{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T19:01:54Z","timestamp":1774378914008,"version":"3.50.1"},"reference-count":38,"publisher":"FapUNIFESP (SciELO)","issue":"2","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract In this study, whole blood samples and ticks were collected from 57 capybaras in recreational areas in the Federal District, Brazil, aiming to investigate the presence of Rickettsia spp. using polymerase chain reaction (PCR) and indirect immunofluorescence (IFAT) assays. None of the capybara blood samples yielded rickettsial DNA by PCR. Among 55 capybara serum samples tested by IFAT, 53 (96.3%) reacted to Rickettsia spp. Among these, 21 (39.6%) identified the R. bellii antigen as the probable antigen involved in a homologous reaction (PAIHR), whereas 2 (3.8%) identified the R. parkeri antigen. Ticks collected from capybaras were identified as 173 Amblyomma sculptum and 410 A. dubitatum, in addition to nine Amblyomma spp. larvae. A sample of 231 ticks was subjected to DNA extraction and PCR for Rickettsia species. None of 122 A. sculptum yielded rickettsial DNA. Molecular evidence of R. bellii was found in 25\/108 (23.1%) and of Rickettsia sp. strain Cooperi (R. parkeri-like agent) in 2\/108 (1.9%) of the A. dubitatum samples. These results suggest a greater exposure to R. bellii in these capybara populations, in addition to a more significant number of A. dubitatum, which might characterize the Federal District region as not endemic for Brazilian spotted fever.<\/jats:p>","DOI":"10.1590\/s1984-29612021035","type":"journal-article","created":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T16:00:21Z","timestamp":1622217621000},"source":"Crossref","is-referenced-by-count":6,"title":["Capybara (Hydrochoerus hydrochaeris) exposure to Rickettsia in the Federal District of Brazil, a non-endemic area for Brazilian spotted fever"],"prefix":"10.1590","volume":"30","author":[{"given":"Ana Paula Nunes de","family":"Quadros","sequence":"first","affiliation":[{"name":"Universidade de Bras\u00edlia,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George Magno Sousa do","family":"R\u00eago","sequence":"additional","affiliation":[{"name":"Universidade de Bras\u00edlia,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thamiris Figueiredo da","family":"Silva","sequence":"additional","affiliation":[{"name":"Universidade de Bras\u00edlia,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9a de Moraes","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Universidade de Bras\u00edlia,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thiago Fernandes","family":"Martins","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lina de Campos","family":"Binder","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rcio Botelho de","family":"Castro","sequence":"additional","affiliation":[{"name":"Universidade de Bras\u00edlia,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo S\u00e9rgio Ribeiro de","family":"Mattos","sequence":"additional","affiliation":[{"name":"Empresa Brasileira de Pesquisa Agropecu\u00e1ria,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo Bahia","family":"Labruna","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9112-1718","authenticated-orcid":false,"given":"Giane Regina","family":"Paludo","sequence":"additional","affiliation":[{"name":"Universidade de Bras\u00edlia,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2021]]},"reference":[{"issue":"5","key":"ref1","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1590\/S0103-84782000000500012","article-title":"Valores hematol\u00f3gicos de capivaras (Hydrochoerus hydrochaeris) criadas em cativeiro no munic\u00edpio de Botucatu, SP","volume":"30","author":"Arouca ME","year":"2000","journal-title":"Cienc Rural"},{"key":"ref2","series-title":"Carrapatos de import\u00e2ncia m\u00e9dico-veterin\u00e1ria da regi\u00e3o neotropical: um guia ilustrado para identifica\u00e7\u00e3o de esp\u00e9cies.","author":"Barros-Battesti DM","year":"2006"},{"issue":"9","key":"ref3","doi-asserted-by":"crossref","first-page":"4172","DOI":"10.1128\/JCM.41.9.4172-4177.2003","article-title":"Development and evaluation of a semi-nested PCR for detection and differentiation of Babesia gibsoni Asian Genotype) and B. canis DNA in Canine Blood Samples","volume":"41","author":"Birkenheuer AJ","year":"2017","journal-title":"J Clin Microbiol"},{"key":"ref4","series-title":"Casos confirmados de Febre Maculosa. Brasil, Grandes Regi\u00f5es e Unidades Federais. 2000 a 2019","year":"2019"},{"key":"ref5","series-title":"\u00d3bitos de Febre Maculosa. Brasil, Grandes Regi\u00f5es e Unidades Federais. 