{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T06:31:24Z","timestamp":1781591484817,"version":"3.54.5"},"reference-count":19,"publisher":"FapUNIFESP (SciELO)","issue":"3","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract A juvenile subantarctic fur seal (Arctocephalus tropicalis) found dead in Santa Catarina state, southern Brazil, presented with disseminated verminous pneumonia due to Parafilaroides sp. A concomitant infection with two different gammaherpesviruses was identified by PCR in different tissues; one of them possibly a novel species (tentatively named Otariid herpesvirus 7). Sarcocystis sp. DNA was identified molecularly in skeletal muscle samples with intrasarcoplasmic bradyzoites and no apparent tissue response. All analyzed samples (mandibular, laryngeal, tracheal, and mesenteric lymph nodes, and lung) were PCR-negative for Brucella spp. The most likely cause of death was severe pulmonary parafilaroidiasis. The pathogenic role of the gammaherpesviruses in several of the tissues was not evident. This study describes the pathogenicity of Parafilaroides sp. in a subantarctic fur seal, widens the host range of herpesvirus in pinnipeds, and reports the first molecular identification of Sarcocystis sp. in this species.<\/jats:p>","DOI":"10.1590\/s1984-29612019029","type":"journal-article","created":{"date-parts":[[2019,7,27]],"date-time":"2019-07-27T15:27:49Z","timestamp":1564241269000},"page":"499-503","source":"Crossref","is-referenced-by-count":13,"title":["Fatal pulmonary parafilaroidiasis in a free-ranging subantarctic fur seal (Arctocephalus tropicalis) coinfected with two gammaherpesviruses and Sarcocystis sp."],"prefix":"10.1590","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1450-400X","authenticated-orcid":false,"given":"Laura","family":"Reisfeld","sequence":"first","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil; Aqu\u00e1rio de S\u00e3o Paulo,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Sacrist\u00e1n","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang\u00e9lica Mar\u00eda","family":"S\u00e1nchez-Sarmiento","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samira","family":"Costa-Silva","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josu\u00e9","family":"D\u00edaz-Delgado","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K\u00e1tia Regina","family":"Groch","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"Marigo","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Carolina","family":"Ewbank","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00edntia Maria","family":"Favero","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Mariotti","family":"Guerra","sequence":"additional","affiliation":[{"name":"Instituto Adolfo Lutz,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Albergaria","family":"R\u00e9ssio","sequence":"additional","affiliation":[{"name":"Instituto Adolfo Lutz,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta Jussara","family":"Cremer","sequence":"additional","affiliation":[{"name":"Universidade da Regi\u00e3o de Joinville,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Esper\u00f3n","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal,  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Luiz","family":"Cat\u00e3o-Dias","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2019,7]]},"reference":[{"issue":"4","key":"ref1","first-page":"271","article-title":"Detection of Brucella melitensis and Brucella abortus by DNA amplification","volume":"95","author":"Baily GG","year":"1992","journal-title":"J Trop Med Hyg"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"88","DOI":"10.7589\/2015-03-060","article-title":"A novel gammaherpesvirus in northern fur seals (Callorhinus ursinus) is closely related to the California sea lion (Zalophus californianus) carcinoma associated Otarine herpesvirus-1","volume":"52","author":"Cort\u00e9s-Hinojosa G","year":"2016","journal-title":"J Wildl Dis"},{"key":"ref3","series-title":"CRC Handbook of marine mammal medicine","first-page":"357","volume-title":"Parasitic diseases.","author":"Dailey MD","year":"2001","edition":"2nd ed."