{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T19:47:34Z","timestamp":1765828054014,"version":"3.41.2"},"reference-count":40,"publisher":"FapUNIFESP (SciELO)","issue":"2","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract The present study aimed to evaluate a methodology for active surveillance of visceral leishmaniasis by detecting Leishmania DNA in organs of wild road-killed animals from November 2016 to October 2018 in the North of Paran\u00e1, Brazil. The collection points of road-killed wild animals were georeferenced. The animals were autopsied and samples of bone marrow, lymph node, liver, spleen, and ear skin were collected. Genomic DNA was extracted and subjected to PCR for amplification of Leishmania spp. 18S, kinetoplastic DNA (kDNA), HSP70, and ITS1 genes, and DNA sequencing was performed. The primers used for the amplification of kDNA, ITS1, and HSP70 genes presented non-specific results. Of the 66 mammals collected from 24 different municipalities, one Southern Tamandua (Tamandua tetradactyla) presented DNA of Leishmania spp. in lymph nodes by 18S PCR. DNA sequencing confirmed the results of the subgenus, Viannia, identification. We suggest using the methodology showed in the present study in the active and early surveillance of visceral leishmaniasis in a non-endemic area.<\/jats:p>","DOI":"10.1590\/s1984-29612021026","type":"journal-article","created":{"date-parts":[[2021,5,10]],"date-time":"2021-05-10T12:32:40Z","timestamp":1620649960000},"source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of an active and early surveillance methodology for visceral leishmaniasis by molecular detection in road-killed wild fauna"],"prefix":"10.1590","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0475-2010","authenticated-orcid":false,"given":"Eloiza Teles","family":"Caldart","sequence":"first","affiliation":[{"name":"Universidade Estadual de Londrina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda","family":"Pinto-Ferreira","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andressa Maria Rorato Nascimento de","family":"Matos","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aline Ticiani Pereira","family":"Pascoal","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda","family":"Bert\u00e3o-Santos","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regina","family":"Mitsuka-Bregan\u00f3","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Italmar Teodorico","family":"Navarro","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina,  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silvestres dos Estados do Mato Grosso e Par\u00e1","author":"Soares HS","key":"ref37"},{"issue":"6","key":"ref38","first-page":"1041","article-title":"First Report of Autochthonous Visceral Leishmaniosis in humans in Foz do Igua\u00e7u, Paran\u00e1 State, Southern Brazil","volume":"2","author":"Trench FJP","year":"2016","journal-title":"Ann Clin Cytol Pathol"},{"issue":"2","key":"ref39","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1128\/CMR.00104-14","article-title":"Species typing in dermal leishmaniasis","volume":"28","author":"Van der Auwera G","year":"2015","journal-title":"Clin Microbiol Rev"},{"issue":"1","key":"ref40","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0166-6851(92)90208-2","article-title":"Sequence analysis of small subunit ribosomal RNA genes and its use for detection and identification of Leishmania parasites","volume":"51","author":"Van Eys GJJM","year":"1992","journal-title":"Mol Biochem Parasitol"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rbpv\/v30n2\/1984-2961-rbpv-30-2-e027920.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,11]],"date-time":"2021-05-11T18:53:29Z","timestamp":1620759209000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612021000200302&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"references-count":40,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2021]]}},"alternative-id":["S1984-29612021000200302"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612021026","relation":{},"ISSN":["1984-2961","0103-846X"],"issn-type":[{"type":"electronic","value":"1984-2961"},{"type":"print","value":"0103-846X"}],"subject":[],"published":{"date-parts":[[2021]]},"article-number":"e027920"}}