{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T20:28:56Z","timestamp":1793046536073,"version":"4.5.0"},"reference-count":36,"publisher":"FapUNIFESP (SciELO)","issue":"1","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract Heartworm disease is a health problem for dogs and cats, especially in tropical and subtropical coastal regions of the world. Some studies have compared the efficacy of the diagnostic techniques used to detect this parasitosis. Therefore, the aim of this study was to compare parasitological optical microscopy (POM), serological and molecular techniques for diagnosing canine heartworm infection. Samples were collected between July 2015 and April 2016 from 103 dogs in Cabo Frio, RJ, Brazil. The wet fresh blood, thick smears, thin smears and modified Knott\u2019s test were used to detect microfilariae. ELISA (Snap\u2122 4Dx \u00ae IDEXX) was used to detect antigens and the polymerase chain reaction (PCR) was used to detect DNA and enable sequencing for species differentiation and confirmation. 19.4% of samples were positive according to microscopy. Through PCR, 15.5% of the total were positive. Using ELISA, the positivity rate was 29.1%. Occult heartworm infection was detected in 11.6% of the samples. ELISA sensitivity was shown to be higher than PCR or microscopy (P = 0.001). Sequencing of samples confirmed the presence of Dirofilaria immitis and Acanthocheilonema reconditum . ELISA was more effective for serological diagnosis canine heartworm and should be used in clinical and epidemiological studies.<\/jats:p>","DOI":"10.1590\/s1984-29612020009","type":"journal-article","created":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T12:05:32Z","timestamp":1585656332000},"source":"Crossref","is-referenced-by-count":22,"title":["Detection of Dirofilaria immitis using microscopic, serological and molecular techniques among dogs in Cabo Frio, RJ, Brazil"],"prefix":"10.1590","volume":"29","author":[{"given":"Ta\u00edssa Ang\u00e9lica Lemos","family":"Trancoso","sequence":"first","affiliation":[{"name":"Universidade Federal Fluminense,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nath\u00e1lia da Concei\u00e7\u00e3o","family":"Lima","sequence":"additional","affiliation":[{"name":"Universidade Federal Fluminense,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alynne Silva","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Universidade Federal Fluminense,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Leles","sequence":"additional","affiliation":[{"name":"Universidade Federal Fluminense,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Beatriz Monteiro","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Universidade Federal Fluminense,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norma Vollmer","family":"Labarthe","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Otilio Machado Pereira","family":"Bastos","sequence":"additional","affiliation":[{"name":"Universidade Federal Fluminense,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7616-4560","authenticated-orcid":false,"given":"Claudia Maria Antunes","family":"Uch\u00f4a","sequence":"additional","affiliation":[{"name":"Universidade Federal Fluminense,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2020]]},"reference":[{"issue":"3-4","key":"ref1","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0304-4017(03)00077-3","article-title":"Canine dirofilariasis in the region of Concei\u00e7\u00e3o Lagoon, Florian\u00f3polis, and in the Military Police kennel, S\u00e3o Jos\u00e9, State of Santa Catarina, Brazil","volume":"113","author":"Araujo RT","year":"2003","journal-title":"Vet Parasitol"},{"issue":"9","key":"ref2","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.2460\/javma.2003.222.1221","article-title":"Comparison of results of three commercial heartworm antigen test kits 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Parasitol"},{"issue":"2","key":"ref34","doi-asserted-by":"crossref","first-page":"151","DOI":"10.4103\/0972-9062.242563","article-title":"Molecular detection of Dirofilaria immitis in dogs and mosquitoes in Tabasco, Mexico","volume":"55","author":"Torres-Chable OM","year":"2018","journal-title":"J Vector Borne Dis"},{"issue":"1-2","key":"ref35","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.vetpar.2014.01.022","article-title":"Molecular detection of co-infections with Anaplasma phagocytophilum and\/or Babesia canis canis in Dirofilaria -positive dogs from Slovakia","volume":"203","author":"V\u00edchov\u00e1 B","year":"2014","journal-title":"Vet Parasitol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1051\/parasite\/2014003","article-title":"Prevalence of canine heartworm ( Dirofilaria immitis ) disease in dogs of central Portugal","volume":"21","author":"Vieira AL","year":"2014","journal-title":"Parasite"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rbpv\/v29n1\/1984-2961-rbpv-29-1-e017219.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,23]],"date-time":"2021-01-23T14:52:19Z","timestamp":1611413539000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612020000100305&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"references-count":36,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020]]}},"alternative-id":["S1984-29612020000100305"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612020009","relation":{},"ISSN":["1984-2961","0103-846X"],"issn-type":[{"value":"1984-2961","type":"electronic"},{"value":"0103-846X","type":"print"}],"subject":[],"published":{"date-parts":[[2020]]},"article-number":"e017219"}}