},{"issue":"1","key":"ref4","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1645\/GE-1521.1","article-title":"A new species of Parafilaroides (Nematoda: Filaroididae) in three species of fur seals (Carnivora: Otariidae) from the southern hemisphere","volume":"95","author":"Dailey MD","year":"2009","journal-title":"J Parasitol"},{"key":"ref5","series-title":"Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses.","first-page":"193","volume-title":"Family Herpesviridae.","author":"Davison A","year":"2005"},{"issue":"7","key":"ref6","doi-asserted-by":"crossref","first-page":"3509","DOI":"10.1128\/JVI.02646-07","article-title":"Novel mammalian herpesviruses and lineages within the Gammaherpesvirinae: Cospeciation and interspecies transfer","volume":"82","author":"Ehlers B","year":"2008","journal-title":"J Virol"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1177\/104063879700900311","article-title":"Evidence of Brucella infection in Parafilaroides lungworms in a Pacific harbor seal (Phoca vitulina richardsi)","volume":"9","author":"Garner MM","year":"1997","journal-title":"J Vet Diagn Invest"},{"key":"ref8","series-title":"Marine mammals ashore: a field guide for strandings.","author":"Geraci JR","year":"2005","edition":"2nd"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"22766","DOI":"10.3402\/jom.v5i0.22766","article-title":"Herpesviruses: latency and reactivation\u2013viral strategies and host response","volume":"5","author":"Grinde B","year":"2013","journal-title":"J Oral Microbiol"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1638\/2015-0090.1","article-title":"Identification of respiratory and gastrointestinal parasites of three species of pinnipeds (Arctocephalus australis, Arctocephalus gazella, and Otaria flavescens) in southern Brazil","volume":"47","author":"Jacobus K","year":"2016","journal-title":"J Zoo Wildl Med"},{"issue":"7","key":"ref11","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar S","year":"2016","journal-title":"Mol Biol Evol"},{"issue":"6","key":"ref12","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1354\/vp.37-6-609","article-title":"Common metastatic carcinoma of California sea lions (Zalophus californianus): evidence of genital origin and association with novel gammaherpesvirus","volume":"37","author":"Lipscomb TP","year":"2000","journal-title":"Vet Pathol"},{"key":"ref13","series-title":"Parasitic diseases of wild mammals. 2nd.","first-page":"279","volume-title":"Lungworms of marine mammals.","author":"Measures LN","year":"2001"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1186\/s12917-014-0308-2","article-title":"First molecular detection and characterization of herpesvirus and poxvirus in a Pacific walrus (Odobenus rosmarus divergens)","volume":"10","author":"Melero M","year":"2014","journal-title":"BMC Vet Res"},{"issue":"3","key":"ref15","doi-asserted-by":"crossref","first-page":"592","DOI":"10.7589\/2017-09-224","article-title":"Identification of novel gammaherpesviruses in a South American fur seal (Arctocephalus australis) with ulcerative skin lesions","volume":"54","author":"Sacrist\u00e1n C","year":"2018","journal-title":"J Wildl Dis"},{"issue":"3","key":"ref16","doi-asserted-by":"crossref","first-page":"638","DOI":"10.7589\/2017-12-291","article-title":"Vasculitis and thrombosis due to the sea lion lungworm, Parafilaroides decorus, in a Guadalupe fur seal (Arctocephalus philippii townsendi)","volume":"54","author":"Seguel M","year":"2018","journal-title":"J Wildl Dis"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.jcpa.2007.04.018","article-title":"Pathological findings in harbour seals (Phoca vitulina): 1996-2005","volume":"137","author":"Siebert U","year":"2007","journal-title":"J Comp Pathol"},{"issue":"1-2","key":"ref18","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.vetpar.2010.09.022","article-title":"Identification of Hammondia heydorni oocysts by a heminested-PCR (hnPCR-AP10) based on the H. heydorni RAPD fragment AP10","volume":"175","author":"Soares RM","year":"2011","journal-title":"Vet Parasitol"},{"issue":"7","key":"ref19","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1128\/JCM.34.7.1666-1671.1996","article-title":"Detection and analysis of diverse herpesviral species by consensus primer PCR","volume":"34","author":"VanDevanter DR","year":"1996","journal-title":"J Clin Microbiol"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rbpv\/v28n3\/1984-2961-rbpv-S1984-29612019029.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,2,7]],"date-time":"2020-02-07T19:31:01Z","timestamp":1581103861000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612019000300499&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7]]},"references-count":19,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2019,7]]}},"alternative-id":["S1984-29612019000300499"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612019029","relation":{},"ISSN":["1984-2961","0103-846X"],"issn-type":[{"value":"1984-2961","type":"electronic"},{"value":"0103-846X","type":"print"}],"subject":[],"published":{"date-parts":[[2019,7]]}}}