{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T18:05:41Z","timestamp":1775239541937,"version":"3.50.1"},"reference-count":44,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"published-print":{"date-parts":[[2004,5]]},"abstract":"<jats:p>Final oocyte maturation (FOM) is a process involving a complex set of genetical, biochemical, and morphological mechanisms. FOM involves the shift of a post-vitellogenic follicle to a pre-ovulated oocyte, which is necessary for fertilization by spermatozoan to occur. This process is regulated by a maturation-inducing steroid (MIS) at the follicular level. In other species of scienids fish the MIS, a hydroxilated derivatives of progestagen 17, 20beta, 21-trihydroxy-4-pregnen-3-one (20beta-S), was identified. Although Micropogonias furnieri is the second fishery resource of Uruguay, basic knowledge about its endocrine process is very scarce. The aim of this work was to investigate what steroids are synthesized in vitro by the oocyte follicle of M. furnieri during the maturation process. Fragments of ovary (1 g) in three stages: post-vitellogenic (PV), maturing (Mtg), and mature (M) were incubated with 1 mug.g-1 of tritiated progesterone (P) at 30, 60, and 180 min. After extraction with ethanol and dichloromethane, steroid metabolites were purified by TLC and rpHPLC. Two progesterone derivatives with identical chromatographic properties of 20beta-S and 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P) were purified. In other Teleost fish these steroids are biologically activ as MIS. The 17,20beta-P was clearly detected in Mtg and M stages and confirmed by enzymatic oxidation with enzyme 20beta-HSD. The 20beta-S was strongly detected in all Mtg oocytes. The results do not corroborate 20beta-S as a major hormone synthesized in the ovary in FOM as occurs in other scienid fish. A differential steroid synthesis in the advanced oocyte stages suggests that the 20beta-S is acting as a MIS in M. furnieri.<\/jats:p>","DOI":"10.1590\/s1519-69842004000200005","type":"journal-article","created":{"date-parts":[[2005,4,18]],"date-time":"2005-04-18T07:06:54Z","timestamp":1113808014000},"page":"211-220","source":"Crossref","is-referenced-by-count":8,"title":["Steroid metabolism in vitro during final oocyte maturation in white croaker Micropogonias furnieri (Pisces: Scianidae)"],"prefix":"10.1590","volume":"64","author":[{"given":"J.","family":"Garc\u00eda-Alonso","sequence":"first","affiliation":[{"name":"Facultad de Ciencias,  Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Nappa","sequence":"additional","affiliation":[{"name":"Facultad de Qu\u00edmica,  Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Somoza","sequence":"additional","affiliation":[{"name":"IIB-INTECH,  Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Rey","sequence":"additional","affiliation":[{"name":"Facultad de Qu\u00edmica,  Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Vizziano","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias,  Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","first-page":"7","article-title":"Estuarine spawning of the whitemouth croaker Micropogonias furnieri (Pisces: Sciaenidae), in the R\u00edo de la Plata, Argentina","volume":"50","author":"ACHA E. M.","year":"1999","journal-title":"Mar. Fresh. Res."},{"key":"ref2","series-title":"Biosynthese du steroide inducteur de la maturation ovocytaire par les cellules de granulosa du follicle ovarien de truite arc-en-ciel, Oncorhynchus mykiss","first-page":"71","author":"BAEK H.","year":"1990"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/0016-6480(88)90262-6","article-title":"Structure-activity relationships of C21 steroids in an \"in vitro\" oocyte maturation bioassay in rainbow trout, Salmo gairdneri","volume":"71","author":"CANARIO A. V. M.","year":"1988","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref4","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/0016-6480(89)90116-0","article-title":"Radioimmunoassay investigations of 20 -hydroxilated steroids in maturing\/ovulating female rainbow trout (Salmo gairdneri)","volume":"74","author":"CANARIO A. V. M.","year":"1989","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref5","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/0016-6480(90)90014-D","article-title":"Identification of, and development of radioimmunoassays for 17, 21-dihydroxy-4-pregnen-3, 20-dione and 3, 17, 21-trihydroxy-4-pregnen-3, 20dione in the ovaries of mature plaice (Pleuronectes platessa)","volume":"78","author":"CANARIO A. V. M.","year":"1990","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref6","series-title":"Fish Physiology vol. IXA","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/S1546-5098(08)60307-6","volume-title":"The gonadal steroids","volume":"9","author":"FOSTIER A.","year":"1983"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/BF02180281","article-title":"Hormonal changes during meiotic maturation and ovulation in the brook trout (Salvelinus fontinalis)","volume":"3","author":"GOETZ F. W.","year":"1987","journal-title":"Fish Physiol. Biochem."