{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T21:29:24Z","timestamp":1768339764881,"version":"3.49.0"},"reference-count":10,"publisher":"FapUNIFESP (SciELO)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"published-print":{"date-parts":[[2002]]},"abstract":"<jats:p>The effect of 1%, 3% and 5% EGTA (ethylene glycol-bis-(b-amino-ethyl ether) N,N,N\u00b4,N\u00b4-tetra-acetic acid) on the microhardness of root dentin of the cervical third of human teeth was studied. Five newly extracted maxillary incisors were sectioned transversely at the cementoenamel junction, and the crowns were discarded. The roots were embedded in blocks of high-speed polymerized acrylic resin and cut transversely into 1-mm sections. The second section of the cervical third of the root of each tooth was sectioned and divided into four parts. Each part was placed on an acrylic disc that was used as a base for microhardness measurement. Fifty microliters of 1% EGTA, 3% EGTA, or 5% EGTA were applied to the dentin surface. Deionized and distilled water was used as control. Dentin microhardness was then measured with a load of 50 g for 15 s. Statistical analysis showed that the three concentrations of the chelating solution EGTA significantly reduced dentin microhardness when compared with water (ANOVA, p&lt;0.01), and that there was a statistically significant difference among the three solutions (Tukey test, p&lt;0.05).<\/jats:p>","DOI":"10.1590\/s0103-64402002000300009","type":"journal-article","created":{"date-parts":[[2005,4,18]],"date-time":"2005-04-18T11:03:56Z","timestamp":1113822236000},"page":"188-190","source":"Crossref","is-referenced-by-count":21,"title":["Effect of different EGTA concentrations on dentin microhardness"],"prefix":"10.1590","volume":"13","author":[{"given":"Antonio Miranda da","family":"Cruz-Filho","sequence":"first","affiliation":[{"name":"University of Ribeir\u00e3o Preto,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo Augusto de","family":"Paula","sequence":"additional","affiliation":[{"name":"University of Ribeir\u00e3o Preto,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesus Djalma","family":"P\u00e9cora","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manoel Dami\u00e3o de","family":"Sousa-Neto","sequence":"additional","affiliation":[{"name":"University of Ribeir\u00e3o Preto,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1097\/00004770-200205000-00004","article-title":"Effectiveness of manual and rotary instrumentation techniques for cleaning flattened root canals","volume":"28","author":"Barbizan JVB","year":"2002","journal-title":"J Endodon"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1097\/00004770-200203000-00010","article-title":"Effect of Er: YAG laser on adhesion of root canal sealer","volume":"28","author":"Sousa Neto MD","year":"2002","journal-title":"J Endodon"},{"key":"ref3","first-page":"3","article-title":"Chelation in root canal therapy","volume":"65","author":"\u00d8stby NB","year":"1957","journal-title":"Ethylenediamine tetra-acetic acid for leasing and widening of root canals. 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