{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T14:55:15Z","timestamp":1649861715972},"reference-count":27,"publisher":"FapUNIFESP (SciELO)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. bras. pneumol."],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:p>OBJETIVO: Identificar as esp\u00e9cies de micobact\u00e9rias encontradas no escarro de pacientes com suspeita de tuberculose pulmonar e analisar o impacto dessas identifica\u00e7\u00f5es na abordagem terap\u00eautica. M\u00c9TODOS: Foram avaliados 106 pacientes com suspeita de tuberculose pulmonar encaminhados para o servi\u00e7o de pneumologia de um hospital p\u00fablico em Teresina, Piau\u00ed. Esp\u00e9cimes de escarro matinal foram avaliados quanto \u00e0 presen\u00e7a de micobact\u00e9rias por baciloscopia e cultura. Foram utilizadas PCR e an\u00e1lise de restri\u00e7\u00e3o enzim\u00e1tica do gene hsp65 (PRA-hsp65) para a identifica\u00e7\u00e3o das cepas de micobact\u00e9rias isoladas em cultura. RESULTADOS: Foram analisadas 206 amostras de escarro. A idade dos pacientes variou de 15 a 87 anos, sendo 67% do g\u00eanero masculino. Tosse ocorreu em 100% dos casos. O padr\u00e3o radiogr\u00e1fico predominante foi de les\u00e3o moderada, observada em 70%. A positividade no esfrega\u00e7o foi de 76%, e isolamento em cultura ocorreu em 91% das culturas executadas. Testes tradicionais identificaram micobact\u00e9rias n\u00e3o tuberculosas (MNT) em 9% dos isolados. O m\u00e9todo PRA-hsp65 confirmou esses dados, mostrando sete padr\u00f5es de bandas capazes de identificar as esp\u00e9cies de MNT isoladas: Mycobacterium kansasii; M. abscessus 1; M. abscessus 2; M. smegmatis; M. flavescens 1; M. gordonae 5 e M. gordonae 7. Todos os pacientes com MNT tinham mais de 60 anos, e observaram-se bronquiectasias em 88% das radiografias. Houve dois casos de reinfec\u00e7\u00e3o, identificados inicialmente como infec\u00e7\u00e3o por M. abscessus e M. kansasii. CONCLUS\u00d5ES: As MNT causam infec\u00e7\u00e3o pulmonar em pacientes imunocompetentes, e a identifica\u00e7\u00e3o das MNT \u00e9 importante para estabelecer o diagn\u00f3stico correto e a decis\u00e3o terap\u00eautica mais adequada. O m\u00e9todo PRA-hsp65 \u00e9 \u00fatil para identificar esp\u00e9cies de MNT e pode ser implantado em laborat\u00f3rios de biologia molecular n\u00e3o especializados em micobact\u00e9rias.<\/jats:p>","DOI":"10.1590\/s1806-37132011000500010","type":"journal-article","created":{"date-parts":[[2017,3,29]],"date-time":"2017-03-29T19:22:44Z","timestamp":1490815364000},"page":"628-635","source":"Crossref","is-referenced-by-count":2,"title":["An\u00e1lise de restri\u00e7\u00e3o enzim\u00e1tica do gene hsp65 de isolados cl\u00ednicos de pacientes com suspeita de tuberculose pulmonar em Teresina, Piau\u00ed"],"prefix":"10.1590","volume":"37","author":[{"given":"Maria das Gra\u00e7as Motta e","family":"Bona","sequence":"first","affiliation":[{"name":"Universidade Federal do Piau\u00ed, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Jos\u00e9 Soares","family":"Leal","sequence":"additional","affiliation":[{"name":"Universidade Federal do Piau\u00ed, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liline Maria Soares","family":"Martins","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Piau\u00ed, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raimundo Nonato da","family":"Silva","sequence":"additional","affiliation":[{"name":"Universidade Federal do Piau\u00ed, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Adail Fonseca de","family":"Castro","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Piau\u00ed, Brasil; Universidade Federal do Piau\u00ed, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Semiramis Jamil Hadad do","family":"Monte","sequence":"additional","affiliation":[{"name":"Universidade Federal do Piau\u00ed, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","series-title":"Global Tuberculosis Control: Epidemiology, 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