{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T16:25:43Z","timestamp":1784305543822,"version":"3.55.0"},"reference-count":8,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:p>There is little information on the humoral response of sheep experimentally infected with Echinococcus granulosus. Thus, the objective of this study was to evaluate this response and measure its evolution. Doses of 10, 100, 1000 and 10000 E. granulosus eggs were prepared and inoculated via intraruminal puncture. Blood samples were obtained before inoculation and every 48 h after inoculation, until they became seropositive. Thereafter, they were taken monthly for the first year and then every three months until 1700 days of observation had been completed. An ELISA test, with total hydatid fluid antigen, was used for immunodiagnosis. The average optical density of the 12 inoculated sheep was found to be above the mean cutoff value 10 days after inoculation, went on increasing until 180 days after inoculation and remained above the cutoff level until the end of the observation period. This confirms that the antibody response of sheep to E. granulosus infection occurs before production of hydatid fluid and that activation, mobilization and establishment of oncospheres in the tissues generates a persistent response from the host's immune system.<\/jats:p>","DOI":"10.1590\/s1984-29612014030","type":"journal-article","created":{"date-parts":[[2014,7,17]],"date-time":"2014-07-17T09:31:28Z","timestamp":1405589488000},"page":"237-240","source":"Crossref","is-referenced-by-count":5,"title":["Humoral response and evolution of Echinococcus infection in experimentally infected sheep"],"prefix":"10.1590","volume":"23","author":[{"given":"Roberto","family":"Lamberti","sequence":"first","affiliation":[{"name":"Universidad Nacional de la Pampa,  Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Cavagion","sequence":"additional","affiliation":[{"name":"Universidad Nacional de la Pampa,  Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Gatti","sequence":"additional","affiliation":[{"name":"Universidad Nacional de R\u00edo Negro,  Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Calvo","sequence":"additional","affiliation":[{"name":"Universidad Nacional de la Pampa,  Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilia","family":"Gino","sequence":"additional","affiliation":[{"name":"Universidad Nacional de la Pampa,  Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronica V\u00e9spoli","family":"Puches","sequence":"additional","affiliation":[{"name":"Universidad Nacional de la Pampa,  Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela Rosa","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Universidad Nacional de la Pampa,  Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emiliano","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Universidad Nacional de la Pampa,  Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariela Garc\u00eda","family":"Cachau","sequence":"additional","affiliation":[{"name":"Universidad Nacional de la Pampa,  Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariela","family":"Morete","sequence":"additional","affiliation":[{"name":"Universidad Nacional de la Pampa,  Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edmundo","family":"Larrieu","sequence":"additional","affiliation":[{"name":"Universidad Nacional de la Pampa,  Argentina; Universidad Nacional de R\u00edo Negro,  Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"issue":"(4)","key":"ref1","doi-asserted-by":"crossref","first-page":"577","DOI":"10.2307\/3280512","article-title":"Immunodiagnostic tests for hydatidosis in sheep: an evaluation of double diffusion, immunoelectrophoresis, indirect hemagglutination, and intradermal tests","volume":"66","author":"Conder GA","year":"1980","journal-title":"J Parasitol"},{"key":"ref2","first-page":"72","article-title":"Echinococcosis in animals: clinical aspects, diagnosis and treatment","author":"Eckert J","year":"2001","journal-title":"WHO\/OIE manual on Echinococcosis in Humans and Animals: a public health problem of global concern"},{"issue":"(2)","key":"ref3","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.vetpar.2006.08.022","article-title":"Ovine Echinococcosis: I. 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Aires.)"},{"issue":"(1)","key":"ref6","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1590\/S1020-49892012000100012","article-title":"Critical analysis of cystic echinococcosis control programs and praziquantel use in South America, 1974-2010","volume":"31","author":"Larrieu E","year":"2012","journal-title":"Rev Panam Salud P\u00fablica"},{"key":"ref7","first-page":"355","article-title":"Echinococcosis\/Hydatidosis: antigens, immunological and molecular diagnosis","author":"Lightowlers M","year":"1995","journal-title":"The biology of Echinococcus granulosus and hydatid disease"},{"key":"ref8","first-page":"1","article-title":"Aetiology: parasites and life cycles","author":"Thompson RCA","year":"2001","journal-title":"WHO\/OIE manual on Echinococcosis in Humans and Animals: a public health problem of global concern"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rbpv\/v23n2\/0103-846X-rbpv-23-02-237.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T01:18:49Z","timestamp":1495502329000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612014000200237&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6]]},"references-count":8,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["S1984-29612014000200237"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612014030","relation":{},"ISSN":["1984-2961"],"issn-type":[{"value":"1984-2961","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6]]}}}