{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T06:07:40Z","timestamp":1789711660114,"version":"4.0.1"},"reference-count":29,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract The lymnaeids are important in the epidemiology of Fasciola hepatica, a neglected and endemic zoonosis. The interaction between the internal defense system of Pseudosuccinea columella and F. hepatica has been little studied. In the present study the effect of infection by F. hepatica on P. columella circulating haemocytes was investigated. Changes in the average number of total circulating haemocytes have been observed at 30 minutes post-infection and 1, 7, 10, 14, 21, 28 and 50 days post-infection (dpi). Miracidia were observed head-foot and mantle at 30 minutes post-infection. Miracidia\/Sporocysts in the mantle skirt 1 dpi, and fully formed sporocysts were observed in the head-foot at 7 dpi. Rediae became evident at 10 dpi and were located between the haemocoel and the muscles from 14 dpi; 50 dpi, the rediae in the digestive gland contained cercariae. The statistical analysis of the total haemocytes of P. columella infected by F. hepatica showed significant differences on the 30 minutes post-infection and 1, 14, 21, and 28 dpi in comparison to uninfected molluscs (0 dpi). Therefore, the interference observed on the internal defence system of P. columella may have direct association with the development of F. hepatica.<\/jats:p>","DOI":"10.1590\/s1984-29612017057","type":"journal-article","created":{"date-parts":[[2017,11,8]],"date-time":"2017-11-08T22:44:02Z","timestamp":1510181042000},"page":"411-418","source":"Crossref","is-referenced-by-count":8,"title":["Dynamics of haemocytes from Pseudosuccinea columella circulating infected by Fasciola hepatica"],"prefix":"10.1590","volume":"26","author":[{"given":"Vinicius Marques Antunes","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Universidade Federal de Minas Gerais, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aytube Lucas","family":"Coaglio","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando Luiz Pereira","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Federal de Ouro Preto, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00edntia Aparecida de Jesus","family":"Pereira","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter dos Santos","family":"Lima","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2017,11,9]]},"reference":[{"issue":"5","key":"ref1","doi-asserted-by":"crossref","first-page":"719","DOI":"10.2307\/3283251","article-title":"A time-lapse study of interactions between Echinostoma paraensei intramolluscan larval stages and adherent hemocytes from Biomphalaria glabrata and Helix aspersa.","volume":"80","author":"Adema CM","year":"1994","journal-title":"J Parasitol"},{"issue":"2-3","key":"ref2","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/0304-4017(86)90115-9","article-title":"Epidemiology of Fasciola hepatica infection in the Para\u00edba river valley, S\u00e3o Paulo, Brasil","volume":"22","author":"Amato SB","year":"1986","journal-title":"Vet Parasitol"},{"issue":"3","key":"ref3","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0145-305X(91)90001-F","article-title":"Effects of Trichobilharzia ocellata on hemocytes of Lymnaea stagnalis.","volume":"15","author":"Amen RI","year":"1991","journal-title":"Dev Comp Immunol"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.actatropica.2006.01.003","article-title":"Genetic variability of Brazilian populations of Lymnaea columella (Gastropoda: Lymnaeidae), an intermediate host of Fasciola hepatica (Trematoda: Digenea)","volume":"97","author":"Cardoso PC","year":"2006","journal-title":"Acta Trop"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/1756-3305-7-66","article-title":"New insight in lymnaeid snails (Mollusca, Gastropoda) as intermediate hosts of Fasciola hepatica (Trematoda, Digenea) in Belgium and Luxembourg","volume":"7","author":"Caron Y","year":"2014","journal-title":"Parasit Vectors"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1017\/S0022149X08873579","article-title":"Influence of shell size of Lymnaea columella on infectivity and development of Fasciola hepatica.","volume":"82","author":"Coelho LH","year":"2008","journal-title":"J Helminthol"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1017\/S0022149X09222930","article-title":"Sympatric and allopatric combinations of Lymnaea columella and Fasciola hepatica from southern and south-eastern Brazil","volume":"83","author":"Coelho LH","year":"2009","journal-title":"J Helminthol"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.fsi.2015.01.036","article-title":"Advances in gastropod immunity from the study of the interaction between the snail Biomphalaria glabrata and its parasites: A review of research progress over the last decade","volume":"46","author":"Coustau C","year":"2015","journal-title":"Fish Shellfish Immunol"},{"issue":"3","key":"ref9","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1007\/s00436-003-0856-z","article-title":"Fasciola hepatica: the development of tissue lesions and their frequencies in naturally-infected Galba truncatula.","volume":"90","author":"Diawara M","year":"2003","journal-title":"Parasitol Res"},{"issue":"3","key":"ref10","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1590\/S1984-29612014054","article-title":"Autochthonous infection of buffaloes and cattle by Fasciola hepatica in Minas Gerais, Brazil","volume":"23","author":"Dracz RM","year":"2014","journal-title":"Rev Bras Parasitol Vet"},{"issue":"3","key":"ref11","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1051\/parasite\/2012193271","article-title":"Local adaptation of the trematode Fasciola hepatica to the snail Galba truncatula.","volume":"19","author":"Dreyfuss G","year":"2012","journal-title":"Parasite"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.molbiopara.2008.05.002","article-title":"Excretory\u2013secretory products of larval Fasciola hepatica investigated using a two-dimensional proteomic approach","volume":"161","author":"Gourbal BE","year":"2008","journal-title":"Mol