{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T08:21:04Z","timestamp":1769156464029,"version":"3.49.0"},"reference-count":44,"publisher":"FapUNIFESP (SciELO)","issue":"4","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract The aim of this study was to investigate rickettsial infection in equids, opossums and ticks in the municipality of Monte Mor, a place where a Brazilian spotted fever case occurred in 2005. In addition, characteristics possibly associated with seropositivity in horses were analyzed. Serum samples from horses, mules and opossums (Didelphis albiventris) were subjected to indirect immunofluorescence assay (IFA) against Rickettsia rickettsii. The ticks collected from the animals were identified and Amblyomma sculptum ticks from the equids were tested using PCR for Rickettsia spp. Anti-R. rickettsii antibodies were detected in 22.6% (14\/62) of the horses, none of the mules and 21.7% (5\/23) of the opossums. Among the variables analyzed, only age &gt; 12 years showed a statistically significant association with seropositivity among horses. All of the 166 A. sculptum ticks tested using PCR were negative. The results showed that rickettsiae of the spotted fever group was circulating in the municipality of Monte Mor when the samples were collected and indicate a need for surveillance of Brazilian spotted fever in this region.<\/jats:p>","DOI":"10.1590\/s1984-29612020073","type":"journal-article","created":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T04:05:36Z","timestamp":1606190736000},"source":"Crossref","is-referenced-by-count":9,"title":["Rickettsial infection in equids, opossums and ticks in the municipality of Monte Mor, state of S\u00e3o Paulo, Brazil"],"prefix":"10.1590","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6141-6734","authenticated-orcid":false,"given":"Tatiana Evelyn Hayama","family":"Ueno","sequence":"first","affiliation":[{"name":"Ag\u00eancia Paulista de Tecnologia dos Agroneg\u00f3cios,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 Antonio","family":"Cutolo","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thiago Fernandes","family":"Martins","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonas","family":"Moraes-Filho","sequence":"additional","affiliation":[{"name":"Universidade Santo Amaro,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e9rgio Santos de","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Universidade Federal de Campina Grande,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo Bahia","family":"Labruna","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2020]]},"reference":[{"issue":"6","key":"ref1","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1603\/ME14042","article-title":"Seroprevalence of Rickettsia spp. in equids and molecular detection of \u201cCandidatus Rickettsia amblyommii\u201d in Amblyomma cajennense sensu lato ticks from the Pantanal region of Mato Grosso, Brazil","volume":"51","author":"Alves AS","year":"2014","journal-title":"J Med Entomol"},{"key":"ref2","series-title":"Carrapatos de import\u00e2ncia m\u00e9dico-veterin\u00e1ria da Regi\u00e3o Neotropical: um guia ilustrado para identifica\u00e7\u00e3o de esp\u00e9cies","author":"Barros-Battesti DM","year":"2006"},{"key":"ref3","series-title":"Casos confirmados notificados no Sistema de Informa\u00e7\u00e3o de Agravos de Notifica\u00e7\u00e3o \u2013 S\u00e3o Paulo","year":"2019"},{"issue":"4","key":"ref4","doi-asserted-by":"crossref","first-page":"575","DOI":"10.2307\/3284227","article-title":"Parasitology meets ecology on its own terms: Margolis et al. revisited","volume":"83","author":"Bush AO","year":"1997","journal-title":"J Parasitol"},{"issue":"4","key":"ref5","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.vetpar.2003.09.004","article-title":"Acquired resistance of horses to Amblyomma cajennense (Fabricius, 1787) ticks","volume":"117","author":"Castagnolli KC","year":"2003","journal-title":"Vet Parasitol"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1093\/jme\/tjz141","article-title":"Rickettsia rickettsii (Rickettsiales: Rickettsiaceae) infecting Amblyomma sculptum (Acari: Ixodidae) ticks and capybaras in a Brazilian spotted fever-endemic area of Brazil","volume":"57","author":"Costa FB","year":"2020","journal-title":"J Med 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Vet"},{"issue":"3","key":"ref10","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1179\/000349804225003316","article-title":"The distribution and ecological \u2018preferences\u2019 of the tick Amblyomma cajennense (Acari: Ixodidae), an ectoparasite of humans and other mammals in the Americas","volume":"98","author":"Estrada-Pe\u00f1a A","year":"2004","journal-title":"Ann Trop Med Parasitol"},{"issue":"8","key":"ref11","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1590\/S0074-02762005000800004","article-title":"Detection of Rickettsia rickettsii in the tick Amblyomma