips were cut into 8 \u00b5m sections according to the method standardized by Alc\u00e2ntara. RESULTS: There was a difference in number of neurons between the chagasic (5,6) and control (7,3) groups. Acetylcholinesterase activity, in moles of hydrolyzed substrate per minute per gram tissue, was reduced in chagasic patients (4,32) compared to the controls (7,30). No hypersensitivity of the gastric musculature to cholinergic drugs was detected, with a reduced maximum response to carbachol and betanechol in the chagasic group. CONCLUSIONS: The reduction of neurons in the myenteric plexus of the stomach of chronic chagasic patients can be demonstrated even in the absence of clinical chagasic gastropathy. The hypersensitivity of the gastric musculature to cholinergic drugs probably depends on intense denervation. The reduced acetylcholinesterase activity demonstrates the involvement of the cholinergic innervation in the stomach of chronic chagasic patients. There was no correlation between number of neurons, sensitivity to cholinergic drugs and acetylcholinesterase activity in the gastric musculature of chagasic and non-chagasic patients.<\/jats:p>","DOI":"10.1590\/s0102-86502011000800014","type":"journal-article","created":{"date-parts":[[2011,10,26]],"date-time":"2011-10-26T05:19:31Z","timestamp":1319606371000},"page":"74-78","source":"Crossref","is-referenced-by-count":3,"title":["Histological, biochemical and pharmacologycal characterization of the gastric muscular layer in Chagas disease"],"prefix":"10.1590","volume":"26","author":[{"given":"Wagner","family":"Carlucci","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reginaldo","family":"Ceneviva","sequence":"additional","affiliation":[{"name":"USP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e9rgio Henrique","family":"Ferreira","sequence":"additional","affiliation":[{"name":"USP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orlando Castro de","family":"Silva","sequence":"additional","affiliation":[{"name":"USP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","first-page":"101","article-title":"Histopahologia e pathogenese do megaesophago e megareto","volume":"8","author":"Amorim M","year":"1932","journal-title":"An Fac Med S\u00e3o Paulo."},{"key":"ref2","first-page":"643","article-title":"Etiologia e patogenia do megaes\u00f4fago no Brasil","volume":"47","author":"K\u00f6berle F","year":"1955","journal-title":"Rev Paul Med."},{"key":"ref3","first-page":"155","article-title":"Patog\u00eania da mol\u00e9stia de Chagas","volume":"3","author":"K\u00f6berle F","year":"1957","journal-title":"Rev Goiana Med."},{"key":"ref4","first-page":"5","article-title":"Patologia da mol\u00e9stia de Chagas","author":"K\u00f6berle F","year":"1972","journal-title":"Medicina. Rev. do CARL e do HCFMRP-USP"},{"key":"ref5","first-page":"7","article-title":"Gastrointestinal peptides role in pathophysiology and disease","volume":"77","author":"Creutzfeld W","year":"1982","journal-title":"Scand J Gastroenterol."},{"key":"ref6","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/S0031-6997(25)06930-3","article-title":"Purinergic nerves","volume":"24","author":"Burnstock G","year":"1972","journal-title":"Pharmacol Rev."},{"key":"ref7","first-page":"43","article-title":"Peptidergic nerves","volume":"71","author":"Bishop AE","year":"1982","journal-title":"Scand J Gastroenterol."},{"key":"ref8","first-page":"151","article-title":"Hiperatividade da musculatura g\u00e1strica \u00e0 meticolina na mol\u00e9stia de Chagas","volume":"65","author":"Vieira CB","year":"1964","journal-title":"Hospital."},{"key":"ref9","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1159\/000198709","article-title":"Acid and pepsin secretion in chronic Chagas disease patients in response to graded doses of pentagastrin and pentagastrin plus betanecol","volume":"23","author":"Padovan W","year":"1982","journal-title":"Digestion."},{"key":"ref10","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/0014-2999(76)90147-3","article-title":"A laminar flow superfusion technique with much increased sensitivity for the detection of smooth muscle stimulating substances","volume":"39","author":"Ferreira SH","year":"1976","journal-title":"Eur J Pharmacol."},{"key":"ref11","first-page":"560","article-title":"Desnerva\u00e7\u00e3o dos plexos intramurais do trato digestivo em \"Rattus novergicus albinus\" chag\u00e1sicos cr\u00f4nicos","volume":"21","author":"Alc\u00e2ntara FG","year":"1964","journal-title":"Rev Bras Med."},{"key":"ref12","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/0006-2952(61)90145-9","article-title":"A new and rapid calorimetric determination of acetylcholinesterase activity","volume":"7","author":"Ellman GL","year":"1961","journal-title":"Biochem Pharmacol."},{"key":"ref13","first-page":"1013","article-title":"Mol\u00e9stia de Chagas experimental. Estudo quantitativo de neur\u00f4nios simp\u00e1ticos e parassimp\u00e1ticos","volume":"61","author":"Brand\u00e3o HJS","year":"1962","journal-title":"Hospital."},{"key":"ref14","first-page":"175","article-title":"Quantitative study of P substance in the megaesophagus and megacolon of human Trypanosoma cruzi infection","volume":"76","author":"Hial V","year":"1973","journal-title":"J Trop Med Hyg."}],"container-title":["Acta Cirurgica Brasileira"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/acb\/v26s2\/a14v26s2.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,13]],"date-time":"2025-03-13T08:35:07Z","timestamp":1741854907000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0102-86502011000800014&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011]]},"references-count":14,"journal-issue":{"issue":"suppl 2","published-print":{"date-parts":[[2011]]}},"alternative-id":["S0102-86502011000800014"],"URL":"https:\/\/doi.org\/10.1590\/s0102-86502011000800014","relation":{},"ISSN":["0102-8650"],"issn-type":[{"type":"electronic","value":"0102-8650"}],"subject":[],"published":{"date-parts":[[2011]]}}}