{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:26:26Z","timestamp":1790735186538,"version":"4.1.0"},"reference-count":38,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Einstein (S\u00e3o Paulo)"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:p>OBJECTIVE: To establish a routine for the extraction of the total levels of CoQ10 in human plasma through the Ultra High Performance Liquid Chromatography (UHPLC). METHODS: Two extraction protocols were tested: a) methanol: hexane and b) 1-propanol. The following parameters were analyzed: extraction temperature (19\u00baC and 4\u00baC), extraction tubes (glass and polypropylene), and surfactants (SDS, Triton X-100, Tween-20) at different concentrations, i.e., 1%, 3%, 5% and 10%. RESULTS: The results showed that the method of extraction of CoQ10 in a sample of human plasma at 4\u00baC, using solvents methanol: hexane (85:15, v\/v) in the presence of surfactant Tween-20 at 3% and polypropylene tubes showed better efficiency and reproducibility when compared to the method with 1-propanol. CONCLUSION: By the analyses performed, it was possible to observe that the addition of the surfactant Tween-20 promoted an increase in the recovery of CoQ10 by the methanol:hexane extraction method. This method showed good reproducibility, with a low coefficient of variation and high sensitivity, since CoQ10 was detected in samples of plasma of a control individual using a UV-type detector. The use of UHPLC equipment allowed a total analysis with total run time of 3.5 minutes, enabling the rapid achievement of results, considered mandatory for laboratory routines.<\/jats:p>","DOI":"10.1590\/s1679-45082012000200014","type":"journal-article","created":{"date-parts":[[2012,9,22]],"date-time":"2012-09-22T08:40:30Z","timestamp":1348303230000},"page":"203-208","source":"Crossref","is-referenced-by-count":5,"title":["Determination of a method for extraction of coenzyme Q10 in human plasma: optimization of the use of surfactants and other variables"],"prefix":"10.1590","volume":"10","author":[{"given":"Claudia Cristina","family":"Ferreiro-Barros","sequence":"first","affiliation":[{"name":"Instituto do C\u00e9rebro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo Kinio","family":"Sugawara","sequence":"additional","affiliation":[{"name":"Hospital Israelita Albert Einstein, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Livia Rentas","family":"Sanches","sequence":"additional","affiliation":[{"name":"Hospital Israelita Albert Einstein, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.nmd.2011.05.001","article-title":"176th ENMC International Workshop: Diagnosis and treatment of coenzyme Q10 deficiency","volume":"22","author":"Rahman S","year":"2012","journal-title":"Neuromus Disord"},{"issue":"1-2","key":"ref2","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.bbamem.2003.11.012","article-title":"Metabolism and function of coenzyme Q","volume":"1660","author":"Turunen M","year":"2004","journal-title":"Biochim Biophys Acta","ISSN":"https:\/\/id.crossref.org\/issn\/0167-4889","issn-type":"print"},{"issue":"4-5","key":"ref3","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1007\/s11064-006-9190-z","article-title":"Human coenzyme Q10 deficiency","volume":"32","author":"Quinzii CM","year":"2007","journal-title":"Neurochem Res","ISSN":"https:\/\/id.crossref.org\/issn\/0364-3190","issn-type":"print"},{"issue":"7","key":"ref4","doi-asserted-by":"crossref","first-page":"2379","DOI":"10.1073\/pnas.86.7.2379","article-title":"Muscle coenzyme Q deficiency in familial mitochondrial encephalomyopathy","volume":"86","author":"Ogasahara S","year":"1989","journal-title":"Proc Natl Acad Sci U S A","ISSN":"https:\/\/id.crossref.org\/issn\/0027-8424","issn-type":"print"},{"issue":"7","key":"ref5","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1212\/WNL.56.7.849","article-title":"Familial cerebellar ataxia with muscle coenzyme Q10 deficiency","volume":"56","author":"Musumeci O","year":"2001","journal-title":"Neurology","ISSN":"https:\/\/id.crossref.org\/issn\/0028-3878","issn-type":"print"},{"issue":"2","key":"ref6","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1080\/14734220601021700","article-title":"Clinical, biochemical