{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T03:50:01Z","timestamp":1790049001975,"version":"4.0.1"},"reference-count":29,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:p>In this study, we have investigated the incidence of transplacental transmission of Anaplasma marginale in chronically infected cows with no history of acute anaplasmosis during gestation. In addition, we evaluated various techniques for detection of transplacental transmission ofA. marginale. Blood samples were collected from 30 cows at three different periods: at the time of artificial insemination, at gestational diagnosis, and after calving. Also, blood was collected from the newborn calves, including one sample before colostrum intake, and another three days after birth. A. marginale-specific antibodies were detected in 100% of the cows with an indirect fluorescent antibody test (IFAT), and in 97% of them, using an indirect enzyme-linked immunosorbent assay (ELISA). Also, we observed that all of the three-day-old newborn calves were seropositive by IFAT. According to polymerase chain reaction, 63.3% of the cows were carriers of A. marginale, as well as 6.7% of the newborn calves. This represented a transplacental transmission rate of 10.5%. Furthermore, a correlation of 93.3% was observed between the two serodiagnostic techniques, demonstrating that both ELISA and IFAT can be used in epidemiological surveys of A. marginale. These results confirm the occurrence of transplacental transmission of A. marginale in chronically infected cows and suggest the importance of this transmission route in areas of enzootic instability.<\/jats:p>","DOI":"10.1590\/s1984-29612013000200038","type":"journal-article","created":{"date-parts":[[2014,2,27]],"date-time":"2014-02-27T16:06:06Z","timestamp":1393517166000},"page":"189-193","source":"Crossref","is-referenced-by-count":37,"title":["Transplacental transmission of Anaplasma marginale in beef cattle chronically infected in southern Brazil"],"prefix":"10.1590","volume":"22","author":[{"given":"Hermann Eduardo Gonzalez","family":"Grau","sequence":"first","affiliation":[{"name":"Universidade Federal de Pelotas - UFPel, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilton Azevedo da","family":"Cunha Filho","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas - UFPel, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe Geraldo","family":"Pappen","sequence":"additional","affiliation":[{"name":"Instituto Federal Catarinense - IFC, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nara Am\u00e9lia da Rosa","family":"Farias","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas - UFPel, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"key":"ref1","first-page":"29","article-title":"<em>Clonagem e express\u00e3o do gene msp5 de um isolado brasileiro de Anaplasma marginale<\/em>. Campo Grande: Embrapa Gado de Corte; 2002","author":"Ara\u00fajo FR","journal-title":"Circular T\u00e9cnica n"},{"key":"ref2","first-page":"179","article-title":"Preval\u00eancia de <em>Babesia bovis, Babesia bigemina<\/em> e <em>Anaplasma marginale<\/em> no munic\u00edpio de Bag\u00e9, RS","volume":"(S1)","author":"Artiles J","year":"1995","journal-title":"Braz J Vet Parasitol"},{"issue":"(5)","key":"ref3","first-page":"543","article-title":"Comparison between ELISA and Indirect Fluorescent Antibody Test in the detection of antibodies against <em>Anaplasma marginale<\/em> in cattle","volume":"49","author":"Braz CJ Jr","year":"1997","journal-title":"Arq Bra Med Vet Zootec"},{"issue":"(4)","key":"ref4","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1046\/j.1439-0450.2000.00342.x","article-title":"Development of an ELISA System for detection of anti-<em>Anaplasma marginale<\/em> antibodies