2000 a 2019","year":"2019"},{"issue":"5","key":"ref6","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1590\/1678-4162-9774","article-title":"Diferencia\u00e7\u00e3o morfom\u00e9trica entre larvas de Amblyomma sculptum Berlese, 1888 e Amblyomma dubitatum Neumann, 1899","volume":"70","author":"Brites-Neto J","year":"2018","journal-title":"Arq Bras Med Vet Zootec"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1093\/jme\/tjz141","article-title":"Rickettsia rickettsii (Rickettsiales: Rickettsiaceae) infecting Amblyomma sculptum (Acari: Ixodidae) ticks and capybaras in a Brazilian spotted fever-endemic area of Brazil","volume":"57","author":"Costa FB","year":"2019","journal-title":"J Med Entomol"},{"issue":"10","key":"ref8","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/j.tig.2007.08.002","article-title":"Intracellular pathogens go extreme: genome evolution in the Rickettsiales","volume":"23","author":"Darby AC","year":"2007","journal-title":"Trends Genet"},{"issue":"8","key":"ref9","doi-asserted-by":"crossref","first-page":"5569","DOI":"10.1128\/AEM.00122-06","article-title":"Isolation and identification of Rickettsia massiliae from Rhipicephalus sanguineus ticks collected in Arizona","volume":"72","author":"Eremeeva ME","year":"2006","journal-title":"Appl Environ Microbiol"},{"key":"ref10","series-title":"Secretaria de Sa\u00fade apura \u2018ataque\u2019 de carrapatos a visitantes do Zoo do DF","year":"2017"},{"issue":"6","key":"ref11","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1051\/vetres:2004038","article-title":"Determination of an efficient and reliable method for DNA extraction from ticks","volume":"35","author":"Halos L","year":"2004","journal-title":"Vet Res"},{"issue":"6","key":"ref12","doi-asserted-by":"crossref","first-page":"1492","DOI":"10.1093\/jme\/tjw111","article-title":"Development of a Rickettsia bellii-specific TaqMan assay targeting the citrate synthase gene","volume":"53","author":"Hecht JA","year":"2016","journal-title":"J Med Entomol"},{"issue":"7","key":"ref13","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1590\/S0074-02762007000700003","article-title":"Rickettsia infection in five areas of the state of S\u00e3o Paulo, Brazil","volume":"102","author":"Horta MC","year":"2007","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"93","DOI":"10.4269\/ajtmh.2004.71.93","article-title":"Prevalence of antibodies to spotted fever group rickettsiae in humans and domestic animals in a Brazilian spotted fever-endemic area in the state of S\u00e3o Paulo, Brazil: serologic evidence for infection by Rickettsia rickettsii and another spotted fever group Rickettsia.","volume":"71","author":"Horta MC","year":"2004","journal-title":"Am J Trop Med Hyg"},{"issue":"11","key":"ref15","doi-asserted-by":"crossref","first-page":"2715","DOI":"10.1128\/JCM.35.11.2715-2727.1997","article-title":"Laboratory diagnosis of Rickettsioses: current approaches todiagnosis of old and new Rickettsial diseases","volume":"35","author":"La Scola B","year":"1997","journal-title":"J Clin Microbiol"},{"issue":"1","key":"ref16","doi-asserted-by":"crossref","first-page":"77","DOI":"10.3157\/0013-872X(2007)118[77:HPBTCT]2.0.CO;2","article-title":"Human parasitism by the capybara tick, Amblyomma dubitatum (Acari: ixodidae)","volume":"118","author":"Labruna MB","year":"2007","journal-title":"Entomol News"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1128\/JCM.42.1.90-98.2004","article-title":"Rickettsia species infecting Amblyomma cooperi ticks from an area in the state of S\u00e3o Paulo, Brazil, where Brazilian spotted fever is endemic","volume":"42","author":"Labruna MB","year":"2004","journal-title":"J Clin Microbiol"},{"issue":"5","key":"ref18","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1016\/j.ttbdis.2016.05.011","article-title":"Comparative value of blood and skin samples for diagnosis of spotted fever group rickettsial infection in model animals","volume":"7","author":"Levin ML","year":"2016","journal-title":"Ticks Tick Borne Dis"},{"issue":"9","key":"ref19","article-title":"Epidemiology of capybara-associated Brazilian spotted fever","volume":"13","author":"Luz HR","year":"2019","journal-title":"PLoS Negl Trop Dis"},{"issue":"6","key":"ref20","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1603\/0022-2585-39.6.809","article-title":"Rickettsial infection in Dermacentor variabilis (Acari: Ixodidae) inhibits transovarial transmission of a second Rickettsia.","volume":"39","author":"Macaluso KR","year":"2002","journal-title":"J Med Entomol"},{"issue":"4","key":"ref21","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1590\/S0103-84782006000400046","article-title":"Valores hematol\u00f3gicos de capivaras (Hydrochoerus hydrochaeris) Rodentia:Hydrochoeridae) de vida livre na regi\u00e3o de Campinas-SP","volume":"36","author":"Madella DA","year":"2006","journal-title":"Cienc Rural"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1186\/s13071-016-1460-2","article-title":"Geographical distribution of Amblyomma cajennense (sensu lato) ticks (Parasitiformes: Ixodidae) in Brazil, with description of the nymph of A. cajennense (sensu stricto)","volume":"9","author":"Martins TF","year":"2016","journal-title":"Parasit Vectors"},{"issue":"2","key":"ref23","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.ttbdis.2010.03.002","article-title":"Nymphs of the genus Amblyomma (Acari: Ixodidae) of Brazil: descriptions, redescriptions, and identification