{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T08:44:14Z","timestamp":1776847454160,"version":"3.51.2"},"reference-count":52,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Blood samples were collected from 99 domestic dogs from the urban and rural areas of the L\u00e1brea municipality, state of Amazonas, Brazil. Canine serum samples were tested by immunofluorescence assay against Rickettsia spp., which revealed that only 3.0% (1\/33) and 7.6% (5\/66) of the dogs from urban and rural areas, respectively, reacted positively to at least one Rickettsia species. DNA was extracted from canine blood and tested by a battery of PCR assays targeting protozoa of the genera Babesia and Hepatozoon, and bacteria of the genera Rickettsia and Ehrlichia and family Anaplasmataceae. All samples were negative in the PCR assays targeting the genera Babesia, Hepatozoon, Ehrlichia and Rickettsia. For Anaplasmataceae, 3% (1\/33) and 39.4% (26\/66) of the urban and rural dogs, respectively, yielded amplicons that generated DNA sequences 100% identical to the corresponding sequence of Wolbachia endosymbiont of Dirofilaria immitis. Because of these results, all canine DNA samples were further tested in a PCR assay targeting filarial nematodes, which was positive for 18.2% (6\/33) and 57.6% (38\/66) urban and rural dogs, respectively. Filarial-PCR products generated DNA sequences 100% identical to D. immitis. While tick-borne infections were rare in L\u00e1brea, D. immitis infection rates were among the highest reported in South America.<\/jats:p>","DOI":"10.1590\/s1984-29612014093","type":"journal-article","created":{"date-parts":[[2014,12,18]],"date-time":"2014-12-18T15:51:21Z","timestamp":1418917881000},"page":"473-480","source":"Crossref","is-referenced-by-count":23,"title":["Survey of canine tick-borne diseases in L\u00e1brea, Brazilian Amazon: \u2018accidental\u2019 findings of Dirofilaria immitis infection"],"prefix":"10.1590","volume":"23","author":[{"given":"Herbert Sousa","family":"Soares","sequence":"first","affiliation":[{"name":"Universidade de S\u00e3o Paulo \u2013 USP, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Marcelo Aranha","family":"Camargo","sequence":"additional","affiliation":[{"name":"Faculdades S\u00e3o Lucas \u2013 FSL, Brasil; Universidade de S\u00e3o Paulo \u2013 USP, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Solange Maria","family":"Gennari","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo \u2013 USP, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo Bahia","family":"Labruna","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo \u2013 USP, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2014,12]]},"reference":[{"issue":"(1)","key":"ref1","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1093\/jmedent\/41.5.126","article-title":"Prevalence of Ehrlichia canis (Rickettsiales: Anaplasmataceae) in dogs and Rhipicephalus sanguineus (Acari: Ixodidae) ticks from Brazil","volume":"44","author":"Aguiar DM","year":"2007","journal-title":"J Med 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