{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:30:01Z","timestamp":1762522201824},"reference-count":48,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract  We evaluated the distribution of piroplasmids in equids from the Mato Grosso state in Midwestern Brazil using molecular methods and the interspecific genetic diversity. For this, 1,624 blood samples of equids from 973 farms were examined by PCR, using primer pairs that amplify a fragment of the genes rap-1 and ema-1 of Babesia caballi and Theileria equi, respectively. For molecular characterization and phylogenetic studies, 13 and 60 sequences of the rap-1 and ema-1 genes, respectively, were used to build a dendogram using maximum parsimony. B. caballi and T. equi were detected in 4.11% and 28.16% of the farms, respectively, and molecular prevalence was 2.74% for B. caballi and 25.91% for T. equi. The location of the farms and animals raised in the Pantanal ecoregion influence the probability of equids testing positive for B. caballi and T. equi . Moreover, age and herd purpose were variables significantly associated with T . equi infection. The sequences of B. caballi presented 1.95% intraspecific variability, contrasting with 2.99% in T. equi. Dendrograms for both species demonstrated the presence of subgroups with high values of support of branches. However, it is not possible to associate these groups with geographic origin and\/or ecoregion.<\/jats:p>","DOI":"10.1590\/s1984-296120180048","type":"journal-article","created":{"date-parts":[[2018,8,31]],"date-time":"2018-08-31T13:47:50Z","timestamp":1535723270000},"page":"464-472","source":"Crossref","is-referenced-by-count":7,"title":["Molecular survey and genetic diversity of piroplasmids in equids from Midwestern Brazil"],"prefix":"10.1590","volume":"27","author":[{"given":"Fabio Bernardo","family":"Schein","sequence":"first","affiliation":[{"name":"Universidade Federal de Mato Grosso,  Brasil; Universidade de Cuiab\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maerle Oliveira","family":"Maia","sequence":"additional","affiliation":[{"name":"Universidade Federal de Mato Grosso,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rute","family":"Witter","sequence":"additional","affiliation":[{"name":"Universidade Federal de Mato Grosso,  Brasil; Instituto Federal de Rond\u00f4nia,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arlei","family":"Marcili","sequence":"additional","affiliation":[{"name":"Universidade de Santo Amaro,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00e1zaro Manoel de","family":"Camargo","sequence":"additional","affiliation":[{"name":"Universidade de Cuiab\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Val\u00e9ria","family":"Dutra","sequence":"additional","affiliation":[{"name":"Universidade Federal de Mato Grosso,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciano","family":"Nakazato","sequence":"additional","affiliation":[{"name":"Universidade Federal de Mato Grosso,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefhano Lu\u00eds","family":"Candido","sequence":"additional","affiliation":[{"name":"Universidade Federal de Mato Grosso,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elianara Martins de","family":"Almeida","sequence":"additional","affiliation":[{"name":"Universidade Federal de Mato Grosso,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anderson Castro Soares de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Federal de Mato Grosso,  Brasil; Universidade Federal de Mato Grosso,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard de Campos","family":"Pacheco","sequence":"additional","affiliation":[{"name":"Universidade Federal de Mato Grosso,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2018,8,30]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1111\/j.1865-1682.2011.01244.x","article-title":"Equine Babesiosis: seroprevalence, risk factors and comparison of different diagnostic methods in Jordan","volume":"59","author":"Abutarbush SM","year":"2012","journal-title":"Transbound Emerg Dis"},{"issue":"1-2","key":"ref2","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.vetpar.2004.12.018","article-title":"Development of a single-round and multiplex PCR method for the simultaneous detection of Babesia caballi and Babesia