{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T09:36:46Z","timestamp":1781948206329,"version":"3.54.5"},"reference-count":45,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract Rearing free-range chicken is based on grazing feeding patterns, and these animals could be potential environmental contaminants of Cryptosporidium oocysts for humans and other animals. Therefore, the present study aimed to evaluate the molecular prevalence of Cryptosporidium spp. in free-range chickens from Brazil. A total of 351 fecal samples from chickens were examined from 20 farms. For detection of Cryptosporidium spp., 18S rRNA gene fragments were amplified using a nested PCR reaction. Positive samples were sent for sequencing. The overall prevalence of Cryptosporidium was 25.6% (95% CI = 21.2% - 30.6%). Sequencing of the amplified fragments allowed for the identification of three species: C. meleagridis in 57 (62.6%), C. baileyi in 15 (16.4%), C. parvum in 3 (3.2%) samples, and a new Cryptosporidium genotype (C. genotype BrPR1) in 3 (3.2%) samples. Cryptosporidium genotype BrPR1 has not yet been classified as a species, and its host spectrum is not known. Cryptosporidium, including zoonotic species, exists at a high prevalence in free-range chickens within the region studied.<\/jats:p>","DOI":"10.1590\/s1984-29612017068","type":"journal-article","created":{"date-parts":[[2017,11,25]],"date-time":"2017-11-25T22:30:22Z","timestamp":1511649022000},"page":"472-478","source":"Crossref","is-referenced-by-count":12,"title":["The first study of molecular prevalence and species characterization of Cryptosporidium in free-range chicken (Gallus gallus domesticus) from Brazil"],"prefix":"10.1590","volume":"26","author":[{"given":"Maria Paula de Carvalho","family":"Ewald","sequence":"first","affiliation":[{"name":"Universidade Estadual de Londrina, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felippe Danyel Cardoso","family":"Martins","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eloiza Teles","family":"Caldart","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando Emmanuel Gon\u00e7alves","family":"Vieira","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Norte do Paran\u00e1, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milton Hissashi","family":"Yamamura","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Pedro","family":"Sasse","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Daniel de","family":"Barros","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta Lemos","family":"Freire","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Italmar Teodorico","family":"Navarro","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Luis","family":"Garcia","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2017,11,27]]},"reference":[{"issue":"3-4","key":"ref1","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.vetpar.2013.02.022","article-title":"Common occurrence of zoonotic pathogen Cryptosporidium meleagridis in broiler chickens and turkeys in Algeria","volume":"196","author":"Baroudi D","year":"2013","journal-title":"Vet Parasitol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1111\/j.1550-7408.2003.tb00620.x","article-title":"Cryptosporidium species and genotypes in HIV-positive patients in Lima, Peru","volume":"50","author":"Cama VA","year":"2003","journal-title":"J Eukaryot Microbiol"},{"issue":"5","key":"ref3","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1086\/519842","article-title":"Differences in clinical manifestations among Cryptosporidium species and subtypes in HIV-infected persons","volume":"196","author":"Cama VA","year":"2007","journal-title":"J Infect Dis"},{"issue":"3","key":"ref4","first-page":"119","article-title":"Transmiss\u00e3o experimental de Cryptosporidium baileyi (Apicomplexa: Cryptosporidiidae) isolado de frango de corte \u00e0 codorna japonesa (Coturnix japonica)","volume":"14","author":"Cardozo SV","year":"2005","journal-title":"Rev Bras Parasitol Vet"},{"issue":"2","key":"ref5","doi-asserted-by":"crossref","first-page":"238","DOI":"10.4269\/ajtmh.2011.10-0664","article-title":"Cryptosporidium meleagridis: infectivity in healthy adult volunteers","volume":"85","author":"Chappell CL","year":"2011","journal-title":"Am J Trop Med Hyg"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"50","DOI":"10.4269\/ajtmh.14-0525","article-title":"Cryptosporidium muris: infectivity and illness in healthy adult volunteers","volume":"92","author":"Chappell CL","year":"2015","journal-title":"Am J Trop Med Hyg"},{"issue":"2","key":"ref7","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.1550-7408.1986.tb05608.x","article-title":"The life cycle of Cryptosporidium baileyi n. sp. 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