},{"key":"ref8","series-title":"Approches morphologique, histologique et endocrinienne des cycles reproducteurs et de l'inversion sexuelle chez un poisson hermafrodite protandre, le loup tropical Lates calcarifer, introduit en elevage en Polynesie Francaise","first-page":"101","author":"GUIGUEN Y.","year":"1992"},{"key":"ref9","series-title":"Synopsis of biological data on the whitemouth croaker Micropogonias furnieri (Desmarest, 1823): FAO Fisheries Synopsis","first-page":"35","volume":"150","author":"ISAAC V. J.","year":"1988"},{"key":"ref10","first-page":"1205","article-title":"Action de la 17 -hydroxy, 20 -dihydroprogesterone et de la progest\u00e9rone sur la maturation et l'ovulation in vivo et sur le niceau d'hormone gonadotrope plasmatique t-GtH chez la truite arc-en-ciel (Salmo gairdneri) C.R. Hebd. Seances.","volume":"283","author":"JALABERT B.","year":"1976","journal-title":"Acad. Sci. Ser. D"},{"key":"ref11","series-title":"Vertebrates endocrinology: fundamentals and biomedical implications in reproduction","first-page":"23","volume-title":"Oocyte maturation in vertebrates","volume":"4","author":"JALABERT B.","year":"1991"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1006\/gcen.1998.7133","article-title":"GTH II but not GTH I induces final maturation and the development of maturational competence of oocytes of red Seabream in vitro","volume":"112","author":"KAGAWA H.","year":"1998","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref13","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1007\/BF02274230","article-title":"Progesteron metabolism by ovaries of the roach (Rutilus rutilus L.) and the rudd (Scardinius erytrophthalmus)","volume":"9","author":"KIME D. E.","year":"1992","journal-title":"Fish Physiol. Biochem."},{"key":"ref14","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1007\/BF00045230","article-title":"\"Classical\" and \"Non-classical\" reproductive steroids in fish","volume":"3","author":"KIME D. E.","year":"1993","journal-title":"Rev. Fish. Biol."},{"key":"ref15","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1006\/gcen.1994.1177","article-title":"Hormonal regulation of final Maturation of Striped Bass oocyte in vitro","volume":"96","author":"KING W. V.","year":"1994","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref16","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1111\/j.1095-8649.1992.tb02643.x","article-title":"Melano-macrophage centres in whitemouth croaker, (Micropogonias furnieri), as a biological indicator of environmental changes","volume":"40","author":"MACCHI G.","year":"1992","journal-title":"J. Fish Biol.","ISSN":"https:\/\/id.crossref.org\/issn\/0022-1112","issn-type":"print"},{"key":"ref17","author":"MOSES R.","year":"0000"},{"key":"ref18","series-title":"Analyse morphologique et endocrinienne des derni\u00e8res \u00e9tapes de l\u00b4ovog\u00e9n\u00e8se et recherche d\u00b4un traitement de l\u00b4induction de l\u00b4ovulation chez le turbot Scophtalmus maximus L.","first-page":"100p","author":"MUGNIER C.","year":"1996"},{"key":"ref19","series-title":"Fish Physiology: Endocrine tissues and hormones","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S1546-5098(08)60290-3","volume-title":"The functional morphology of teleost gonads","volume":"9","author":"NAGAHAMA Y","year":"1983"},{"key":"ref20","first-page":"9","author":"NAGAHAMA Y.","year":"1983"},{"key":"ref21","first-page":"217","article-title":"Endocrine control of gametogenesis","volume":"38","author":"NAGAHAMA Y.","year":"1994","journal-title":"Int. J. Dev. Biol."},{"key":"ref22","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1002\/jez.1402550113","article-title":"Induction of maturation of atlantic croaker oocyte by 17, 20, 21-Trihydroxy-4-pregnen-3-one in vitro: consideration of some biological and experimental variables","volume":"255","author":"PATI\u00d1O R.","year":"1990","journal-title":"J. Exp. Zool."},{"key":"ref23","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1095\/biolreprod43.5.818","article-title":"Effects of gonadotropin on ovarian intrafollicular processes during the development of oocyte maturation competence in a Teleost, the Atlantic croaker: evidence for two distinct stages of gonadotropic control of final oocyte maturation","volume":"43","author":"PATI\u00d1O R.","year":"1990","journal-title":"Biol. Reprod."},{"key":"ref24","author":"PATI\u00d1O R.","year":"2000"},{"key":"ref25","series-title":"Estructura poblacional, mortalidad y uso de modelos de producci\u00f3n captura-mortalidad en la pesquer\u00eda de corvina Micropogonias furnieri (Desmarest, 1823) de la zona com\u00fan de pesca argentino-uruguaya","author":"PIN D.","year":"1999"},{"key":"ref26","series-title":"The physiology of fishes","first-page":"592","volume-title":"Reproductive Physiology","author":"REDDING J. M.","year":"1993"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0305-0491(98)10130-X","article-title":"Steroid metabolism by ovarian follicles of the tilapia Oreochromis mossambicus (Teleostei, Cichlidae)","volume":"121","author":"ROCHA M. J.","year":"1998","journal-title":"Comp. Biochem. Physiol."