{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T01:05:23Z","timestamp":1774314323796,"version":"3.50.1"},"reference-count":46,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:p>Abstract The objective of the study was to report on a fatal case of feline toxoplasmosis with coinfection with the feline leukemia virus (FeLV). A domestic cat (Felis silvestris catus) presented intense dyspnea and died three days later. In the necropsy, the lungs were firm, without collapse and with many white areas; moderate lymphadenomegaly and splenomegaly were also observed. The histopathological examination showed severe necrotic interstitial bronchopneumonia and mild necrotic hepatitis, associated with intralesional cysts and tachyzoites of Toxoplasma gondii that were positive by anti-T. gondii immunohistochemical (IHC) evaluation. The bone marrow showed chronic myeloid leukemia and the neoplastic cells were positive by anti-FeLV IHC evaluation. DNA extracted from lungs was positive for T. gondii by PCR targeting REP-529. T. gondii was characterized by PCR-RFLP and by the microsatellites technique. ToxoDB-PCR-RFLP #10, i.e. the archetypal type I, was identified. Microsatellite analysis showed that the strain was a variant of type I with two atypical alleles. This was the first time that a T. gondii clonal type I genotype was correlated with a case of acute toxoplasmosis in a host in Brazil.<\/jats:p>","DOI":"10.1590\/s1984-29612017025","type":"journal-article","created":{"date-parts":[[2017,7,19]],"date-time":"2017-07-19T18:13:16Z","timestamp":1500487996000},"page":"177-184","source":"Crossref","is-referenced-by-count":17,"title":["Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I"],"prefix":"10.1590","volume":"26","author":[{"given":"Hilda F\u00e1tima de Jesus","family":"Pena","sequence":"first","affiliation":[{"name":"Universidade de S\u00e3o Paulo, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila Mariellen","family":"Evangelista","sequence":"additional","affiliation":[{"name":"Universidade do Estado de Santa Catarina, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata 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multiplex PCR assay","volume":"48","author":"Ajzenberg D","year":"2010","journal-title":"J Clin Microbiol"},{"issue":"8","key":"ref4","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.2460\/javma.1999.215.08.1123","article-title":"Acute toxoplasmosis following renal transplantation in three cats and a dog","volume":"215","author":"Bernsteen L","year":"1999","journal-title":"J Am Vet Med Assoc"},{"issue":"3","key":"ref5","first-page":"233","article-title":"Phylogenetic analysis: models and estimation procedures","volume":"19","author":"Cavalli-Sforza LL","year":"1967","journal-title":"Am J Hum Genet"},{"issue":"5","key":"ref6","first-page":"483","article-title":"Fatal extraintestinal toxoplasmosis in a young male cat with enlarged mesenteric lymph nodes","volume":"57","author":"Cohen TM","year":"2016","journal-title":"Can Vet J"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1590\/S1984-29612011000300016","article-title":"Seroprevalence of Toxoplasma gondii infection in cats from Curitiba, Paran\u00e1, Brazil","volume":"20","author":"Cruz MA","year":"2011","journal-title":"Rev Bras Parasitol Vet"},{"issue":"5","key":"ref8","first-page":"1486","article-title":"Feline immunodeficiency virus predisposes cats to acute generalized toxoplasmosis","volume":"143","author":"Davidson MG","year":"1993","journal-title":"Am J Pathol"},{"issue":"21","key":"ref9","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1136\/vr.151.21.626","article-title":"Serological survey of Toxoplasma gondii, feline immunodeficiency virus and feline leukaemia virus in urban stray cats in Belgium","volume":"151","author":"Dorny P","year":"2002","journal-title":"Vet Rec"},{"issue":"1-2","key":"ref10","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.vetpar.2006.04.037","article-title":"Prevalence of Toxoplasma gondii in cats from Colombia, South America and genetic characterization of T. gondii isolates","volume":"141","author":"Dubey 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feline leukemia virus infections in cats from Grenada, west indies","volume":"95","author":"Dubey JP","year":"2009","journal-title":"J Parasitol"},{"key":"ref14","series-title":"Toxoplasmosis of animals and humans","author":"Dubey JP","year":"2010","edition":"2nd"},{"key":"ref15","series-title":"Schalm\u2019s veterinary hematology","author":"Feldman BF","year":"2000","edition":"5th"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1093\/nar\/gkm981","article-title":"ToxoDB: an integrated Toxoplasma gondii database resource","volume":"36","author":"Gajria B","year":"2007","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"ref17","first-page":"145","article-title":"Ocorr\u00eancia de gatos soropositivos para Toxoplasma gondii Nicolle e Manceaux, 1909 (Apicomplexa: Toxoplasmatinae) na cidade de Niter\u00f3i, Rio de Janeiro","volume":"12","author":"Gon\u00e7alves No E","year":"2003","journal-title":"Rev Bras Parasitol 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