{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T08:17:34Z","timestamp":1758961054196,"version":"3.41.2"},"reference-count":42,"publisher":"FapUNIFESP (SciELO)","issue":"2","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract The aim of this study was to evaluate the genotypic characteristics of Toxoplasma gondii isolated from free-range chickens in the metropolitan area of Goi\u00e2nia, Goi\u00e1s, in Brazil\u2019s central-west region. The seroprevalence rate was found to be 96%, according to an indirect hemagglutination assay. Brain and heart samples were processed by peptic digestion for a mice bioassay. The tissues were homogenized and the resulting samples were subjected to polymerase chain reaction (PCR), which revealed that 64% of them contained the parasite's DNA. The mice bioassay revealed 15 isolates, 8 of them tachyzoites isolates from the peritoneal lavage and 7 from brain cysts. T. gondii genotypes were determined through PCR-RFLP, using the following markers: SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, alt. SAG2, Apico and CS3. Three genotypes were identified, inclued ToxoDB #65, and the other two are not yet described in the literature. Hence, we conclude that the isolates obtained from the metropolitan area of Goi\u00e2nia showed relatively low genetic diversity.<\/jats:p>","DOI":"10.1590\/s1984-29612021029","type":"journal-article","created":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T18:03:23Z","timestamp":1622225003000},"source":"Crossref","is-referenced-by-count":3,"title":["Molecular characterization of Toxoplasma gondii isolates from free-range chickens reveals new genotypes in Goi\u00e2nia, Goi\u00e1s, Brazil"],"prefix":"10.1590","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5816-1427","authenticated-orcid":false,"given":"Hanstter Hallison Alves","family":"Rezende","sequence":"first","affiliation":[{"name":"Universidade Federal de Jata\u00ed,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaqueline Ata\u00edde Silva Lima da","family":"Igreja","sequence":"additional","affiliation":[{"name":"Universidade Federal de Goi\u00e1s,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ant\u00f4nio Roberto","family":"Gomes-J\u00fanior","sequence":"additional","affiliation":[{"name":"Universidade Federal de Goi\u00e1s,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jade de Oliveira","family":"Melo","sequence":"additional","affiliation":[{"name":"Universidade Federal de Goi\u00e1s,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Lu\u00eds","family":"Garcia","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felippe Danyel Cardoso","family":"Martins","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helo\u00edsa Ribeiro","family":"Storchilo","sequence":"additional","affiliation":[{"name":"Universidade Federal de Goi\u00e1s,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taynara Cristina","family":"Gomes","sequence":"additional","affiliation":[{"name":"Universidade Federal de Goi\u00e1s,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina Clare","family":"Vinaud","sequence":"additional","affiliation":[{"name":"Universidade Federal de Goi\u00e1s,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Maria de","family":"Castro","sequence":"additional","affiliation":[{"name":"Universidade Federal de Goi\u00e1s,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2021]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1645\/GE-1829.1","article-title":"Seroepidemiology of Toxoplasma gondii infection in pregnant women in rural Durango, Mexico","volume":"95","author":"Alvarado-Esquivel C","year":"2009","journal-title":"J 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goiano","volume":"10","author":"Silveira No OJ","year":"2014","journal-title":"Encicl Biosf"},{"year":"2013","series-title":"Perfil soroepidemiol\u00f3gico e detec\u00e7\u00e3o molecular de Toxoplasma gondii em Gallus gallus e Columba livia da regi\u00e3o metropolitana de Goi\u00e2nia, Goi\u00e1s","author":"Silveira No OJ","key":"ref38"},{"issue":"1","key":"ref39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0031182009991065","article-title":"Moving towards an integrated approach to molecular detection and identification of Toxoplasma gondii.","volume":"137","author":"Su C","year":"2010","journal-title":"Parasitology"},{"issue":"1","key":"ref40","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1590\/1678-4162-8594","article-title":"Genotyping of Toxoplasma gondii isolates from naturally infected Gallus domesticus in Santa Catarina state, Brazil","volume":"69","author":"Trevisani N","year":"2017","journal-title":"Arq Bras Med Vet 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