{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T15:04:04Z","timestamp":1790089444854,"version":"4.0.1"},"reference-count":29,"publisher":"FapUNIFESP (SciELO)","issue":"2","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract Cryptosporidium is a zoonotic parasite that causes diarrhea in a broad range of animals, including deer. Little is known about the prevalence and genotype of Cryptosporidium spp. in P\u00e8re David\u2019s deer. In this study, 137 fecal samples from P\u00e8re David\u2019s deer were collected between July 2017 and August 2018 in the Dafeng Reserve and analyzed for Cryptosporidium spp. by nested-PCR based on the small subunit ribosomal RNA (SSU rRNA) gene, followed by sequence analyses to determine the species. The 60 kDa glycoprotein (gp60) gene was used to characterize Cryptosporidium spp. Among 137 samples, 2 (1.46%) were positive for Cryptosporidium spp. according to SSU rRNA gene sequencing results. Both samples belonged to the Cryptosporidium deer genotype, with two nucleotide deletions and one nucleotide substitution. The prevalence data and molecular characterization of this study provide basic knowledge for controlling and preventing Cryptosporidium infections in P\u00e8re David\u2019s deer in this area.<\/jats:p>","DOI":"10.1590\/s1984-29612020013","type":"journal-article","created":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T23:37:54Z","timestamp":1589931474000},"source":"Crossref","is-referenced-by-count":10,"title":["Prevalence and molecular characterization of Cryptosporidium spp. in P\u00e8re David\u2019s deer (Elaphurus davidianus) in Jiangsu, China"],"prefix":"10.1590","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1452-1299","authenticated-orcid":false,"given":"Si-Yang","family":"Huang","sequence":"first","affiliation":[{"name":"Yangzhou University,  PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Min","family":"Fan","sequence":"additional","affiliation":[{"name":"Yangzhou University,  PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Yang","sequence":"additional","affiliation":[{"name":"Yangzhou University,  PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Jun","family":"Ren","sequence":"additional","affiliation":[{"name":"Dafeng Elk National Natural Reserve,  PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing-Zhi","family":"Gong","sequence":"additional","affiliation":[{"name":"Yangzhou University,  PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Yao","sequence":"additional","affiliation":[{"name":"Yangzhou University,  PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Yang","sequence":"additional","affiliation":[{"name":"Yangzhou University,  PR 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