{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T05:18:38Z","timestamp":1768972718411,"version":"3.49.0"},"reference-count":37,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:p>The aim of the present study was to evaluate the serological methods using ELISA with recombinant-rK39 (ELISA-rK-39) and soluble extract-SE (ELISA-SE) antigens, the indirect fluorescence antibody test (IFAT) in comparison to an immunochromatography rapid diagnostic test (RDT-rK39) and with a direct parasitological exam (PA) for Canine Visceral Leishmaniasis (CVL) diagnosis. The results showed that 89% (60\/67) of the dogs were positive for at least one serological diagnostic test. ELISA-SE was the test that detected anti-Leishmania antibodies in the serum of the highest number of dogs (71.6%) followed by ELISA-rK39 (65.7%), IFAT (65.7%) and RDT-rK39 (55.2%). PA detected the lowest numbers (40.3%) of positive dogs. In relation to the total of examined dogs, the Kappa indexes (p \u2264 0.05) showed a good agreement between ELISA-SE and IFAT (88.1%; k = 0.7237), and it was also observed in the comparison of RDT-rK39 with ELISA-SE (83.6%, k= 0.6561), IFAT (83.5%, k= 0.6605) and PA (85.0%, k= 0.7074). A bad agreement was detected in any association of ELISA-rk39 with the other tests in either symptomatic or asymptomatic animals. ELISA as well as RDT using recombinant antigenic protein (rK39) were the methods that detected the lowest prevalence rates (33.3%) of CVL in asymptomatic dogs. In conclusion, only one test does not adequately identify dogs with CVL and it is necessary the association of two or more diagnostic tests. Because of the good agreement indexes of RDT-rK39 when evaluated with ELISA-SE, IFAT and PA it was suggested as a complementary method to be used in association with either ELISA-SE or IFAT, particularly in the symptomatic dogs. Furthermore, new studies are recommended in order to improve the sensitivity of tests mainly for asymptomatic dogs.<\/jats:p>","DOI":"10.1590\/s1984-29612014033","type":"journal-article","created":{"date-parts":[[2014,5,22]],"date-time":"2014-05-22T09:40:02Z","timestamp":1400751602000},"page":"179-186","source":"Crossref","is-referenced-by-count":13,"title":["Comparative evaluation of several methods for Canine Visceral Leishmaniasis diagnosis"],"prefix":"10.1590","volume":"23","author":[{"given":"Diogo Tiago da","family":"Silva","sequence":"first","affiliation":[{"name":"Universidade Estadual Paulista \u2013 UNESP, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilma Aparecida","family":"Starke-Buzetti","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista \u2013 UNESP, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Fernanda","family":"Alves-Martin","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista \u2013 UNESP, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirian dos Santos","family":"Paix\u00e3o","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista \u2013 UNESP, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michely da Silva","family":"Ten\u00f3rio","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista \u2013 UNESP, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mara L\u00facia Martins","family":"Lopes","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista \u2013 UNESP, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0065-308X(04)57001-X","article-title":"Canine leishmaniasis","volume":"57","author":"Alvar J","year":"2004","journal-title":"Adv Parasitol"},{"issue":"(1)","key":"ref2","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1590\/S1984-29612010000100005","article-title":"Estudo comparativo dos m\u00e9todos diagn\u00f3sticos para Leishmaniose Visceral em c\u00e3es oriundos de Ilha Solteira, SP. 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