Biochem Parasitol"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1017\/S0022149X10000350","article-title":"First report of larval stages of Fasciola hepatica in a wild population of Pseudosuccinea columella from Cuba and the Caribbean","volume":"85","author":"Guti\u00e9rrez A","year":"2011","journal-title":"J Helminthol"},{"issue":"4","key":"ref14","first-page":"647","article-title":"Effect of Fasciola hepatica larvae on the ultrastructure of digestivegland of Galba truncatula.","volume":"47","author":"Humiczewska M","year":"2000","journal-title":"Wiad Parazytol"},{"issue":"2","key":"ref15","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1093\/icb\/43.2.305","article-title":"Cellular receptors and signal transduction in molluscan hemocytes: connections with the innate immune system of vertebrates","volume":"43","author":"Humphries JE","year":"2003","journal-title":"Integr Comp Biol"},{"issue":"2","key":"ref16","doi-asserted-by":"crossref","first-page":"27","DOI":"10.4322\/rbpv.01802006","article-title":"Occurrence of Fasciola hepatica (Linnaeus, 1758) infection in Brazilian cattle of Minas Gerais, Brazil","volume":"18","author":"Lima WS","year":"2009","journal-title":"Rev Bras Parasitol Vet"},{"issue":"3","key":"ref17","doi-asserted-by":"crossref","first-page":"503","DOI":"10.2307\/3282128","article-title":"Alterations in Biomphalaria glabrata plasma induced by infection with the digenetic trematode Echinostoma paraensei.","volume":"73","author":"Loker ES","year":"1987","journal-title":"J Parasitol"},{"issue":"3","key":"ref18","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1590\/S0074-02762001000300019","article-title":"Anesthesia of Biomphalaria spp. (Mollusca, Gastropoda): sodium pentobarbital is the drug of choice","volume":"96","author":"Martins-Sousa RL","year":"2001","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"6","key":"ref19","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1007\/s00436-003-0985-4","article-title":"Silica treatment increases the susceptibility of the Cabo Frio strain of Biomphalaria tenagophila to Schistosoma mansoni infection but does not alter the natural resistance of the Taim strain","volume":"91","author":"Martins-Souza RL","year":"2003","journal-title":"Parasitol Res"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1017\/S0022149X07873585","article-title":"Development of Fasciola hepatica in Lymnaea columella infected with miracidia derived from cattle and marmoset infections","volume":"82","author":"Mendes EA","year":"2008","journal-title":"J Helminthol"},{"issue":"1","key":"ref21","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1590\/1808-1657v69n1p0292002","article-title":"Ocorr\u00eancia de Lymnaea columella Say, 1817 infectada naturalmente por Fasciola hepatica (Linnaeus, 1758), no vale do Ribeira, S\u00e3o Paulo, Brasil","volume":"69","author":"Oliveira S","year":"2002","journal-title":"Arq Inst Biol (Sao Paulo)"},{"issue":"11-12","key":"ref22","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1111\/j.1365-3024.2008.01062.x","article-title":"Participation of cell\u2010free haemolymph of Biomphalaria tenagophila in the defence mechanism against Schistosoma mansoni sporocysts","volume":"30","author":"Pereira CAJ","year":"2008","journal-title":"Parasite Immunol"},{"issue":"3","key":"ref23","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1017\/S0022149X09222528","article-title":"Redial generations of Fasciola hepatica: a review","volume":"83","author":"Rondelaud D","year":"2009","journal-title":"J Helminthol"},{"issue":"1","key":"ref24","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1590\/S0034-89101980000100004","article-title":"Infec\u00e7\u00e3o experimental de Lymnaea columella por Fasciola hepatica.","volume":"14","author":"Ueta MT","year":"1980","journal-title":"Rev Saude Publica"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1590\/S0034-89101980000200010","article-title":"Ocorr\u00eancia de infec\u00e7\u00e3o natural de Fasciola hepatica Linnaeus, 1758 em Lymnaea columella Say, 1817 no Vale do Para\u00edba, SP, Brasil","volume":"14","author":"Ueta MT","year":"1980","journal-title":"Rev Saude Publica"},{"issue":"1","key":"ref26","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/BF00536337","article-title":"Alterations in the internal defence system of the pond snail Lymnaea stagnalis induced by infection with the schistosome Trichobilharzia ocellata.","volume":"73","author":"Van der Knaap WPW","year":"1987","journal-title":"Parasitol Res"},{"issue":"3-4","key":"ref27","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.prevetmed.2012.10.017","article-title":"Conceptual framework for analysing farm-specific economic effects of helminth infections in ruminants and control strategies","volume":"109","author":"Van der Voort M","year":"2013","journal-title":"Prev Vet Med"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1051\/parasite\/2015015","article-title":"Fasciola hepatica: comparative metacercarial productions in experimentally-infected Galba truncatula and Pseudosuccinea columella.","volume":"22","author":"Vignoles P","year":"2015","journal-title":"Parasite"},{"issue":"7","key":"ref29","doi-asserted-by":"crossref","first-page":"S143","DOI":"10.1017\/S0031182001007685","article-title":"Receptor-ligand interactions and cellular signalling at the host\u2013parasite interface","volume":"123","author":"Yoshino TP","year":"2001","journal-title":"Parasitology"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rbpv\/v26n4\/1984-2961-rbpv-S1984-29612017057.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T23:03:34Z","timestamp":1750979014000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612017000400411&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,9]]},"references-count":29,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["S1984-29612017000400411"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612017057","relation":{},"ISSN":["1984-2961","0103-846X"],"issn-type":[{"value":"1984-2961","type":"electronic"},{"value":"0103-846X","type":"print"}],"subject":[],"published":{"date-parts":[[2017,11,9]]}}}