{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T23:15:40Z","timestamp":1762298140922},"reference-count":14,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:p>Leishmaniasis is a zoonotic disease caused by protozoa of the genus Leishmania (Ross, 1903) and is the focus of considerable attention in human and veterinary medicine. In the city of Campo Grande, MS, the causative agent of visceral leishmaniasis is Leishmania infantum (= L. chagasi) primary vector, comprising approximately 92.9% of the local sandfly population, is Lutzomyia longipalpis. The aim of this work was to compare real-time PCR with PCR as a tool for the detection of the kinetoplast DNA (kDNA) of L. infantum in sandflies. Sandflies of this species were caught, and a total of 38 samples with 1-4 individuals in each sample were obtained; these were distributed across 13 districts and divided between seven urban areas of the city of Campo Grande, MS. Three positive samples were found by PCR and, when using real-time PCR, this was able to detect the presence of this agent in 6 of the 13 districts sampled, which were all located on the outskirts of the city, where indicates the greater enzootic potential of these regions, as they are closer to natural forest reserves. We conclude that real-time PCR can be used for epidemiological studies of L. infantum.<\/jats:p>","DOI":"10.1590\/s1984-29612014049","type":"journal-article","created":{"date-parts":[[2014,7,17]],"date-time":"2014-07-17T13:31:22Z","timestamp":1405603882000},"page":"269-273","source":"Crossref","is-referenced-by-count":17,"title":["Detection of Leishmania infantum in Lutzomyia longipalpis captured in Campo Grande, MS"],"prefix":"10.1590","volume":"23","author":[{"given":"Rodrigo Casquero","family":"Cunha","sequence":"first","affiliation":[{"name":"Universidade Federal de Pelotas \u2013 UFPel,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato","family":"Andreotti","sequence":"additional","affiliation":[{"name":"Embrapa Gado de Corte,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marlon Cezar","family":"Cominetti","sequence":"additional","affiliation":[{"name":"Universidade Federal de Mato Grosso do Sul \u2013 UFMS,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaine Ara\u00fajo","family":"Silva","sequence":"additional","affiliation":[{"name":"Secretaria Municipal de Sa\u00fade,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","article-title":"Prefeitura Municipal. Instituto Municipal de Planejamento Urbano - PLANURB","author":"Campo Grande","year":"2013","journal-title":"Perfil S\u00f3cio-Econ\u00f4mico de Campo Grande"},{"issue":"(3)","key":"ref2","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1016\/j.tvjl.2006.11.006","article-title":"Canine visceral leishmaniasis: relationships between clinical status, humoral immune response, haematology and Lutzomyia (Lutzomyia) longipalpisinfectivity","volume":"174","author":"Costa-Val AP da","year":"2007","journal-title":"Vet J"},{"issue":"(4)","key":"ref3","first-page":"17","article-title":"Laboratory diagnosis and clinical signs of canine visceral leishmaniasis in dogs examined at the center for zoonosis control in Campo Grande - MS, Brazil","volume":"17","author":"Cunha R","year":"2012","journal-title":"Arch Vet Sci"},{"issue":"(1)","key":"ref4","first-page":"43","article-title":"Ocorr\u00eancia de leishmaniose tegumentar americana no Estado do Mato Grosso do Sul associada \u00e0 infec\u00e7\u00e3o por Leishmania (Leishmania) amazonensis","volume":"39","author":"Dorval MEMC","year":"2013","journal-title":"Rev Soc Bras Med Trop"},{"issue":"(3)","key":"ref5","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.vetpar.2006.10.008","article-title":"PCR identification of Leishmaniain diagnosis and control of canine leishmaniasis","volume":"144","author":"Gomes AHS","year":"2007","journal-title":"Vet Parasitol"},{"issue":"(3)","key":"ref6","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1590\/S1415-790X2004000300011","article-title":"Leishmaniose visceral no Brasil: quadro atual, desafios e perspectivas","volume":"7","author":"Gontijo CMF","year":"2004","journal-title":"Rev Bras Epidemiol"},{"issue":"(2)","key":"ref7","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S0001-706X(00)00141-8","article-title":"Experimental infection of Phlebotomus perniciosus and determination of the natural infection rates of Leishmania infantum in dogs","volume":"77","author":"Guarga JL","year":"2000","journal-title":"Acta Trop"},{"key":"ref8","year":"2006"},{"issue":"(1)","key":"ref9","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1128\/JCM.40.1.210-215.2002","article-title":"Comparison of six PCR methods using peripheral blood for detection of canine visceral leishmaniasis","volume":"40","author":"Lachaud L","year":"2002","journal-title":"J Clin Microbiol"},{"issue":"(3)","key":"ref10","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1590\/S0037-86822009000300012","article-title":"Identifica\u00e7\u00e3o de esp\u00e9cies de Leishmania isoladas de casos humanos em Mato Grosso do Sul por meio da rea\u00e7\u00e3o em cadeia da polimerase","volume":"42","author":"Lima Jr MSC","year":"2009","journal-title":"Rev Soc Bras Med Trop"},{"issue":"(4)","key":"ref11","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/0035-9203(94)90446-4","article-title":"Infectivity of dogs naturally infected with Leishmania infantum to colonized Phlebotomus perniciosus","volume":"88","author":"Molina R","year":"1994","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"(3)","key":"ref12","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1590\/S0037-86821995000300004","article-title":"Estudo epidemiol\u00f3gico sobre Leishmaniose tegumentar (LT) no munic\u00edpio de Corguinho, Mato Grosso do Sul: estudos na popula\u00e7\u00e3o humana","volume":"28","author":"Nunes VLB","year":"1995","journal-title":"Rev Soc Bras Med Trop"},{"issue":"(2-3)","key":"ref13","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.actatropica.2006.08.009","article-title":"Detection and identification of Leishmania species in field-captured phlebotomine sandflies based on mini-exon gene PCR","volume":"99","author":"Paiva BR","year":"2006","journal-title":"Acta Trop"},{"issue":"(3)","key":"ref14","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.exppara.2008.03.011","article-title":"Detection of Leishmania DNA in phlebotomines captured in Campo Grande, Mato Grosso do Sul, Brazil","volume":"119","author":"Silva EA","year":"2008","journal-title":"Exp Parasitol"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rbpv\/v23n2\/0103-846X-rbpv-23-02-269.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T05:20:35Z","timestamp":1495516835000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612014000200269&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6]]},"references-count":14,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["S1984-29612014000200269"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612014049","relation":{},"ISSN":["1984-2961"],"issn-type":[{"value":"1984-2961","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6]]}}}