{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:06:51Z","timestamp":1760645211086},"reference-count":27,"publisher":"FapUNIFESP (SciELO)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. oral res."],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:p>A histological study was conducted of the alveolar bone healing process following tooth extraction of dehydrated rats after the implantation of fibrin adhesive (TISSUCOL\u2122) associated to previous irrigation of the wound with a 5% epsilon aminocaproic acid solution (EACA). Seventy two rats were used, divided into three groups receiving different treatments after the surgical procedure. In group I, the gingival mucosa was sutured after extraction of the right upper incisor. In groups II and III, chronic dehydration was produced by water deprivation for 9 days (3 days in the preoperative period and 6 days in the postoperative period). In the animals of Group II, after tooth extraction, the gingival mucosa was sutured in the same way as performed in group I. In group III, after extraction, the dental socket was irrigated with 5% EACA, followed by implantation of the fibrin adhesive (TISSUCOL\u2122). The mucosa was sutured in the same way as performed in the other groups. At 3, 7, 15 and 21 postoperative days, the animals were sacrificed in number of 6 for each group. Specimens containing the dental socket were removed and fixed in 10% formalin and decalcified in an equal part formic acid and sodium citrate solution. After routine processing, the specimens were embedded in paraffin for microtomy. We obtained 6 \u00b5m semi-serial slices that were stained with hematoxylin and eosin for histological evaluation. The results showed that the water deprivation in the pre- and postoperative periods caused a delay in the alveolar bone healing process. The use of the fibrin adhesive (TISSUCOL\u2122) produced an improvement in the fibrinolytic picture caused by dehydration.<\/jats:p>","DOI":"10.1590\/s1806-83242006000100007","type":"journal-article","created":{"date-parts":[[2006,8,9]],"date-time":"2006-08-09T16:40:12Z","timestamp":1155141612000},"page":"33-39","source":"Crossref","is-referenced-by-count":2,"title":["Effects of Tissucol\u2122 and epsilon aminocaproic acid in the healing process following dental extraction in dehydrated rats"],"prefix":"10.1590","volume":"20","author":[{"given":"Tetuo","family":"Okamoto","sequence":"first","affiliation":[{"name":"State University of S\u00e3o Paulo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Cristina Rosifini","family":"Alves-Rezende","sequence":"additional","affiliation":[{"name":"State University of S\u00e3o Paulo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina Chiantelli","family":"Cl\u00e1udio","sequence":"additional","affiliation":[{"name":"State University of S\u00e3o Paulo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tha\u00eds da Silveira","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"State University of S\u00e3o Paulo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberta","family":"Okamoto","sequence":"additional","affiliation":[{"name":"State University of S\u00e3o Paulo"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","first-page":"13","article-title":"Effects of fibrin adhesive material (Tissucol\u00ae) on alveolar healing in rats under stress","volume":"8","author":"Alves-Rezende MCR","year":"1997","journal-title":"Braz Dent J","ISSN":"http:\/\/id.crossref.org\/issn\/0103-6440","issn-type":"print"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1055\/s-0038-1654579","article-title":"The influence of fibrin stabilizing factor on the growth of fibroblasts in vitro and wound healing","volume":"6","author":"Beck E","year":"1961","journal-title":"Thromb Diath Haemorrh","ISSN":"http:\/\/id.crossref.org\/issn\/0340-5338","issn-type":"print"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1055\/s-0029-1201395","article-title":"Uber Wirkungen des Fibrins","volume":"35","author":"Bergel S","year":"1909","journal-title":"Dtsch Med Wochenschr","ISSN":"http:\/\/id.crossref.org\/issn\/0012-0472","issn-type":"print"},{"key":"ref4","first-page":"113","article-title":"Efeitos da desidrata\u00e7\u00e3o sobre a evolu\u00e7\u00e3o da repara\u00e7\u00e3o p\u00f3s-exod\u00f4ntica","volume":"17","author":"Cabrera MA","year":"1988","journal-title":"Rev Odontol Unesp","ISSN":"http:\/\/id.crossref.org\/issn\/0101-1774","issn-type":"print"},{"key":"ref5","first-page":"770","article-title":"Altera\u00e7\u00f5es sang\u00fc\u00edneas induzidas por hidropenia em ratos","volume":"34","author":"Cabrera-Peralta C","year":"1982","journal-title":"Cien Cult"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1001\/jama.1944.02850140022006","article-title":"Use of thrombin and fibrinogen in skin grafting","volume":"124","author":"Cronkite EP","year":"1944","journal-title":"JAMA","ISSN":"http:\/\/id.crossref.org\/issn\/0098-7484","issn-type":"print"},{"key":"ref7","series-title":"Collagen-platelet interaction","first-page":"391","volume-title":"Factor XIII fibrin and collagen","author":"Duckert F","year":"1978"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1177\/153857448301700504","article-title":"Autologous fibrin for tissue-adhesion, hemostasis and embolization","volume":"17","author":"Gestring GF","year":"1983","journal-title":"Vasc Surg","ISSN":"http:\/\/id.crossref.org\/issn\/0042-2835","issn-type":"print"},{"key":"ref9","first-page":"452","article-title":"Fibrin as a hemostatic