{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T01:13:08Z","timestamp":1771636388019,"version":"3.50.1"},"reference-count":27,"publisher":"FapUNIFESP (SciELO)","issue":"1","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract  Currently, albendazole is one of the most commonly used drugs because of its affordability. The objective was to evaluate the histopathology of Fasciola hepatica specimens. For this, the efficacy test was performed on sheep treated with albendazole at the dose recommended for F. hepatica, in which the helminths recovered at necropsy were counted and separated for histology. Spermatogenic cells from parasites recovered from treated and control sheep were examined by microscopy. The fecal egg-count reduction test revealed 97.06% efficacy of albendazole in the treatment of F. hepatica. Changes in testicular tubule cells started 48 hours after treatment and became evident within 72 hours, at which point it became difficult to identify cell types. Primary and secondary spermatogonia became increasingly rare and intercellular vacuolization was more evident. Signs of apoptosis, with pycnotic nuclei and evidence of keriorrexia were observed at all times. Cell debris was identified 96 hours after treatment. The results indicated that parasitic spermatogenesis was severely affected by albendazole and demonstrated the importance of the use of histopathology for the diagnosis of therapeutic efficacy in field strains.<\/jats:p>","DOI":"10.1590\/s1984-296120180095","type":"journal-article","created":{"date-parts":[[2019,2,15]],"date-time":"2019-02-15T18:56:30Z","timestamp":1550256990000},"page":"33-39","source":"Crossref","is-referenced-by-count":1,"title":["Microscopic alterations in Fasciola hepatica from sheep treated with albendazole"],"prefix":"10.1590","volume":"28","author":[{"given":"Milena Batista","family":"Carneiro","sequence":"first","affiliation":[{"name":"Universidade Federal Rural do Rio de Janeiro,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara Rauta","family":"Avelar","sequence":"additional","affiliation":[{"name":"Universidade Federal do Esp\u00edrito Santo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anderson Barros","family":"Archanjo","sequence":"additional","affiliation":[{"name":"Universidade Federal do Esp\u00edrito Santo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8700-3065","authenticated-orcid":false,"given":"Isabella Vilhena Freire","family":"Martins","sequence":"additional","affiliation":[{"name":"Universidade Federal do Esp\u00edrito Santo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louisiane de Carvalho","family":"Nunes","sequence":"additional","affiliation":[{"name":"Universidade Federal do Esp\u00edrito Santo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio Barbour","family":"Scott","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural do Rio de Janeiro,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2019,3]]},"reference":[{"issue":"2-4","key":"ref1","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.vetpar.2009.05.014","article-title":"Comparative assessment of albendazole and triclabendazole ovicidal activity on Fasciola hepatica eggs","volume":"164","author":"Alvarez L","year":"2009","journal-title":"Vet Parasitol"},{"issue":"16","key":"ref2","first-page":"323","article-title":"Atualiza\u00e7\u00f5es no controle parasit\u00e1rio da fasciol\u00edase em bovinos","volume":"9","author":"Alves DP","year":"2013","journal-title":"Enc Biosfera"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1017\/S0031182002002664","article-title":"Fasciola hepatica: surface and internal tegumental changes induced by treatment in vitro with the sulphoxide metabolite of albendazole (\u2018Valbazen\u2019)","volume":"126","author":"Buchanan JF","year":"2003","journal-title":"Parasitology"},{"issue":"1-2","key":"ref4","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/0304-4017(92)90141-U","article-title":"World Association for the Advancement of Veterinary Parasitology (W. 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