and molecular aspects of cerebellar ataxia and Coenzyme Q10 deficiency","volume":"6","author":"Montero R","year":"2007","journal-title":"Cerebellum"},{"issue":"4","key":"ref7","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1212\/01.wnl.0000172859.55579.a7","article-title":"Infantile encephalomyopathy and nephropathy with CoQ10 deficiency: a CoQ10-responsive condition","volume":"65","author":"Salviati L","year":"2005","journal-title":"Neurology","ISSN":"https:\/\/id.crossref.org\/issn\/0028-3878","issn-type":"print"},{"issue":"5","key":"ref8","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1212\/01.WNL.0000113719.67643.B7","article-title":"Late-onset cerebellar ataxia with hypogonadism and muscle coenzyme Q10 deficiency","volume":"62","author":"Gironi M","year":"2004","journal-title":"Neurology","ISSN":"https:\/\/id.crossref.org\/issn\/0028-3878","issn-type":"print"},{"issue":"3","key":"ref9","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.ajhg.2007.12.024","article-title":"ADCK3, an ancestral kinase, is mutated in a form of recessive ataxia associated with coenzyme Q10 deficiency","volume":"82","author":"Lagier-Tourenne C","year":"2008","journal-title":"Am J Hum Genet","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9297","issn-type":"print"},{"issue":"4","key":"ref10","doi-asserted-by":"crossref","first-page":"2676","DOI":"10.1074\/jbc.M411527200","article-title":"Genetic evidence for a multi-subunit complex in coenzyme Q biosynthesis in yeast and the role of the Coq1 hexaprenyl diphosphate synthase","volume":"280","author":"Gin P","year":"2005","journal-title":"J Biol Chem","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"issue":"3","key":"ref11","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1128\/MMBR.54.3.211-225.1990","article-title":"PET genes of Saccharomyces cerevisiae","volume":"54","author":"Tzagoloff A","year":"1990","journal-title":"Microbiol Rev","ISSN":"https:\/\/id.crossref.org\/issn\/0146-0749","issn-type":"print"},{"issue":"36","key":"ref12","doi-asserted-by":"crossref","first-page":"31397","DOI":"10.1074\/jbc.M503277200","article-title":"COQ9, a new gene required for the biosynthesis of coenzyme Q in Saccharomyces cerevisiae","volume":"280","author":"Johnson A","year":"2005","journal-title":"J Biol Chem","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"issue":"52","key":"ref13","doi-asserted-by":"crossref","first-page":"42627","DOI":"10.1074\/jbc.M510768200","article-title":"The Saccharomyces cerevisiae COQ10 gene encodes a START domain protein required for function of coenzyme Q in respiration","volume":"280","author":"Barros MH","year":"2005","journal-title":"Biol Chem","ISSN":"https:\/\/id.crossref.org\/issn\/1431-6730","issn-type":"print"},{"issue":"2","key":"ref14","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1086\/500092","article-title":"A mutation in para-hydroxybenzoate-polyprenyl transferase (COQ2) causes primary coenzyme Q10 deficiency","volume":"78","author":"Quinzii C","year":"2006","journal-title":"Am J Hum Genet","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9297","issn-type":"print"},{"issue":"3","key":"ref15","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1136\/jmedgenet-2011-100394","article-title":"Haploinsufficiency of COQ4 causes coenzyme Q10 deficiency","volume":"49","author":"Salviati L","year":"2012","journal-title":"J Med Genet","ISSN":"https:\/\/id.crossref.org\/issn\/0022-2593","issn-type":"print"},{"issue":"5","key":"ref16","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1172\/JCI45693","article-title":"COQ6 mutations in human patients produce nephrotic syndrome with sensorineural deafness","volume":"121","author":"Heeringa SF","year":"2011","journal-title":"J Clin Invest","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9738","issn-type":"print"},{"issue":"3","key":"ref17","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/j.ajhg.2007.12.022","article-title":"CABC1 gene mutations cause ubiquinone deficiency with cerebellar ataxia and seizures","volume":"82","author":"Mollet J","year":"2008","journal-title":"Am J Hum