{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T11:32:23Z","timestamp":1781177543425,"version":"3.54.1"},"reference-count":20,"publisher":"FapUNIFESP (SciELO)","issue":"3","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract The aim of this study was to report on detection of Toxoplasma gondii DNA in oysters (Crassostrea sp.) in the state of Maranh\u00e3o. To conduct this study, 200 farmed oysters were acquired in the municipality of Raposa and 100 in Pa\u00e7o do Lumiar; and a further 100 oysters were taken from the natural stock in the municipality of Primeira Cruz. This total of 400 specimens sampled was divided into 80 pools composed of five animals each. The gills and visceral mass of each oyster were removed for DNA extraction (per pool of oysters), using a commercial kit. The nested PCR technique (with the primer SAG-1) was then used to investigate any presence of protozoa. This molecular technique demonstrated the presence of DNA of T. gondii in 2.5% of the pools of oysters (n = 2\/80): these oysters were exclusively from farms. The results from this study allow the conclusion that oysters of the genus Crassostrea that are farmed in the state of Maranh\u00e3o are capable of filtering oocysts of T. gondii and maintaining them in their tissues. They are therefore potential sources of contamination for humans and other animals.<\/jats:p>","DOI":"10.1590\/s1984-29612020050","type":"journal-article","created":{"date-parts":[[2020,7,12]],"date-time":"2020-07-12T16:53:07Z","timestamp":1594572787000},"source":"Crossref","is-referenced-by-count":10,"title":["First report of detection of Toxoplasma gondii DNA in oysters (Crassostrea sp.) in the state of Maranh\u00e3o"],"prefix":"10.1590","volume":"29","author":[{"given":"Camila Moraes","family":"Silva","sequence":"first","affiliation":[{"name":"Universidade Estadual do Maranh\u00e3o,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Let\u00edcia Pinto","family":"Silva","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Maranh\u00e3o,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karinne Francisca Cardoso","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Maranh\u00e3o,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3970-7524","authenticated-orcid":false,"given":"Nancyleni Pinto","family":"Chaves Bezerra","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Maranh\u00e3o,  Brasil; 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