equi in horse blood","volume":"129","author":"Alhassan A","year":"2005","journal-title":"Vet Parasitol"},{"issue":"4","key":"ref3","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1590\/S1519-69842008000500005","article-title":"Biodiversity of the Pantanal: response to seasonal flooding regime and to environmental degradation","volume":"68","author":"Alho CJR","year":"2008","journal-title":"Braz J Biol"},{"issue":"4","key":"ref4","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1590\/S1984-29612010000400007","article-title":"Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, S\u00e3o Paulo State, Brazil","volume":"19","author":"Baldani CD","year":"2010","journal-title":"Rev Bras Parasitol Vet"},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1590\/1678-4162-7930","article-title":"Detec\u00e7\u00e3o de Theileria equi e Babesia caballi e anticorpos anti-Ehrlichia spp. em equ\u00eddeos do Pantanal Mato-Grossense, Brasil","volume":"67","author":"Barros EM","year":"2015","journal-title":"Arq Bras Med Vet Zootec"},{"key":"ref6","series-title":"Preval\u00eancia, distribui\u00e7\u00e3o espacial de focos e poss\u00edveis fatores de risco associados \u00e0 anemia infecciosa equina (aie) no estado de Mato Grosso","author":"Barros ML","year":"2017"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/j.ttbdis.2016.01.011","article-title":"Babesia caballi and Theileria equi infections in horses in Central-Southern Italy: Sero-molecular survey and associated risk factors","volume":"7","author":"Bartolom\u00e9 Del Pino LE","year":"2016","journal-title":"Ticks Tick Borne Dis"},{"issue":"9","key":"ref8","doi-asserted-by":"crossref","first-page":"4172","DOI":"10.1128\/JCM.41.9.4172-4177.2003","article-title":"Development and evaluation of a seminested PCR for detection and differentiation of Babesia gibsoni (Asian genotype) and B. canis DNA in canine blood samples","volume":"41","author":"Birkenheuer AJ","year":"2003","journal-title":"J Clin Microbiol"},{"issue":"3","key":"ref9","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1590\/s1984-29612017046","article-title":"Genetic diversity of piroplasmids species in equids from island of S\u00e3o Lu\u00eds, northeastern Brazil","volume":"26","author":"Braga MDSCO","year":"2017","journal-title":"Rev Bras Parasitol Vet"},{"key":"ref10","series-title":"Mapa dos Biomas Mato-Grossenses","year":"2014"},{"issue":"5","key":"ref11","doi-asserted-by":"crossref","first-page":"777","DOI":"10.2307\/3280361","article-title":"Development, survival, and oviposition of the rabbit tick, Haemaphysalis leporispalustris (Packard) (Acari: Ixodidae), at constant temperatures","volume":"65","author":"Campbell A","year":"1979","journal-title":"J Parasitol"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/0007-1935(92)90061-5","article-title":"Equine piroplasmosis: a review","volume":"148","author":"de Waal DT","year":"1992","journal-title":"Br Vet J"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.cimid.2016.06.004","article-title":"Serological and molecular detection of Theileria equi in sport horses of northeastern Brazil","volume":"47","author":"Ferreira EP","year":"2016","journal-title":"Comp Immunol Microbiol Infect Dis"},{"key":"ref14","first-page":"18","article-title":"Prevalence and risk factors of Theileria equi infection in horses in Minas Gerais, Brazil","volume":"3-4","author":"Guimar\u00e3es AM","year":"2016","journal-title":"Vet Parasitol"},{"issue":"1","key":"ref15","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1515\/ap-2017-0025","article-title":"Prevalence and molecular characterization of Theileria equi and Babesia caballi in jereed horses in Erzurum, Turkey","volume":"62","author":"Guven E","year":"2017","journal-title":"Acta Parasitol"},{"issue":"1","key":"ref16","first-page":"86","article-title":"Microscopic and molecular detection of Theileria (Babesia ) equi infection in equids of Kurdistan province, Iran","volume":"11","author":"Habibi G","year":"2016","journal-title":"Iran J Parasitol"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/s00436-007-0726-1","article-title":"Detection and molecular characterization of Babesia caballi and Theileria equi isolates from endemic areas of Brazil","volume":"102","author":"Heim A","year":"2007","journal-title":"Parasitol