{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T23:36:07Z","timestamp":1781307367528,"version":"3.54.1"},"reference-count":52,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract Visceral leishmaniasis (VL) is a disease caused by the protozoa Leishmania infantum and can cause an inflammatory reaction in the gastrointestinal tract, however the role of granulocytic cells (neutrophils, eosinophils, and mast cells) in the intestine of dogs infected is not fully understood. We performed a quantitative analysis these cells in the intestinal wall of dogs with canine visceral leishmaniasis (CVL). Twenty dogs were assigned to one of three groups: group 1 (G1, n=8), dogs with CVL and L. infantum amastigotes in the intestine; group 2 (G2, n=9), dogs with CVL but without intestinal amastigotes; and group 3 (G3, n=3), uninfected dogs (control group). Granulocytic cells were counted in the crypt-villus unit (mucosa), submucosa, and muscle layer of the intestinal mucosa. Cell counts were higher in the intestinal wall of dogs from G2 followed by G1 and G3 (p\u22640.05). In G1, there was a low inverse correlation between parasite burden of the small intestine and granulocyte counts (r= -0.1, p\u22640.01). However, in G2 dogs, mast cell and eosinophil numbers showed positive correlation (r=0.85, p\u22640.01). The granulocytic cell hyperplasia observed in the intestine of L. infantum-infected dogs suggests that these cells may be involved in the cell-mediated immune response for parasite elimination.<\/jats:p>","DOI":"10.1590\/s1984-296120180085","type":"journal-article","created":{"date-parts":[[2018,12,4]],"date-time":"2018-12-04T17:12:16Z","timestamp":1543943536000},"page":"430-438","source":"Crossref","is-referenced-by-count":5,"title":["Neutrophils, eosinophils, and mast cells in the intestinal wall of dogs naturally infected with Leishmania infantum"],"prefix":"10.1590","volume":"27","author":[{"given":"Diogo Tiago da","family":"Silva","sequence":"first","affiliation":[{"name":"Universidade Estadual Paulista, Brasil; Universidade de S\u00e3o Paulo, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Luana","family":"Alves","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista, Brasil; Universidade de S\u00e3o Paulo, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00falio Cesar Pereira","family":"Spada","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista, Brasil; Universidade de S\u00e3o Paulo, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita de C\u00e1ssia Viveiros da","family":"Silveira","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tr\u00edcia Maria Ferreira de Sousa","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo, Brasil; Universidade de S\u00e3o Paulo, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilma Aparecida","family":"Starke-Buzetti","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2018,12]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"281","DOI":"10.4049\/jimmunol.147.1.281","article-title":"Soluble IL-2 receptor as an agent of serum mediated suppression in human visceral leishmaniasis","volume":"147","author":"Barral-Netto M","year":"1991","journal-title":"J Immunol"},{"issue":"2","key":"ref2","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1053\/tvjl.2000.0556","article-title":"Atypical Forms of Canine Leishmaniosis","volume":"162","author":"Blavier A","year":"2001","journal-title":"Vet J"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.vetpar.2017.05.028","article-title":"Association between mast cells, tissue remodelation and parasite burden in the skin of dogs with visceral leishmaniasis","volume":"243","author":"Cardoso JMO","year":"2017","journal-title":"Vet Parasitol"},{"key":"ref4","series-title":"Current Veterinary Therapy: IX Small Animal Practice.","first-page":"896","volume-title":"Diagnosis and management of chronic colitis in the dogs and cat.","author":"Chiapella A","year":"1986"},{"issue":"21","key":"ref5","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1136\/vr.141.21.539","article-title":"A retrospective clinical study of canine leishmaniasis in 150 dogs naturally infected by Leishmania infantum.","volume":"141","author":"Ciaramella P","year":"1997","journal-title":"Vet Rec"},{"issue":"2","key":"ref6","first-page":"369","article-title":"Leucograma e metabolismo oxidativo dos neutr\u00f3filos de c\u00e3es com leishmaniose visceral antes e ap\u00f3s o tratamento com antimoniato de meglumina e alopurinol","volume":"11","author":"Ciarlini PC","year":"2010","journal-title":"Cienc Anim Bras"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/S0035-9203(03)00002-6","article-title":"PCR as a rapid and sensitive tool in the diagnosis of human and canine leishmaniasis using Leishmania donovani s.l. - 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