in cerebral surgery","volume":"21","author":"Grey EG","year":"1915","journal-title":"Surg Gynecol Obstet","ISSN":"http:\/\/id.crossref.org\/issn\/0039-6087","issn-type":"print"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1126\/science.108.2802.280","article-title":"On the solubility of fibrin clots","volume":"108","author":"Laki K","year":"1948","journal-title":"Science","ISSN":"http:\/\/id.crossref.org\/issn\/0036-8075","issn-type":"print"},{"key":"ref11","first-page":"473","article-title":"Effects of dehydration on plasma water and extracellular fluid volumes in the rat","volume":"146","author":"Larsson M","year":"1980","journal-title":"Acta Chir Scand","ISSN":"http:\/\/id.crossref.org\/issn\/0001-5482","issn-type":"print"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1016\/0278-2391(85)90129-6","article-title":"Fibrin seal: the state of the art","volume":"43","author":"Matras H","year":"1985","journal-title":"J Oral Maxillofac Surg","ISSN":"http:\/\/id.crossref.org\/issn\/0278-2391","issn-type":"print"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/0278-2391(82)90108-2","article-title":"The use of fibrin sealant in oral and maxillofacial surgery","volume":"40","author":"Matras H","year":"1982","journal-title":"J Oral Maxillofac Surg","ISSN":"http:\/\/id.crossref.org\/issn\/0278-2391","issn-type":"print"},{"key":"ref14","first-page":"517","article-title":"Zur nachtlosen Interfaszikularen Nerventransplantation in Tierexperiment","volume":"122","author":"Matras H","year":"1972","journal-title":"Wien Med Wochenschr","ISSN":"http:\/\/id.crossref.org\/issn\/0043-5341","issn-type":"print"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1177\/00220345450240030501","article-title":"Formic acid-sodium citrate decalcification and butyl alcohol dehydration of teeth and bone for sectioning in paraffin","volume":"24","author":"Morse A","year":"1945","journal-title":"J Dent Res","ISSN":"http:\/\/id.crossref.org\/issn\/0022-0345","issn-type":"print"},{"key":"ref16","first-page":"36","article-title":"Implante de adesivo fibr\u00ednico (Tissucol\u00ae) em cavidade cir\u00fargica preparada em t\u00edbia de rato: Estudo histol\u00f3gico","volume":"2","author":"Okamoto T","year":"1997","journal-title":"Rev ABO Nac"},{"key":"ref17","first-page":"33","article-title":"Implante da associa\u00e7\u00e3o esponja de fibrina Fibrinol\u00ae\/adesivo fibr\u00ednico (Tissucol\u00ae) em cavidade cir\u00fargica preparada em t\u00edbia de rato: Estudo histol\u00f3gico","volume":"10","author":"Okamoto T","year":"1996","journal-title":"Rev Odontol Univ S\u00e3o Paulo","ISSN":"http:\/\/id.crossref.org\/issn\/0103-0663","issn-type":"print"},{"key":"ref18","first-page":"77","article-title":"Osseous regeneration in the presence of fibrin adhesive material (Tissucol\u00ae) and epsilon-aminocaproic acid (EACA)","volume":"6","author":"Okamoto T","year":"1995","journal-title":"Braz Dent J","ISSN":"http:\/\/id.crossref.org\/issn\/0103-6440","issn-type":"print"},{"key":"ref19","first-page":"12","article-title":"The effect of fibrin sponge (Fibrinol\u00ae), fibrin adhesive (Tissucol\u00ae) and epsilon-aminocaproic acid (EACA) on the healing of experimental rat tibial lesions","volume":"10","author":"Okamoto T","year":"1997","journal-title":"Rev Faculdade Odontol Lins","ISSN":"http:\/\/id.crossref.org\/issn\/0104-7582","issn-type":"print"},{"key":"ref20","first-page":"153","article-title":"Wound healing following tooth extraction: Histochemical study in rats","volume":"2","author":"Okamoto T","year":"1973","journal-title":"Rev Fac Odontol Ara\u00e7atuba"},{"key":"ref21","first-page":"129","article-title":"Glanzmann's thrombasthenia: the use of autologous fibrin glue in tooth extractions","volume":"62","author":"Rakocz M","year":"1995","journal-title":"ASDC J Dent Child","ISSN":"http:\/\/id.crossref.org\/issn\/0022-0353","issn-type":"print"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/0030-4220(93)90135-Q","article-title":"Dental extractions in patients with bleeding disorders: The use of fibrin glue","volume":"75","author":"Rakocz M","year":"1993","journal-title":"Oral Surg Oral Med Oral Pathol","ISSN":"http:\/\/id.crossref.org\/issn\/0030-4220","issn-type":"print"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1056\/NEJM199506223322501","article-title":"A comparison of six weeks with six months of oral anticoagulant therapy after a first episode of venous thromboembolism Duration of anticoagulation trial study group.","volume":"332","author":"Schulman S","year":"1995","journal-title":"N Engl J Med","ISSN":"http:\/\/id.crossref.org\/issn\/0028-4793","issn-type":"print"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1056\/NEJM198903303201305","article-title":"Hemostatic effect of tranexamic acid mouthwash in anticoagulant-treated patients undergoing oral surgery","volume":"320","author":"Sindet-Pedersen S","year":"1989","journal-title":"N Engl J Med","ISSN":"http:\/\/id.crossref.org\/issn\/0028-4793","issn-type":"print"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1177\/000348947908800320","article-title":"Tissue adhesion with highly concentrated human fibrinogen in otolaryngology","volume":"88","author":"Staindl O","year":"1979","journal-title":"Ann Otol Rhinol Laryngol","ISSN":"http:\/\/id.crossref.org\/issn\/0003-4894","issn-type":"print"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/S0140-6736(01)07978-8","article-title":"Fibrin suture of peripheral 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