Res"},{"key":"ref18","series-title":"Relat\u00f3rio de equinos existentes","year":"2014"},{"issue":"2","key":"ref19","first-page":"123","article-title":"Control of equine piroplasmosis in Brazil","volume":"66","author":"Kerber CE","year":"1999","journal-title":"Onderstepoort J Vet Res"},{"issue":"4","key":"ref20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4322\/rbpv.01804001","article-title":"Prevalence of equine piroplasmosis and its association with tick infestation in the State of S\u00e3o Paulo, Brazil","volume":"18","author":"Kerber CE","year":"2009","journal-title":"Rev Bras Parasitol Vet"},{"key":"ref21","series-title":"Analysis of health surveys","author":"Korn EL","year":"1999"},{"issue":"3","key":"ref22","first-page":"115","article-title":"Efeito da imers\u00e3o de f\u00eameas ingurgitadas de Boophilus microplus (Canestrini, 1887) (Acari: Ixodidae) em \u00e1gua destilada sobre os par\u00e2metros biol\u00f3gicos ligados \u00e0 oviposi\u00e7\u00e3o","volume":"12","author":"Louzada GL","year":"2003","journal-title":"Rev Bras Parasitol Vet"},{"issue":"3-4","key":"ref23","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.vetpar.2011.11.069","article-title":"Molecular and serological detection of Theileria equi and Babesia caballi in donkeys (Equus asinus) in Brazil","volume":"186","author":"Machado RZ","year":"2012","journal-title":"Vet Parasitol"},{"issue":"6","key":"ref24","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1007\/s004360050431","article-title":"Redescription of Babesia equi Laveran, 1901 as Theileria equi Mehlhorn, Schein, 1998","volume":"84","author":"Mehlhorn H","year":"1998","journal-title":"Parasitol Res"},{"key":"ref25","first-page":"14","article-title":"GeneDoc: analysis and visualization of genetic variation","volume":"4","author":"Nicholas KB","year":"1997","journal-title":"Embnew News"},{"issue":"1","key":"ref26","first-page":"13","article-title":"Efeito da imers\u00e3o de larvas n\u00e3o alimentadas de Anocentor nitens (Neumann, 1897) e de Amblyomma cajennense (Fabricius, 1787) (Acari: Ixodidae) em \u00e1gua destilada","volume":"13","author":"Paula AR","year":"2004","journal-title":"Rev Bras Parasitol Vet"},{"issue":"5","key":"ref27","doi-asserted-by":"crossref","first-page":"2017","DOI":"10.1007\/s00436-013-3360-0","article-title":"Molecular epidemiology of Theileria equi in horses and their association with possible tick vectors in the state of Rio de Janeiro, Brazil","volume":"112","author":"Peckle M","year":"2013","journal-title":"Parasitol Res"},{"issue":"2","key":"ref28","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.ttbdis.2017.11.011","article-title":"Molecular characterization of Theileria equi in horses from the state of Rio de Janeiro, Brazil","volume":"9","author":"Peckle M","year":"2018","journal-title":"Ticks Tick Borne Dis"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2015\/236278","article-title":"Detection of Babesia caballi and Theileria equi in blood from equines from four indigenous communities in Costa Rica","volume":"2015","author":"Posada-Guzm\u00e1n MF","year":"2015","journal-title":"Vet Med Int"},{"issue":"1","key":"ref30","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1590\/S1984-29612014012","article-title":"Seroprevalence rates of antibodies against Theileria equi in team roping horses from central-western region of Paran\u00e1","volume":"23","author":"Prochno HC","year":"2014","journal-title":"Rev Bras Parasitol Vet"},{"key":"ref31","series-title":"R: A language and environment for statistical computing","year":"2015"},{"issue":"2","key":"ref32","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/s10493-015-9991-3","article-title":"Nellore cattle (Bos indicus) and ticks within the Brazilian Pantanal: ecological relationships","volume":"68","author":"Ramos VN","year":"2016","journal-title":"Exp Appl Acarol"},{"issue":"7","key":"ref33","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.jevs.2013.03.189","article-title":"Equine piroplasmosis","volume":"33","author":"Rothschild CM","year":"2013","journal-title":"J Equine Vet Sci"},{"issue":"05","key":"ref34","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1017\/S0031182008004204","article-title":"Estimation of the transmission dynamics of Theileria equi and Babesia caballi in