in cattle in Brazil","volume":"47","author":"Braz CJ Jr","year":"2000","journal-title":"J Vet Med B Infect Dis Vet Public Health"},{"key":"ref5","first-page":"49","article-title":"<em>Epidemiologia molecular de Anaplasma marginale em bovinos criados nos estados de Rond\u00f4nia e Acre<\/em>. Porto Velho: Embrapa Rond\u00f4nia; 2007","author":"Brito LG","journal-title":"Boletim de Pesquisa e Desenvolvimento n"},{"key":"ref6","series-title":"Epidemiology for the Uninitiated","first-page":"20","volume-title":"Measurement, error and bias","author":"Coggon T","year":"1993","edition":"3rd ed."},{"issue":"(4)","key":"ref7","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1099\/00207713-51-6-2145","article-title":"Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of <em>Ehlrlichia<\/em> with<em>Anaplasma<\/em>, <em>Cowdria<\/em> with<em>Ehrlichia<\/em> and <em>Ehrlichia<\/em> with<em>Neorickettsia<\/em>, descriptions of six new species combinations and designation of <em>Ehrlichia equi<\/em> and \u2018HGE agent\u2019 as subjective synonyms of <em>Ehrlichia phagocytophila<\/em>","volume":"51","author":"Dumler JS","year":"2001","journal-title":"Int J Syst Evol Microbiol"},{"issue":"(1)","key":"ref8","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1590\/S0103-84782000000100030","article-title":"Epidemiologia e controle da tristeza parasit\u00e1ria bovina na regi\u00e3o sudeste do Brasil","volume":"30","author":"Gon\u00e7alves PM","year":"2000","journal-title":"Cienc Rural"},{"issue":"(4)","key":"ref9","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1590\/S0100-736X2001000400009","article-title":"Considera\u00e7\u00f5es sobre a transmiss\u00e3o de<em>Anaplasma marginale<\/em>","volume":"21","author":"Kessler RH","year":"2001","journal-title":"Pesq Vet Bras"},{"key":"ref10","first-page":"1225","article-title":"Babesiose cerebral por <em>Babesia bovis<\/em> (Babes, 1888, Starcovici, 1983) em bezerros do estado do Mato Grosso do Sul","volume":"22","author":"Kessler RH","year":"1983","journal-title":"Pesq Agropec Bras"},{"issue":"(1)","key":"ref11","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1111\/j.1749-6632.2000.tb05329.x","article-title":"Anaplasmosis control: past, present and future","volume":"916","author":"Kocan KM","year":"2000","journal-title":"Ann N Y Acad Sci"},{"issue":"(1)","key":"ref12","doi-asserted-by":"crossref","first-page":"12","DOI":"10.7589\/0090-3558-20.1.12","article-title":"<em>Anaplasma<\/em> infections in wild and domestic ruminants: a review","volume":"20","author":"Kuttler KL","year":"1984","journal-title":"J Wildl Dis"},{"issue":"(3)","key":"ref13","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1590\/S0100-736X2000000300004","article-title":"Evaluation of an enzyme-linked immunosorbent assay to detect antibodies against <em>Anaplasma marginale<\/em>","volume":"20","author":"Madruga CR","year":"2000","journal-title":"Pesq Vet Bras"},{"key":"ref14","article-title":"<em>Imunodiagn\u00f3stico em Medicina Veterin\u00e1ria<\/em>. Campo Grande: Embrapa Gado de Corte; 2001","author":"Madruga CR","journal-title":"369 p"},{"issue":"(5","key":"ref15","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1111\/j.1751-0813.1972.tb05160.x","article-title":"Epizootiological factors in the control of bovine babesiosis","volume":"48)","author":"Mahoney DF","year":"1972","journal-title":"Aust Vet J"},{"issue":"(2)","key":"ref16","doi-asserted-by":"crossref","first-page":"5","DOI":"10.4322\/rbpv.01802002","article-title":"Clonagem, express\u00e3o, caracteriza\u00e7\u00e3o molecular da prote\u00edna de superf\u00edcie MSP 5 da amostra PR1 de <em>Anaplasma marginale<\/em> e sua aplica\u00e7\u00e3o em um teste de ELISA por competi\u00e7\u00e3o","volume":"18","author":"Marana