{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:11:29Z","timestamp":1790644289014,"version":"4.1.0"},"reference-count":26,"publisher":"FapUNIFESP (SciELO)","issue":"3","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract Trichinellosis is a zoonosis caused by nematodes of the Trichinella genus, of which 10 species have been described. Species identification when larvae is found is only possible using molecular tools. Meat from pigs and game animals not subjected to veterinary inspection are the main sources of human infections. The hunting of native carnivores is prohibited in Chile due to conservation issues and the fact that those animals favor pest control. The illegal hunting of a cougar (Puma concolor) occurred in September 2020. Herein, the molecular identification of Trichinella larvae, by analyzing nuclear (expansion segment V) and mitochondrial (cytochrome C oxidase subunit I) sequences are described. Both the amplification of the expansion segment V region and the phylogenetic analysis of a segment of a fragment of the cytochrome c-oxidase subunit I sequence confirmed that the larvae belonged to T. spiralis. The case described herein represents the first evidence of illegal hunting of a protected mammal infected with Trichinella in Chile, highlighting the \u2018One Health\u2019 perspective to face this disease in the rural-sylvatic interphase.<\/jats:p>","DOI":"10.1590\/s1984-29612021033","type":"journal-article","created":{"date-parts":[[2021,5,24]],"date-time":"2021-05-24T20:24:10Z","timestamp":1621887850000},"source":"Crossref","is-referenced-by-count":11,"title":["Trichinella spiralis in a cougar (Puma concolor) hunted by poachers in Chile"],"prefix":"10.1590","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1704-5833","authenticated-orcid":false,"given":"Diana Maritza","family":"Echeverry","sequence":"first","affiliation":[{"name":"Universidad de Concepci\u00f3n,  Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3133-8616","authenticated-orcid":false,"given":"Adriana Milena Santodomingo","family":"Santodomingo","sequence":"additional","affiliation":[{"name":"Universidad de Concepci\u00f3n,  Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5506-4461","authenticated-orcid":false,"given":"Richard Said","family":"Thomas","sequence":"additional","affiliation":[{"name":"Universidad de Concepci\u00f3n,  Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Gonz\u00e1lez-Ug\u00e1s","sequence":"additional","affiliation":[{"name":"Secretar\u00eda Regional Ministerial de Salud \u00d1uble,  Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3649-0676","authenticated-orcid":false,"given":"Pablo","family":"Oyarz\u00fan-Ruiz","sequence":"additional","affiliation":[{"name":"Universidad de Concepci\u00f3n,  Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0516-5667","authenticated-orcid":false,"given":"Mar\u00eda Carolina Silva-de la","family":"Fuente","sequence":"additional","affiliation":[{"name":"Universidad Austral de Chile,  Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7398-6099","authenticated-orcid":false,"given":"Carlos","family":"Landaeta-Aqueveque","sequence":"additional","affiliation":[{"name":"Universidad de Concepci\u00f3n,  Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2021]]},"reference":[{"key":"ref1","series-title":"Norma general t\u00e9cnica sobre inspeccion medico veterinaria de las reses de abasto y de sus carnes y criterios para la calificaci\u00f3n de aptitud para el consumo 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