{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T16:35:53Z","timestamp":1777480553948,"version":"3.51.4"},"reference-count":50,"publisher":"FapUNIFESP (SciELO)","issue":"4","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract Natural products are ecofriendly agents that can be used against parasitic diseases. Eimeria species cause eimeriosis in many birds and mammals and resistance to available medications used in the treatment of eimeriosis is emerging. We investigated the in vitro and in vivo activity of Morus nigra leaf extracts (MNLE) against sporulation of oocysts and infection of mice with Eimeria papillata. Phytochemical analysis of MNLE showed the presence of seven compounds and the in vitro effects of MNLE, amprolium, DettolTM, formalin, ethanol, and phenol were studied after incubation with oocysts before sporulation. Furthermore, infection of mice with E. papillata induced an oocyst output of approximately 12 \u00d7 105 oocysts\/g of feces. MNLE significantly decreased oocyst output to approximately 86% and the total number of parasitic stages in the jejunum by approximately 87%. In addition, the reduction in the number of goblet cells in the jejuna of mice was increased after treatment. These findings suggest that mulberry exhibited powerful anticoccidial activity.<\/jats:p>","DOI":"10.1590\/s1984-29612020072","type":"journal-article","created":{"date-parts":[[2020,10,21]],"date-time":"2020-10-21T19:09:10Z","timestamp":1603307350000},"source":"Crossref","is-referenced-by-count":17,"title":["Mulberry extract as an ecofriendly anticoccidial agent: in vitro and in vivo application"],"prefix":"10.1590","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4225-2463","authenticated-orcid":false,"given":"Felwa Abdullah","family":"Thagfan","sequence":"first","affiliation":[{"name":"Princess Nourah Bint Abdulrahman University,  Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wafa Abdullah","family":"Al-Megrin","sequence":"additional","affiliation":[{"name":"Princess Nourah Bint Abdulrahman University,  Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saleh","family":"Al-Quraishy","sequence":"additional","affiliation":[{"name":"King Saud University,  Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed Abdel Monem","family":"Dkhil","sequence":"additional","affiliation":[{"name":"King Saud University,  Saudi Arabia; Helwan University,  Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2020]]},"reference":[{"issue":"25","key":"ref1","doi-asserted-by":"crossref","first-page":"889","DOI":"10.5897\/JMPR2010.733","article-title":"Ethnobotanical and taxonomic screening of genus Morus for wild edible fruits used by the inhabitants of Lesser Himalayas-Pakistan","volume":"8","author":"Abbasi AM","year":"2014","journal-title":"J Med Plants Res"},{"issue":"4","key":"ref2","first-page":"217","article-title":"The effect of a mixture of herbal essential oils, an organic acid or a probiotic on broiler performance","volume":"34","author":"Al\u00e7i\u00e7ek A","year":"2004","journal-title":"S Afr J Anim Sci"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"71","DOI":"10.3109\/00365528609093820","article-title":"The role of mucus in the protection of the gastroduodenal mucosa","volume":"21","author":"Allen A","year":"1986","journal-title":"Scand J Gastroenterol Suppl"},{"key":"ref4","article-title":"Antioxidant and anti-inflammatory activities of pomegranate (Punica granatum) on Eimeria papillata-induced infection in mice","volume":"2015","author":"Amer OS","year":"2015","journal-title":"BioMed Res Int"},{"key":"ref5","series-title":"Bovine medicine disease and husbandry of cattle","author":"Andrews AH","year":"2004","edition":"2nd"},{"issue":"3","key":"ref6","doi-asserted-by":"crossref","first-page":"501","DOI":"10.3382\/ps.2013-03634","article-title":"Milestones in avian coccidiosis research: a review","volume":"93","author":"Chapman HD","year":"2014","journal-title":"Poult Sci"},{"issue":"4","key":"ref7","article-title":"Anti-inflammatory and antinociceptive properties of flavonoids from the fruits of black mulberry (Morus nigra L.)","volume":"11","author":"Chen H","year":"2016","journal-title":"PLoS One"},{"issue":"4","key":"ref8","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1002\/aja.1001410404","article-title":"Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine. 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