Genet","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9297","issn-type":"print"},{"issue":"5","key":"ref18","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1016\/j.ajhg.2009.03.018","article-title":"A nonsense mutation in COQ9 causes autosomal-recessive neonatal-onset primary coenzyme Q10 deficiency: a potentially treatable form of mitochondrial disease","volume":"84","author":"Duncan AJ","year":"2009","journal-title":"Am J Hum Genet","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9297","issn-type":"print"},{"issue":"3","key":"ref19","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1172\/JCI29089","article-title":"Prenyldiphosphate synthase, subunit 1 (PDSS1) and OH-benzoate polyprenyltransferase (COQ2) mutations in ubiquinone deficiency and oxidative phosphorylation disorders","volume":"117","author":"Mollet J","year":"2007","journal-title":"J Clin Invest","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9738","issn-type":"print"},{"issue":"6","key":"ref20","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1086\/510023","article-title":"Leigh syndrome with nephropathy and CoQ10 deficiency due to decaprenyl diphosphate synthase subunit 2 (PDSS2) mutations","volume":"79","author":"L\u00f3pez LC","year":"2006","journal-title":"Am J Hum Genet","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9297","issn-type":"print"},{"issue":"11","key":"ref21","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1001\/archneur.62.11.1709","article-title":"Muscle coenzyme Q10 level in statin-related myopathy","volume":"62","author":"Lamperti C","year":"2005","journal-title":"Arch Neurol","ISSN":"https:\/\/id.crossref.org\/issn\/0003-9942","issn-type":"print"},{"issue":"2","key":"ref22","first-page":"71","article-title":"Coenzyme q10: is there a clinical role and a case for measurement?","volume":"29","author":"Molyneux SL","year":"2008","journal-title":"Clin Biochem Rev","ISSN":"https:\/\/id.crossref.org\/issn\/0159-8090","issn-type":"print"},{"issue":"1","key":"ref23","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0925-4439(95)00028-3","article-title":"Biochemical, physiological and medical aspects of ubiquinone function","volume":"1271","author":"Ernster L","year":"1995","journal-title":"Biochim Biophys Acta","ISSN":"https:\/\/id.crossref.org\/issn\/0167-4889","issn-type":"print"},{"issue":"12","key":"ref24","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1016\/j.bbadis.2008.10.015","article-title":"A critical approach to the therapy of mitochondrial respiratory chain and oxidative phosphorylation diseases","volume":"1792","author":"Dimauro S","year":"2009","journal-title":"Biochim Biophys Acta","ISSN":"https:\/\/id.crossref.org\/issn\/0167-4889","issn-type":"print"},{"issue":"6","key":"ref25","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1080\/00365519950185481","article-title":"Measurement and stability of plasma reduced, oxidized and total coenzyme Q10 in humans","volume":"59","author":"Kaikkonen J","year":"1999","journal-title":"Scand J Clin Lab Invest","ISSN":"https:\/\/id.crossref.org\/issn\/0036-5513","issn-type":"print"},{"issue":"1","key":"ref26","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/0378-4347(88)80009-4","article-title":"Determination of coenzyme Q10, alpha-tocopherol and cholesterol in biological samples by coupled-column liquid chromatography with coulometric and ultraviolet detection","volume":"425","author":"Edlund PO","year":"1988","journal-title":"J Chromatogr","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9673","issn-type":"print"},{"issue":"2","key":"ref27","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1093\/clinchem\/47.2.256","article-title":"HPLC analysis of reduced and oxidized coenzyme Q(10) in human plasma","volume":"47","author":"Tang PH","year":"2001","journal-title":"Clin Chem","ISSN":"https:\/\/id.crossref.org\/issn\/0009-9147","issn-type":"print"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/S0076-6879(04)78014-3","article-title":"Assay of coenzyme Q10 in plasma by a single dilution step","volume":"378","author":"Littarru GP","year":"2004","journal-title":"Methods Enzymol","ISSN":"https:\/\/id.crossref.org\/issn\/0076-6879","issn-type":"print"},{"issue":"1","key":"ref29","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0378-4347(00)83129-1","article-title":"Determination