{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T03:14:17Z","timestamp":1789528457175,"version":"build-2803163510"},"reference-count":19,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>It was assessed the immunohistochemical profile of CD25+ cells in cases of chronic gingivitis (CG) and chronic periodontitis (CP). Immunohistochemistry was carried out using streptoavidin-biotin complex and anti-CD25 antibody in 17 cases of CG and 25 cases of CP. Sixteen cases (94.1%) of CG were immunopositive. CD25 was focally expressed in 50% of the sample and diffusely expressed in 25%. The stained cells were localized not only beneath the epithelium, but also far from it. In relation to the cellular density quantification of CD25+ cells, score ++ was the most common. Concerning CP, all cases were immunopositive. CD25+ cells were expressed in focal or diffuse pattern either close or far from the epithelium. Diffuse distribution of positive cells throughout the connective tissue was seen in 60% of the cases and 32% showed focal or diffuse cellular pattern. Sixteen cases (64%) received score +++. It was identified that CD25+ cells are present in either a focal or a diffuse pattern in connective tissue. Significant differences in the density of cellular immunostaining between CG and CP were found. The greatest density was observed in CP cases, which suggests that the infiltrate of lymphocytes show a higher degree of cellular activation in periodontitis compared with gingivitis.<\/jats:p>","DOI":"10.1590\/s0103-64402012000400003","type":"journal-article","created":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T09:41:26Z","timestamp":1354354886000},"page":"322-327","source":"Crossref","is-referenced-by-count":6,"title":["Immunohistochemical evaluation of CD25+ cell expression in the progression of periodontal disease"],"prefix":"10.1590","volume":"23","author":[{"given":"Ruthin\u00e9ia Diog\u00e9nes Alves Uchoa","family":"Lins","sequence":"first","affiliation":[{"name":"State University of Para\u00edba,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pollianna Muniz","family":"Alves","sequence":"additional","affiliation":[{"name":"State University of Para\u00edba,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Pina","family":"Godoy","sequence":"additional","affiliation":[{"name":"State University of Para\u00edba,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ericka Janine Dantas","family":"Silveira","sequence":"additional","affiliation":[{"name":"Federal University of Rio Grande do Norte,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e9lia Maria Guedes","family":"Queiroz","sequence":"additional","affiliation":[{"name":"Federal University of Rio Grande do Norte,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roseana de Almeida","family":"Freitas","sequence":"additional","affiliation":[{"name":"Federal University of Rio Grande do Norte,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1038\/sj.bdj.2008.653","article-title":"Who is at risk? 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Rom J Morphol Embryol"},{"key":"ref8","first-page":"327","article-title":"A controversial role for IL-12 in immune response and bone resorption at apical periodontal sites","volume":"2010","author":"Queiroz-Junior CM","year":"2010","journal-title":"Clin Dev Immunol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1111\/j.1600-051X.2008.01298.x","article-title":"Cytokine gene polymorphisms in periodontal disease: a metaanalysis of 53 studies including 4178 cases and 4590 controls","volume":"35","author":"Nikolopoulos GK","year":"2008","journal-title":"J Clin Periodontol","ISSN":"https:\/\/id.crossref.org\/issn\/0303-6979","issn-type":"print"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1111\/j.1399-302X.2005.00245.x","article-title":"Cytokine pattern determines the progression of experimental periodontal disease induced by Actinobacillus actinomycetemcomitans through the modulation of MMPs, RANKL, and their physiological 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