key","volume":"1","author":"Martins TF","year":"2010","journal-title":"Ticks Tick Borne Dis"},{"key":"ref24","series-title":"Crian\u00e7as v\u00e3o ao Zoo do DF e voltam para casa infestadas de carrapatos","author":"Medeiros B","year":"2018"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1089\/vbz.2014.1741","article-title":"Molecular Detection of the Human Pathogenic Rickettsia sp. Strain Atlantic Rainforest in Amblyomma dubitatum Ticks from Argentina","volume":"15","author":"Monje LD","year":"2015","journal-title":"Vector Borne Zoonotic Dis"},{"key":"ref26","series-title":"Estudio Biologico del Chig\u00fcire o Capibara.","author":"Ojasti J","year":"1973"},{"issue":"3","key":"ref27","doi-asserted-by":"crossref","first-page":"364","DOI":"10.7705\/biomedica.v27i3.199","article-title":"Rickettsial infection in capybaras (Hydrochoerus hydrochaeris) from S\u00e3o Paulo, Brazil: serological evidence for infection by Rickettsia bellii and Rickettsia parkeri.","volume":"27","author":"Pacheco RC","year":"2007","journal-title":"Biomedica"},{"issue":"3","key":"ref28","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1590\/S0037-86822009000300023","article-title":"Survey of Rickettsia spp in the ticks Amblyomma cajennense and Amblyomma dubitatum in the State of S\u00e3o Paulo","volume":"42","author":"Pacheco RC","year":"2009","journal-title":"Rev Soc Bras Med Trop"},{"issue":"6","key":"ref29","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1086\/381894","article-title":"Rickettsia parkeri: a newly recognized cause of spotted fever rickettsiosis in the united states","volume":"38","author":"Paddock CD","year":"2004","journal-title":"Clin Infect Dis"},{"issue":"4","key":"ref30","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1128\/CMR.18.4.719-756.2005","article-title":"Tick-Borne Rickettsioses around the World: emerging diseases challenging old concepts","volume":"18","author":"Parola P","year":"2005","journal-title":"Clin Microbiol Rev"},{"issue":"6","key":"ref31","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1016\/j.ttbdis.2014.07.003","article-title":"Experimental infection with Rickettsia rickettsii in an Amblyomma dubitatum tick colony, naturally infected by Rickettsia bellii.","volume":"5","author":"Sakai RK","year":"2014","journal-title":"Ticks Tick Borne Dis"},{"issue":"2","key":"ref32","doi-asserted-by":"crossref","first-page":"265","DOI":"10.3201\/eid1102.040656","article-title":"Rickettsial infection in animals and Brazilian spotted fever endemicity","volume":"11","author":"Sangioni LA","year":"2005","journal-title":"Emerg Infect Dis"},{"issue":"7","key":"ref33","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.3201\/eid1307.061397","article-title":"Rickettsia parkeri in Brazil","volume":"13","author":"Silveira I","year":"2007","journal-title":"Emerg Infect Dis"},{"issue":"16","key":"ref34","doi-asserted-by":"crossref","first-page":"3424","DOI":"10.1017\/S095026881700245X","article-title":"Anaplasmataceae agents among wild mammals and ectoparasites in Brazil","volume":"145","author":"Sousa KCM","year":"2017","journal-title":"Epidemiol Infect"},{"issue":"1&#8211;2","key":"ref35","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.vetpar.2008.12.010","article-title":"Experimental infection of capybaras Hydrochoerus hydrochaeris by Rickettsia rickettsii and evaluation of the transmission of the infection to ticks Amblyomma cajennense.","volume":"161","author":"Souza CE","year":"2009","journal-title":"Vet Parasitol"},{"issue":"6","key":"ref36","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1017\/S0031182012002065","article-title":"In vitro isolation from Amblyomma ovale (Acari: Ixodidae) and ecological aspects of the Atlantic rainforest Rickettsia, the causative agent of a novel spotted fever rickettsiosis in Brazil","volume":"140","author":"Szab\u00f3 MP","year":"2013","journal-title":"Parasitology"},{"issue":"8","key":"ref37","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.3201\/eid1008.030999","article-title":"Rickettsia parkeri in Amblyomma triste from Uruguay","volume":"10","author":"Venzal JM","year":"2004","journal-title":"Emerg Infect Dis"},{"key":"ref38","series-title":"Veterinary hematology and clinical chemistry.","first-page":"3","volume-title":"Laboratory technology for veterinary medicine.","author":"Weiser G","year":"2012","edition":"2nd"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rbpv\/v30n2\/1984-2961-rbpv-30-2-e028720.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T16:00:50Z","timestamp":1622217650000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612021000200309&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"references-count":38,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2021]]}},"alternative-id":["S1984-29612021000200309"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612021035","relation":{},"ISSN":["1984-2961","0103-846X"],"issn-type":[{"value":"1984-2961","type":"electronic"},{"value":"0103-846X","type":"print"}],"subject":[],"published":{"date-parts":[[2021]]},"article-number":"e028720"}}