cajennense in a new Brazilian spotted fever endemic area in the state of Minas Gerais","volume":"100","author":"Guedes E","year":"2005","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"7","key":"ref12","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1590\/S0074-02762007000700003","article-title":"Rickettsia infection in five areas of the state of S\u00e3o Paulo, Brazil","volume":"102","author":"Horta MC","year":"2007","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1089\/vbz.2008.0114","article-title":"Experimental infection of opossums Didelphis aurita by Rickettsia rickettsii and evaluation of the transmission of the infection to ticks Amblyomma cajennense.","volume":"9","author":"Horta MC","year":"2009","journal-title":"Vector Borne Zoonotic Dis"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"93","DOI":"10.4269\/ajtmh.2004.71.93","article-title":"Prevalence of antibodies to spotted fever group rickettsiae in humans and domestic animals in a Brazilian spotted fever-endemic area in the state of S\u00e3o Paulo, Brazil: serologic evidence for infection by Rickettsia rickettsii and another spotted fever group Rickettsia.","volume":"71","author":"Horta MC","year":"2004","journal-title":"Am J Trop Med Hyg"},{"key":"ref15","series-title":"Censo agropecu\u00e1rio 2017: n\u00famero de cabe\u00e7as de muares nos estabelecimentos agropecu\u00e1rios","year":"2017"},{"key":"ref16","series-title":"Invent\u00e1rio florestal do estado de S\u00e3o Paulo","year":"2009"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0304-4017(01)00387-9","article-title":"Risk factors to tick infestations and their occurrence on horses in the state of S\u00e3o Paulo, Brazil","volume":"97","author":"Labruna MB","year":"2001","journal-title":"Vet Parasitol"},{"issue":"6","key":"ref18","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1093\/jmedent\/45.6.1156","article-title":"Comparative susceptibility of larval stages of Amblyomma aureolatum, Amblyomma cajennense, and Rhipicephalus sanguineus to infection by Rickettsia rickettsii.","volume":"45","author":"Labruna MB","year":"2008","journal-title":"J Med 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An overview of reported statistical methods and appropriateness of interpretations in cross-sectional studies with dichotomous outcomes in Veterinary Medicine","volume":"4","author":"Martinez BAF","year":"2017","journal-title":"Front Vet Sci"},{"issue":"2","key":"ref22","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.ttbdis.2010.03.002","article-title":"Nymphs of the genus Amblyomma (Acari: Ixodidae) of Brazil: descriptions, redescriptions, and identification key","volume":"1","author":"Martins TF","year":"2010","journal-title":"Ticks Tick Borne Dis"},{"issue":"2","key":"ref23","doi-asserted-by":"crossref","first-page":"50","DOI":"10.4322\/rbpv.01802011","article-title":"Ocorr\u00eancia de carrapatos (Acari: Ixodidae) no munic\u00edpio de Goiatins, Tocantins","volume":"18","author":"Martins TF","year":"2009","journal-title":"Rev Bras Parasitol Vet"},{"key":"ref24","series-title":"A febre maculosa brasileira na Regi\u00e3o Metropolitana de Campinas\/SP: sua distribui\u00e7\u00e3o espacial e os desafios das a\u00e7\u00f5es de preven\u00e7\u00e3o e controle locais","author":"Nasser JT","year":"2014"},{"issue":"3","key":"ref25","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.ttbdis.2013.11.004","article-title":"Reassessment of the taxonomic status of Amblyomma cajennense (Fabricius, 1787) with the description of three new species, Amblyomma tonelliae n. sp., Amblyomma interandinum n. sp. and Amblyomma patinoi n. sp., and reinstatement of Amblyomma mixtum Koch, 1844, and Amblyomma sculptum Berlese, 1888 (Ixodida: ixodidae)","volume":"5","author":"Nava S","year":"2014","journal-title":"Ticks Tick Borne Dis"},{"issue":"4","key":"ref26","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1007\/s10493-009-9331-6","article-title":"Hosts, distribution and genetic divergence (16S rDNA) of Amblyomma dubitatum (Acari: ixodidae)","volume":"51","author":"Nava S","year":"2010","journal-title":"Exp Appl Acarol"},{"issue":"4","key":"ref27","article-title":"Brazilian spotted fever serological survey among equids at the Guarapiranga Dam area in the city of S\u00e3o Paulo, Brazil","volume":"56","author":"Oliveira AS","year":"2019","journal-title":"Braz J Vet Res Anim Sci"},{"issue":"4","key":"ref28","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1590\/s1984-29612016060","article-title":"Probability of occurrence of the Brazilian spotted fever in northeast of Paran\u00e1 state, Brazil","volume":"25","author":"Otomura FH","year":"2016","journal-title":"Rev Bras Parasitol Vet"},{"issue":"4","key":"ref29","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1128\/CMR.00032-13","article-title":"Update on tick-borne rickettsioses around the world: a geographic approach","volume":"26","author":"Parola P","year":"2013","journal-title":"Clin