of reduced and total ubiquinones in biological materials by liquid chromatography with electrochemical detection","volume":"430","author":"Okamoto T","year":"1988","journal-title":"J Chromatogr","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9673","issn-type":"print"},{"issue":"6","key":"ref30","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1016\/S0731-7085(97)00057-5","article-title":"A simplified chromatographic method for quantitative determination of coenzyme Q10 in dog plasma","volume":"16","author":"Kommuru TR","year":"1998","journal-title":"J Pharm Biomed Anal","ISSN":"https:\/\/id.crossref.org\/issn\/0731-7085","issn-type":"print"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0076-6879(84)05020-5","article-title":"Simultaneous determination of reduced and oxidezed coenzyme Q10 in human plasma","volume":"105","author":"Takada M","year":"1984","journal-title":"Methods Enzymol","ISSN":"https:\/\/id.crossref.org\/issn\/0076-6879","issn-type":"print"},{"issue":"1","key":"ref32","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1006\/abio.1997.2187","article-title":"Simultaneous detection of ubiquinol and ubiquinone in human plasma as a marker of oxidative stress","volume":"250","author":"Yamashita S","year":"1997","journal-title":"Anal Biochem","ISSN":"https:\/\/id.crossref.org\/issn\/0003-2697","issn-type":"print"},{"issue":"1-2","key":"ref33","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/S0378-4347(99)00067-5","article-title":"Automated high-performance liquid chromatographic method with precolumn reduction for the determination of ubiquinol and ubiquinone in human plasma","volume":"726","author":"Wang Q","year":"1999","journal-title":"J Chromatogr B Biomed Sci Appl."},{"issue":"2","key":"ref34","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.jpba.2006.03.037","article-title":"HPLC method for simultaneous determination of retinol, alpha-tocopherol and coenzyme Q10 in human plasma","volume":"42","author":"Karpi\u0144ska J","year":"2006","journal-title":"J Pharm Biomed Anal."},{"key":"ref35","doi-asserted-by":"crossref","first-page":"S105","DOI":"10.1016\/S0098-2997(97)00039-3","article-title":"Oxidation of LDL and their subfractions: kinetic aspects and CoQ10 content","volume":"18","author":"Alleva R","year":"1997","journal-title":"Mol Aspects Med."},{"issue":"1-2","key":"ref36","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.cccn.2004.06.015","article-title":"Comparison of coenzyme Q10 plasma levels in obese and normal weight children","volume":"349","author":"Menke T","year":"2004","journal-title":"Clin Chim Acta","ISSN":"https:\/\/id.crossref.org\/issn\/0009-8981","issn-type":"print"},{"issue":"1","key":"ref37","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/0378-4347(84)80083-3","article-title":"Effect of sodium dodecyl sulphate on the extraction of ubiquinone-10 in the determination of plasma samples","volume":"310","author":"Hirota K","year":"1984","journal-title":"J Chromatogr","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9673","issn-type":"print"},{"issue":"3","key":"ref38","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1111\/j.1432-1033.1990.tb15449.x","article-title":"The interaction of Triton X-100 with purple membranes: Detergent binding, spectral changes and membrane solubilization","volume":"188","author":"Gonz\u00e1lez-Ma\u00f1as JM","year":"1990","journal-title":"Eur J Biochem","ISSN":"https:\/\/id.crossref.org\/issn\/0014-2956","issn-type":"print"}],"container-title":["Einstein (S\u00e3o Paulo)"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/eins\/v10n2\/a14v10n2.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T03:13:36Z","timestamp":1594696416000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1679-45082012000200014&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6]]},"references-count":38,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,6]]}},"alternative-id":["S1679-45082012000200014"],"URL":"https:\/\/doi.org\/10.1590\/s1679-45082012000200014","relation":{},"ISSN":["1679-4508"],"issn-type":[{"value":"1679-4508","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6]]}}}