},{"key":"ref28","series-title":"Proceedings of the 3rd International Symposium on the Reproductive Physiology of Fish","first-page":"224","volume-title":"Status of oocyte maturation-inducing steroids in Teleost","author":"SCOTT A. P.","year":"1987"},{"key":"ref29","author":"SCOTT A. P.","year":"1991"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/j.1095-8649.1994.tb01590.x","article-title":"Urine of reproductively mature female rainbow trout, Oncorhynchus mykiss (Walbaum), contains a priming pheromone which enhances plasma levels of sex steroid and gonadotrophin II in males","volume":"44","author":"SCOTT A. P.","year":"1994","journal-title":"J. Fish Biol.","ISSN":"https:\/\/id.crossref.org\/issn\/0022-1112","issn-type":"print"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/0016-6480(81)90051-4","article-title":"In vitro metabolism of 4-pregnenes in ovaries of a freshwater Teleost, the ayu (Plecoglossus altivelis): Production of 17, 20-dihydroxy-4-pregnen-3-one and its 5 -reduced metabolites","volume":"45","author":"SUZUKI K.","year":"1981","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref32","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/BF00004706","article-title":"20, 21-trihydroxy-4-pregnen-3-one is a maturation-inducing steroid in spotted seatrout","volume":"7","author":"THOMAS P. & TRANT","year":"1981","journal-title":"Fish Physiol. Biochem."},{"key":"ref33","series-title":"Perspectives in Comparative Endocrinology","first-page":"619","volume-title":"Hormonal control of final oocyte maturation in sciaenid fishes","author":"THOMAS P.","year":"1994"},{"key":"ref34","series-title":"Proceeding of Third International Symposium of Reproductive Physiology of Fish","first-page":"219","volume-title":"Plasma levels of gonadal steroids during the reproductive cycle of female spotted seatrout, Cynoscion nebulosus","author":"THOMAS P.","year":"1987"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/0039-128X(86)90081-4","article-title":"Identification of 17, 20, 21-Trihydroxy-4-pregnen-3-one as the major ovarian steroid produced by the Teleost (Micropogonias undulatus) during final oocyte maturation","volume":"47","author":"TRANT J. M.","year":"1986","journal-title":"Steroids","ISSN":"https:\/\/id.crossref.org\/issn\/0039-128X","issn-type":"print"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/0016-6480(88)90259-6","article-title":"Structure-activity relationships of steroids in inducing germinal vesicle breakdown of Atlantic croaker oocyte in vitro","volume":"71","author":"TRANT J. M.","year":"1988","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref37","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/0016-6480(89)90174-3","article-title":"Isolation of a novel maturation-inducing steroid produced in vitro by ovaries of Atlantic croaker","volume":"75","author":"TRANT J. M.","year":"1989","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref38","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/0016-6480(89)90175-5","article-title":"Changes in ovarian steroidogenesis in vitro associated with final maturation of Atlantic croakers oocytes","volume":"75","author":"TRANT J. M.","year":"1986","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref39","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/0016-6480(86)90183-8","article-title":"Germinal vesicle breakdown in oocytes of catfish, Mystus vitatus (Bloch): Relative in vitro effectiveness of estradiol-17, androgens, corticosteroids, progesterone, and other pregnene derivatives","volume":"63","author":"UPADHYAYA N.","year":"1986","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref40","first-page":"27","article-title":"Micropogon furnieri: Fecundidade e tipo de desova","volume":"18(1)","author":"VAZZOLER A. E. A.","year":"1970","journal-title":"Bolm. Inst. Oceanog. S Paulo"},{"key":"ref41","series-title":"Biologia da reprodu\u00e7\u00e3o de peixes Tele\u00f3steos: Teoria e Pr\u00e1ctica","first-page":"169","author":"VAZZOLER A. E. A.","year":"1998"},{"key":"ref42","author":"VIZZIANO D.","year":"0000"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1006\/gcen.1996.0105","article-title":"Milt and endocrine responses of mature male Atlantic salmon (Salmo salar L.) parr to water-borne testosterone, 17, 20 -dihydroxy-4-pregnen-3-one 20-sulfate, and the urines from adult female and male salmon","volume":"103","author":"WARING C. P.","year":"1996","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref44","first-page":"74","author":"YUELH W. H.","year":"2000"}],"container-title":["Brazilian Journal of Biology"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/bjb\/v64n2\/v64n2a05.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,5,24]],"date-time":"2017-05-24T23:43:09Z","timestamp":1495669389000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842004000200005&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,5]]},"references-count":44,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,5]]}},"alternative-id":["S1519-69842004000200005"],"URL":"https:\/\/doi.org\/10.1590\/s1519-69842004000200005","relation":{},"ISSN":["1519-6984"],"issn-type":[{"value":"1519-6984","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,5]]}}}