{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:17:04Z","timestamp":1753888624876,"version":"3.41.2"},"reference-count":25,"publisher":"FapUNIFESP (SciELO)","issue":"4","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract This study aimed to verify the presence of IgG antibodies against Borrelia burgdorferi sensu lato (s.l) in domestic dogs in western Cuba. Serum samples were analyzed by indirect enzyme-linked immunosorbent assay (ELISA), using crude antigens of a B. burgdorferi strain of North American origin. To verify the presence of Borrelia spp., deoxyribonucleic acid (DNA) extracted from individual blood samples was analyzed by nested-PCR, with markers targeted for amplification of portions of the flagellin B gene (flaB) present in Borrelia spirochetes. Ticks were also collected through inspection of the animals. Sera from 93 of 176 (52.84%) dogs were reactive to the indirect ELISA. Geographic prevalence varied from 54.35% (25\/46) in Boyeros, 44.44% (20\/45) in Cotorro, 66.67% (22\/33) in Habana del Este, and 50% (26\/52) in San Jos\u00e9 de las Lajas. There was no statistical difference between these tested variables. No blood samples analyzed were positive for the Borrelia flaB gene.<\/jats:p>","DOI":"10.1590\/s1984-29612020104","type":"journal-article","created":{"date-parts":[[2020,12,6]],"date-time":"2020-12-06T22:18:07Z","timestamp":1607293087000},"source":"Crossref","is-referenced-by-count":0,"title":["First serological detection of Borrelia spp. in dogs in western Cuba"],"prefix":"10.1590","volume":"29","author":[{"given":"Matheus Dias","family":"Cordeiro","sequence":"first","affiliation":[{"name":"Universidade Federal Rural do Rio de Janeiro,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia Bezerra da","family":"Silva","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural do Rio de Janeiro,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maylin Gonzalez","family":"Navarrete","sequence":"additional","affiliation":[{"name":"Universidad Agraria de La Habana,  Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eug\u00eanio","family":"Roque","sequence":"additional","affiliation":[{"name":"Universidad Agraria de La Habana,  Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5834-141X","authenticated-orcid":false,"given":"Adivaldo Henrique da","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural do Rio de Janeiro,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2020]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.rvsc.2006.11.014","article-title":"Isolation and molecular characterization of a Brazilian strain of Borrelia anserina, the agent of fowl spirochaetosis","volume":"83","author":"Ataliba AC","year":"2007","journal-title":"Res Vet Sci"},{"key":"ref2","series-title":"Carrapatos de import\u00e2ncia m\u00e9dico-veterin\u00e1ria da regi\u00e3o neotropical: um guia ilustrado para identifica\u00e7\u00e3o de esp\u00e9cies.","author":"Barros-Battesti DM","year":"2006","edition":"1."},{"issue":"2","key":"ref3","first-page":"101","article-title":"The ixodid ticks (Acari: Ixodidae) of Cuba","volume":"142","author":"Barros-Battesti DM","year":"2009","journal-title":"Syst Appl Acarol"},{"issue":"1-2","key":"ref4","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.vetpar.2008.10.093","article-title":"Prevalence and geographic distribution of Dirofilaria immitis, Borrelia burgdorferi, Ehrlichia canis, and Anaplasma phagocytophilum in dogs in the United States: results of a national clinic- based serologic survey","volume":"160","author":"Bowman D","year":"2009","journal-title":"Vet Parasitol"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/s10493-019-00435-8","article-title":"Borrelia burgdorferi sensu lato infecting Ixodes auritulus ticks in Uruguay","volume":"80","author":"Carvalho LA","year":"2020","journal-title":"Exp Appl Acarol"},{"issue":"4","key":"ref6","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1590\/s1984-296120180083","article-title":"Morphological, molecular and phylogenetic characterization of Borrelia theileri in Rhipicephalus microplus.","volume":"27","author":"Cordeiro MD","year":"2018","journal-title":"Rev Bras Parasitol Vet"},{"issue":"3","key":"ref7","first-page":"251","article-title":"Soropreval\u00eancia para Borrelia spp. em c\u00e3es no munic\u00edpio de Serop\u00e9dica, estado do Rio de Janeiro","volume":"34","author":"Cordeiro MD","year":"2012","journal-title":"Rev Bras Med Vet"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1186\/s13071-019-3735-x","article-title":"Trends in canine seroprevalence to Borrelia burgdorferi and Anaplasma spp. in the eastern USA, 2010-2017","volume":"12","author":"Dewage BG","year":"2019","journal-title":"Parasit Vectors"},{"issue":"4","key":"ref9","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.pt.2017.12.006","article-title":"The blacklegged tick, Ixodes scapularis: an increasing public health concern","volume":"34","author":"Eisen RJ","year":"2018","journal-title":"Trends Parasitol"},{"issue":"1-2","key":"ref10","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0022-1759(98)00170-7","article-title":"A