{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T19:04:13Z","timestamp":1757703853670,"version":"3.41.2"},"reference-count":23,"publisher":"FapUNIFESP (SciELO)","issue":"1","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract The aim of this study was to confirm the emergence of canine visceral leishmaniasis among dogs in Foz do Igua\u00e7u. The disease was diagnosed through the isolation and molecular identification of Leishmania infantum. In the first sample collection stage (2012), three lymph node aspirates and 46 buffy coat samples were obtained mostly from the dogs that were seroreagents for leishmaniasis. In the second sample collection stage (2013), the buffy coat samples were collected from 376 dogs located close to Paraguay, Paran\u00e1 river, center and peripheral parts of the city. The DNA from the six isolates, four from the first sampling stage (4\/49) and two from the second sampling stage (2\/376), was subjected to polymerase chain reaction using the K26F\/R primers. The isolate was confirmed as L. infantum by sequencing. As none of the dogs had ever left the city, the isolates were confirmed as autochthonous. Further, the study confirmed the emergence of canine visceral leishmaniasis in Paran\u00e1 through the identification of L. infantum among dogs in Foz do Igua\u00e7u city. Hence, collaborative control measures should be designed and implemented by the public agencies and research institutions of Brazil, Argentina, and Paraguay to control the spread of visceral leishmaniasis.<\/jats:p>","DOI":"10.1590\/s1984-29612019083","type":"journal-article","created":{"date-parts":[[2019,11,2]],"date-time":"2019-11-02T19:36:27Z","timestamp":1572723387000},"source":"Crossref","is-referenced-by-count":3,"title":["Autochthonous canine visceral leishmaniasis cases occur in Paran\u00e1 state since 2012: isolation and identification of Leishmania infantum"],"prefix":"10.1590","volume":"29","author":[{"given":"Renata Cristina Ferreira","family":"Dias","sequence":"first","affiliation":[{"name":"Universidade Estadual de Londrina, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aline Kuhn Sbruzzi","family":"Pasquali","sequence":"additional","affiliation":[{"name":"Universidade Federal do Paran\u00e1, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanete","family":"Thomaz-Soccol","sequence":"additional","affiliation":[{"name":"Universidade Federal do Paran\u00e1, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliane Maria","family":"Pozzolo","sequence":"additional","affiliation":[{"name":"Nona Regional de Sa\u00fade do Estado do Paran\u00e1, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana","family":"Chiyo","sequence":"additional","affiliation":[{"name":"Centro de Controle de Zoonoses, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvana Maria","family":"Alban","sequence":"additional","affiliation":[{"name":"Universidade Federal do Paran\u00e1, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo Cancio","family":"Fendrich","sequence":"additional","affiliation":[{"name":"Universidade Federal do Paran\u00e1, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00f4mulo Augusto Andrade de","family":"Almeida","sequence":"additional","affiliation":[{"name":"Universidade Positivo, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda Pinto","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0475-2010","authenticated-orcid":false,"given":"Eloiza Teles","family":"Caldart","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberta Lemos","family":"Freire","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regina","family":"Mitsuka-Bregan\u00f3","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alceu","family":"Bisetto J\u00fanior","sequence":"additional","affiliation":[{"name":"Secretaria de Sa\u00fade do Estado do Paran\u00e1, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Italmar Teodorico","family":"Navarro","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2020]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1590\/S0036-46652015000200013","article-title":"Identification of Leishmania infantum in Puerto Iguaz\u00fa, Misiones, Argentina","volume":"57","author":"Acosta L","year":"2015","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1590\/S0074-02762006000100024","article-title":"Leishmania infantum versus Leishmania chagasi: do not forget the law of priority","volume":"101","author":"Dantas-Torres F","year":"2006","journal-title":"Mem Inst Oswaldo Cruz"},{"year":"2017","series-title":"EMBOSS","key":"ref3"},{"issue":"1","key":"ref4","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.diagmicrobio.2007.07.019","article-title":"Development of a molecular assay specific for the Leishmania donovani complex that discriminates L. donovani\/L. infantum zymodemes: a useful tool for typing MON-1","volume":"60","author":"Haralambous C","year":"2008","journal-title":"Diagn Microbiol Infect Dis"},{"year":"2012","series-title":"Cidades - 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