Microbiol Rev"},{"issue":"6","key":"ref30","doi-asserted-by":"crossref","DOI":"10.1016\/j.ttbdis.2019.07.005","article-title":"Multi-locus phylogenetic analysis groups the New World bacterium Rickettsia sp. Strain ApPR with the Old World species R. africae; proposal of \u201cCandidatus Rickettsia paranaensis\u201d","volume":"10","author":"Peckle M","year":"2019","journal-title":"Ticks Tick Borne Dis"},{"issue":"4","key":"ref31","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1590\/S1984-29612008000400008","article-title":"Carrapatos do g\u00eanero Amblyomma (Acari: Ixodidae) e suas rela\u00e7\u00f5es com os hospedeiros em \u00e1rea end\u00eamica para febre maculosa no Estado de S\u00e3o Paulo","volume":"17","author":"Perez CA","year":"2008","journal-title":"Rev Bras Parasitol Vet"},{"key":"ref32","series-title":"R: a language and environment for statistical computing","year":"2019"},{"issue":"4","key":"ref33","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1128\/CMR.10.4.694","article-title":"Rickettsioses as paradigms of new or emerging infectious diseases","volume":"10","author":"Raoult D","year":"1997","journal-title":"Clin Microbiol Rev"},{"issue":"2","key":"ref34","doi-asserted-by":"crossref","first-page":"265","DOI":"10.3201\/eid1102.040656","article-title":"Rickettsial infection in animals and Brazilian spotted fever endemicity","volume":"11","author":"Sangioni LA","year":"2005","journal-title":"Emerg Infect Dis"},{"issue":"2","key":"ref35","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/s10493-012-9570-9","article-title":"Ticks (Acari: Ixodidae) associated with small terrestrial mammals in the state of Minas Gerais, southeastern Brazil","volume":"58","author":"Saraiva DG","year":"2012","journal-title":"Exp Appl Acarol"},{"issue":"7","key":"ref36","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1111\/zph.12180","article-title":"Rickettsial infection in animals, humans and ticks in Paulic\u00e9ia, Brazil","volume":"62","author":"Silveira I","year":"2015","journal-title":"Zoonoses Public Health"},{"issue":"2","key":"ref37","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1111\/j.1365-2915.2011.00982.x","article-title":"Experimental infection of the tick Amblyomma cajennense, Cayenne tick, with Rickettsia rickettsii, the agent of Rocky Mountain spotted fever","volume":"26","author":"Soares JF","year":"2012","journal-title":"Med Vet Entomol"},{"issue":"4","key":"ref38","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0153303","article-title":"High seroprevalence for Rickettsia rickettsii in equines suggests risk of human infection in silent areas for the Brazilian spotted fever","volume":"11","author":"Souza CE","year":"2016","journal-title":"PLoS One"},{"issue":"3","key":"ref39","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/s10493-014-9877-9","article-title":"Association patterns of ticks (Acari: Ixodida: Ixodidae, Argasidae) of small mammals in Cerrado fragments, western Brazil","volume":"65","author":"Sponchiado J","year":"2015","journal-title":"Exp Appl Acarol"},{"key":"ref40","series-title":"epiR: tools for the Analysis of Epidemiological Data. R package version 1.0-11","author":"Stevenson M","year":"2020"},{"key":"ref41","first-page":"27","article-title":"Ecology, biology and distribution of spotted-fever tick vectors in Brazil","volume":"3","author":"Szab\u00f3 MPJ","year":"2013","journal-title":"Front Cell Infect Microbiol"},{"key":"ref42","article-title":"Gastrointestinal, skin and blood parasites in Didelphis spp. from urban and sylvatic areas in S\u00e3o Paulo state, Brazil","volume":"16","author":"Teodoro AKM","year":"2019","journal-title":"Vet Parasitol Reg Stud Rep"},{"issue":"1","key":"ref43","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1186\/s13071-016-1784-y","article-title":"Experimental infection of horses with Rickettsia rickettsii.","volume":"9","author":"Ueno TE","year":"2016","journal-title":"Parasit Vectors"},{"issue":"1","key":"ref44","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1741-7007-7-6","article-title":"Evolution and diversity of Rickettsia bacteria","volume":"7","author":"Weinert LA","year":"2009","journal-title":"BMC Biol"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rbpv\/v29n4\/1984-2961-rbpv-29-4-e015420.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,24]],"date-time":"2021-01-24T01:19:03Z","timestamp":1611451143000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612020000400317&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"references-count":44,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020]]}},"alternative-id":["S1984-29612020000400317"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612020073","relation":{},"ISSN":["1984-2961","0103-846X"],"issn-type":[{"value":"1984-2961","type":"electronic"},{"value":"0103-846X","type":"print"}],"subject":[],"published":{"date-parts":[[2020]]},"article-number":"e015420"}}