statistically defined endpoint titer determination method for immunoassays","volume":"221","author":"Frey A","year":"1998","journal-title":"J Immunol Methods"},{"issue":"1","key":"ref11","first-page":"3","article-title":"Acerca de la enfermedad de Lyme en Cuba y su conocimiento en personal m\u00e9dico","volume":"44","author":"Gonz\u00e1lez IR","year":"2018","journal-title":"Rev Cuba Salud P\u00fablica"},{"issue":"5","key":"ref12","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1590\/S0036-46652001000500003","article-title":"Borrelia burgdorferi antibodies in dogs from Cotia county, S\u00e3o Paulo State, Brazil","volume":"43","author":"Joppert AM","year":"2001","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"issue":"6","key":"ref13","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/j.rbr.2017.04.001","article-title":"Evid\u00eancia molecular de Borrelia burgdorferi sensu lato em pacientes no centro\u2010oeste Brasileiro","volume":"57","author":"Lopes FA","year":"2017","journal-title":"Rev Bras Reumatol"},{"issue":"3","key":"ref14","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1590\/S0036-46652012000300007","article-title":"Amplification of the flgE gene provides evidence for the existence of a Brazilian borreliosis","volume":"54","author":"Mantovani E","year":"2012","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"issue":"4","key":"ref15","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1016\/j.ttbdis.2018.03.008","article-title":"Isolation and molecular characterization of a relapsing fever Borrelia recovered from Ornithodoros rudis in Brazil","volume":"9","author":"Mu\u00f1oz-Leal S","year":"2018","journal-title":"Ticks Tick Borne Dis"},{"key":"ref16","first-page":"63","article-title":"Molecular detection of Ehrlichia canis and Babesia canis vogeli in Rhipicephalus sanguineus sensu lato ticks from Cuba","volume":"38","author":"Navarrete MG","year":"2016","journal-title":"Rev Bras Med Vet"},{"issue":"8","key":"ref17","doi-asserted-by":"crossref","first-page":"616","DOI":"10.3390\/pathogens9080616","article-title":"Ticks and tick-borne diseases in Cuba, half a century of scientific research","volume":"9","author":"Obreg\u00f3n Alvarez D","year":"2020","journal-title":"Pathogens"},{"issue":"9","key":"ref18","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.3201\/eid1009.031048","article-title":"Do antiborrelial antibodies suggest Lyme disease in Cuba?","volume":"10","author":"Rodr\u00edguez I","year":"2004","journal-title":"Emerg Infect Dis"},{"issue":"1","key":"ref19","first-page":"82","article-title":"Prevalence of antibodies to Borrelia burgdorferi sensu stricto in humans from a Cuban village","volume":"16","author":"Rodr\u00edguez I","year":"2012","journal-title":"Braz J Infect Dis"},{"issue":"5","key":"ref20","first-page":"164","article-title":"Evidences about human tick-borne infections in Cuba","volume":"3","author":"Rodr\u00edguez I","year":"2018","journal-title":"Acta Biom Scient"},{"issue":"1","key":"ref21","first-page":"41","article-title":"Lyme disease in Cuba? Report of possible cases","volume":"55","author":"Rodr\u00edguez I","year":"2003","journal-title":"Rev Cubana Med Trop"},{"issue":"5","key":"ref22","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1016\/j.ttbdis.2016.04.012","article-title":"Molecular detection and characterization of Anaplasma platys in dogs and ticks in Cuba","volume":"7","author":"Silva CB","year":"2016","journal-title":"Ticks Tick Borne Dis"},{"issue":"4","key":"ref23","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1007\/s11250-017-1504-4","article-title":"Epidemiological investigation of Borrelia burgdorferi in horses in the municipality of Sinop-MT, Brazil","volume":"50","author":"Socoloski SNG","year":"2018","journal-title":"Trop Anim Health Prod"},{"issue":"1","key":"ref24","first-page":"55","article-title":"Enzyme immunoassays in diagnostic medicine: theory and practice","volume":"53","author":"Voller A","year":"1976","journal-title":"Bull World Health Organ"},{"issue":"3","key":"ref25","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1590\/S0074-02762003000300004","article-title":"Coexistence of antibodies to tick-borne agents of babesiosis and Lyme borreliosis in patients from Cotia County, State of Sao Paulo, Brazil","volume":"98","author":"Yoshinari NH","year":"2003","journal-title":"Mem Inst Oswaldo Cruz"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rbpv\/v29n4\/1984-2961-rbpv-29-4-e014020.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,24]],"date-time":"2021-01-24T00:27:19Z","timestamp":1611448039000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612020000400407&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"references-count":25,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020]]}},"alternative-id":["S1984-29612020000400407"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612020104","relation":{},"ISSN":["1984-2961","0103-846X"],"issn-type":[{"type":"electronic","value":"1984-2961"},{"type":"print","value":"0103-846X"}],"subject":[],"published":{"date-parts":[[2020]]},"article-number":"e014020"}}