ERM","year":"2009","journal-title":"Braz J Vet Parasitol"},{"issue":"(3)","key":"ref17","first-page":"179","article-title":"Fatal congenital anaplasmosis associated with bovine viral diarrhoea virus (BVDV) infection in a cossbreed calf","volume":"82","author":"Pypers AR","year":"2011","journal-title":"JSAVA"},{"issue":"(4)","key":"ref18","first-page":"557","article-title":"The persistence of colostral <em>Anaplasma marginale<\/em> antibodies and incidence of in utero transmission of<em>Anaplasma<\/em> infections in calves under laboratory conditions","volume":"54","author":"Potgieter FT","year":"1987","journal-title":"Onderstepoort J Vet Res"},{"key":"ref19","series-title":"530 p","volume-title":"<em>Diseases of Dairy Cattle<\/em>","author":"Rehbun WC","year":"1995"},{"issue":"(1)","key":"ref20","first-page":"63","article-title":"Natural exposure of calves to <em>Anaplasma marginale<\/em> in endemic areas of Minas Gerais","volume":"33","author":"Ribeiro MFB","year":"1981","journal-title":"Arq Esc Vet UFMG"},{"issue":"(3)","key":"ref21","first-page":"297","article-title":"Transmiss\u00e3o cong\u00eanita da anaplasmose bovina","volume":"47","author":"Ribeiro MFB","year":"1995","journal-title":"Arq Bras Med Vet Zootec"},{"issue":"(1-2)","key":"ref22","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/0304-4017(95)00800-4","article-title":"Morphology and development of <em>Anaplasma marginale<\/em> in midgut of engorged female ticks of<em>Boophilus microplus<\/em>","volume":"61","author":"Ribeiro MFB","year":"1996","journal-title":"Vet Parasitol"},{"key":"ref23","article-title":"<em>Anaplasma marginale (THEILER, 1910)<\/em>: Imunidade passiva e ativa em bovinos nascidos na primavera no sul do Rio Grande do Sul [Disserta\u00e7\u00e3o]","author":"Silva GAC","year":"2003","journal-title":"Pelotas: Universidade Federal de Pelotas"},{"key":"ref24","author":"Smith RD","year":"1995"},{"key":"ref25","series-title":"Madruga CR, Ara\u00fajo FR, Soares CO. Imunodiagn\u00f3stico em Medicina Veterin\u00e1ria","first-page":"145","volume-title":"Princ\u00edpios, padroniza\u00e7\u00e3o e valida\u00e7\u00e3o de provas sorol\u00f3gicas","author":"Soares CO","year":"2001"},{"key":"ref26","article-title":"Statistix 9. Analytical software","year":"2008","journal-title":"Tallahassee"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1111\/j.1749-6632.1992.tb19625.x","article-title":"Biotechnology: a new approach to the diagnosis and control of tick-borne hemoparasitic diseases","volume":"653","author":"Stiller D","year":"1992","journal-title":"Ann N Y Acad Sci"},{"issue":"(6)","key":"ref28","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/0093-691X(83)90192-9","article-title":"Anestrus in cycling beef heifers experimentally inoculated with <em>Anaplasma marginale<\/em>","volume":"20","author":"Swift BL","year":"1983","journal-title":"Theriogenology"},{"issue":"(3)","key":"ref29","doi-asserted-by":"crossref","first-page":"440","DOI":"10.2460\/ajvr.1984.45.03.440","article-title":"Bovine anaplasmosis: <em>in utero<\/em>transmission and the immunologic significance of ingested colostral antibodies","volume":"45","author":"Zaugg JL","year":"1984","journal-title":"Am J Vet Res"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rbpv\/v22n2\/0103-846X-rbpv-22-02-189.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T23:19:47Z","timestamp":1746141587000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612013000200189&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["S1984-29612013000200189"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612013000200038","relation":{},"ISSN":["1984-2961"],"issn-type":[{"value":"1984-2961","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6]]}}}