{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T22:32:05Z","timestamp":1779143525563,"version":"3.51.4"},"reference-count":23,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>The aim of this study was to assess the immunohistochemical expression of p63 protein, epidermal growth factor receptor (EGFR) and Notch-1 in the epithelial lining of radicular cysts (RC), dentigerous cysts (DC) and keratocystic odontogenic tumors (KOT). For this study, 35 RC, 22 DC and 17 KOT were used. The clinical and epidemiological data were collected from the patient charts filed in the Oral Pathology Laboratory, University of Ribeir\u00e3o Preto, Brazil. Immunohistochemical reactions against the p63, EGFR and Notch-1 were performed in 3-\u00b5m-thick histological sections. The slides were evaluated according to the following criteria: negative: &lt;5% of positive cells, low expression: 5-50% of positive cells, and high expression: &gt;50% of positive cells. Moreover, the intensity of EGFR and Notch-1 expressions was also evaluated. Fisher's exact test and Spearman's correlation coefficients were used for statistical analysis, considering a significance level of 5%. Almost all cases demonstrated p63, EGFR and Notch-1 expressions. The p63 expression was significantly higher in KOT (p&lt;0.001). Positive correlation between these immunomarkers was observed. These findings suggest the participation of p63, EGFR and Notch-1 in the development, maintenance and integrity of cystic odontogenic epithelial lining, favoring lesion persistence. The high expression of p63 in KOT suggests that it may be related to their more aggressive biological behavior and marked tendency to recurrence.<\/jats:p>","DOI":"10.1590\/s0103-64402012000400005","type":"journal-article","created":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T09:41:32Z","timestamp":1354354892000},"page":"337-343","source":"Crossref","is-referenced-by-count":29,"title":["Immunohistochemical expression of p63, epidermal growth factor receptor (EGFR) and notch-1 in radicular cysts, dentigerous cysts and keratocystic odontogenic tumors"],"prefix":"10.1590","volume":"23","author":[{"given":"Cl\u00e1udia Kall\u00e1s","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"Federal University of Pernambuco,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo Rodrigues","family":"Fregnani","sequence":"additional","affiliation":[{"name":"Federal University of Pernambuco,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge Esquiche","family":"Leon","sequence":"additional","affiliation":[{"name":"Federal University of Pernambuco,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yara Teresinha Corr\u00eaa","family":"Silva-Sousa","sequence":"additional","affiliation":[{"name":"Federal University of Pernambuco,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danyel Elias da Cruz","family":"Perez","sequence":"additional","affiliation":[{"name":"Federal University of Pernambuco,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1034\/j.1601-0825.2003.02920.x","article-title":"Immunohistochemical demonstration of p63 in DMBA-induced hamster buccal pouch squamous cell carcinogenesis","volume":"9","author":"Chen YK","year":"2003","journal-title":"Oral Dis","ISSN":"https:\/\/id.crossref.org\/issn\/1354-523X","issn-type":"print"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1016\/j.ijom.2004.12.002","article-title":"p63 expression in odontogenic cysts","volume":"34","author":"Lo Muzio L","year":"2005","journal-title":"Int J Oral Maxillofac Surg","ISSN":"https:\/\/id.crossref.org\/issn\/0901-5027","issn-type":"print"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/S1368-8375(02)00034-9","article-title":"Epidermal growth factor receptor expression in ameloblastoma","volume":"39","author":"Vered M","year":"2003","journal-title":"Oral Oncol","ISSN":"https:\/\/id.crossref.org\/issn\/1368-8375","issn-type":"print"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1136\/mp.50.1.21","article-title":"Immunocytochemical expression of growth factors by odontogenic jaw cysts","volume":"50","author":"Li TJ","year":"1997","journal-title":"Mol Pathol","ISSN":"https:\/\/id.crossref.org\/issn\/1366-8714","issn-type":"print"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/S0003-9969(99)00070-9","article-title":"The immunohistochemical localization of phospholipase c\u03b3 and the epidermal growth-factor, platelet-derived growth-factor and fibroblast growth-factor receptors in the cells of the rat molar enamel organ during early amelogenesis","volume":"44","author":"Tanikawa Y","year":"1999","journal-title":"Arch Oral Biol","ISSN":"https:\/\/id.crossref.org\/issn\/0003-9969","issn-type":"print"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.joen.2010.10.007","article-title":"Immunohistochemical expression of notch signaling in the lining epithelium of periapical cysts","volume":"37","author":"Meliou E","year":"2011","journal-title":"J 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the developing mouse tooth and associated with determination of ameloblast cell fate","volume":"130","author":"Mitsiadis TA","year":"1995","journal-title":"J Cell Biol","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9525","issn-type":"print"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"3427","DOI":"10.1093\/emboj\/20.13.3427","article-title":"Notch signaling is a direct determinant of keratinocyte growth arrest and entry into differentiation","volume":"20","author":"Miele L","year":"2001","journal-title":"EMBO J","ISSN":"https:\/\/id.crossref.org\/issn\/0261-4189","issn-type":"print"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1046\/j.1399-0004.2003.00194.x","article-title":"Notch signaling in development and disease","volume":"64","author":"Harper JA","year":"2003","journal-title":"Clin 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