horses","volume":"135","author":"R\u00fcegg SR","year":"2008","journal-title":"Parasitology"},{"key":"ref35","series-title":"Molecular cloning: a laboratory manual.","author":"Sambrook JJ","year":"2001"},{"issue":"3","key":"ref36","first-page":"1127","article-title":"Efeito da imers\u00e3o em \u00e1gua destilada sobre os par\u00e2metros reprodutivos de tele\u00f3ginas de Rhipicephalus (Boophilus ) microplus.","volume":"33","author":"Santos FCC","year":"2012","journal-title":"Semina: Ci\u00eanc Agr\u00e1r"},{"issue":"3","key":"ref37","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1590\/S1984-29612011000300011","article-title":"Factors associated to Theileria equi in equids of two microregions from Rio de Janeiro, Brazil","volume":"20","author":"Santos TM","year":"2011","journal-title":"Rev Bras Parasitol Vet"},{"key":"ref38","series-title":"Elementary survey sampling","author":"Schaeffer RL","year":"2011"},{"issue":"1","key":"ref39","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1146\/annurev-ento-010814-021110","article-title":"Vector ecology of equine piroplasmosis","volume":"60","author":"Scoles GA","year":"2015","journal-title":"Annu Rev Entomol"},{"key":"ref40","series-title":"PAUP: Phylogenetic analysis using parsimony. Beta Version 4.0b10.","author":"Swofford DL","year":"2002"},{"issue":"24","key":"ref41","doi-asserted-by":"crossref","first-page":"4876","DOI":"10.1093\/nar\/25.24.4876","article-title":"The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools","volume":"25","author":"Thompson JD","year":"1997","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"ref42","first-page":"70","article-title":"Aspectos epidemiol\u00f3gicos da theileriose equina e sua rela\u00e7\u00e3o com o carrapato Rhipicephalus (Boophilus) microplus em duas propriedades na regi\u00e3o da campanha do Rio Grande do Sul - Brasil","volume":"71","author":"Torres AJ","year":"2012","journal-title":"Rev Ibero-Latinoam Parasitol"},{"issue":"1-2","key":"ref43","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.vetpar.2006.01.035","article-title":"Babesia: a historical overview","volume":"138","author":"Uilenberg G","year":"2006","journal-title":"Vet Parasitol"},{"issue":"2","key":"ref44","doi-asserted-by":"crossref","first-page":"227","DOI":"10.4172\/2161-1165.1000227","article-title":"Multicollinearity in Regression Analyses Conducted In Epidemiologic Studies","volume":"6","author":"Vatcheva KP","year":"2016","journal-title":"Epidemiology (Sunnyvale)"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.actatropica.2017.12.028","article-title":"Serological detection and molecular characterization of piroplasmids in equids in Brazil","volume":"179","author":"Vieira MIB","year":"2018","journal-title":"Acta Trop"},{"issue":"6","key":"ref46","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.ttbdis.2013.07.005","article-title":"Seroepidemiological survey of Theileria equi and Babesia caballi in horses from a rural and from urban areas of Paran\u00e1 State, southern Brazil","volume":"4","author":"Vieira TSWJ","year":"2013","journal-title":"Ticks Tick Borne Dis"},{"issue":"6","key":"ref47","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1111\/jvim.12168","article-title":"Review of equine piroplasmosis","volume":"27","author":"Wise LN","year":"2013","journal-title":"J Vet Intern Med"},{"issue":"3","key":"ref48","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1016\/j.cveq.2014.08.008","article-title":"Equine piroplasmosis","volume":"30","author":"Wise LN","year":"2014","journal-title":"Vet Clin North Am Equine Pract"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rbpv\/v27n4\/1984-2961-rbpv-S1984-296120180048.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,12,21]],"date-time":"2018-12-21T18:24:34Z","timestamp":1545416674000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612018000400464&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,30]]},"references-count":48,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2018,12]]}},"alternative-id":["S1984-29612018000400464"],"URL":"https:\/\/doi.org\/10.1590\/s1984-296120180048","relation":{},"ISSN":["1984-2961","0103-846X"],"issn-type":[{"value":"1984-2961","type":"electronic"},{"value":"0103-846X","type":"print"}],"subject":[],"published":{"date-parts":[[2018,8,30]]}}}